| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,507,263 | C→T | 32.2% | S109S (AGC→AGT) | yicI → | predicted alpha‑glucosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,507,263 | 0 | C | T | 32.2% | 89.5 / 51.2 | 102 | S109S (AGC→AGT) | yicI | predicted alpha‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base C (49/20); new base T (12/21); total (61/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.18e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
GACGTGAAGGTCACAATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGT > minE/2507201‑2507320 | gACGTGAAGGTCACAATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGTCAACTTAAGCGCGCgt > 1:3179520/1‑69 (MQ=255)gACGTGAAGGTCACAATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGcgcgcg > 1:1129084/1‑68 (MQ=255)gACGTGAAGGTCACAATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCgt > 1:489876/1‑69 (MQ=255)gACGTGAAGGTCACAATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCgt > 1:1897690/1‑69 (MQ=255)gACGTGAAGGTCACAATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCgt > 1:3284518/1‑69 (MQ=255)gACGTGAAGGTCACAATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCgt > 1:132678/1‑69 (MQ=255)gACGTGAAGGTCACAATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCgt > 1:2162254/1‑69 (MQ=255) cacaATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAg > 1:814985/1‑68 (MQ=255) cacaATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAg > 1:1770684/1‑68 (MQ=255) cacaATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAg > 1:1624888/1‑68 (MQ=255) cacaATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAg > 1:1914528/1‑68 (MQ=255) cacaATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAg > 1:1994488/1‑68 (MQ=255) cacaATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAg > 1:2024738/1‑68 (MQ=255) cacaATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAg > 1:263062/1‑68 (MQ=255) cacaATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAg > 1:2755350/1‑68 (MQ=255) cacaATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAg > 1:2944247/1‑68 (MQ=255) cacaATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAg > 1:1419595/1‑68 (MQ=255) cacaATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAg > 1:1304547/1‑68 (MQ=255) acaATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAg < 1:1452021/62‑1 (MQ=255) acaATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAg > 1:2947342/1‑62 (MQ=255) acaATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAg > 1:309652/1‑62 (MQ=255) acaATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAg > 1:1157769/1‑62 (MQ=255) acaATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAg > 1:432307/1‑62 (MQ=255) aTCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAg > 1:2994554/1‑69 (MQ=255) tCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGt > 1:1045988/1‑69 (MQ=255) cGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGtt < 1:2531429/69‑1 (MQ=255) aaaCACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTg > 1:1787335/1‑69 (MQ=255) aaaCACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTg > 1:91102/1‑69 (MQ=255) aaaCACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTg > 1:1648499/1‑69 (MQ=255) aaaCACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTg > 1:3100804/1‑69 (MQ=255) aaaCACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTg > 1:1566955/1‑69 (MQ=255) aaaCACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTg > 1:1384797/1‑69 (MQ=255) aaaCACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTg > 1:2880656/1‑69 (MQ=255) aaaCACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTg > 1:1509960/1‑69 (MQ=255) cacaGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTc > 1:937209/1‑69 (MQ=255) cacaGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTc > 1:821821/1‑69 (MQ=255) cacaGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTc > 1:1040025/1‑69 (MQ=255) cacaGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTc > 1:330390/1‑69 (MQ=255) cacaGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTc > 1:1593936/1‑69 (MQ=255) cacaGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTc > 1:2084951/1‑69 (MQ=255) tATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGAtttt > 1:1618986/1‑69 (MQ=255) gAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGa > 1:2125865/1‑59 (MQ=255) gAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGa > 1:3062337/1‑59 (MQ=255) gAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGa > 1:2595002/1‑59 (MQ=255) gAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTgcgc < 1:755344/69‑1 (MQ=255) gAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTgcgc < 1:813043/69‑1 (MQ=255) gAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTgcgc < 1:1155296/69‑1 (MQ=255) aGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCa > 1:2395023/1‑69 (MQ=255) aGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCa > 1:885636/1‑69 (MQ=255) ttAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACg < 1:583862/69‑1 (MQ=255) ttAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACg < 1:444888/69‑1 (MQ=255) ttAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACg < 1:1167097/69‑1 (MQ=255) tAAAAGTGGTAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACg > 1:1445236/1‑68 (MQ=255) tAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTc > 1:2063608/1‑43 (MQ=255) tAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACg > 1:840686/1‑68 (MQ=255) tAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACg > 1:39085/1‑68 (MQ=255) tAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACg > 1:1715983/1‑68 (MQ=255) aGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGaa < 1:2974045/69‑1 (MQ=255) aGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGaa < 1:2133229/69‑1 (MQ=255) aGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGaa < 1:1873056/69‑1 (MQ=255) aGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGaa < 1:718783/69‑1 (MQ=255) aGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGaa < 1:1093271/69‑1 (MQ=255) aGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGaa < 1:337659/69‑1 (MQ=255) aGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGaa < 1:3368444/69‑1 (MQ=255) aGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGaa < 1:1208023/69‑1 (MQ=255) aGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGaa < 1:2898133/69‑1 (MQ=255) aGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGaa < 1:3154166/69‑1 (MQ=255) aGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACCGCGaa < 1:766100/69‑1 (MQ=255) ggCAACTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGt < 1:2392055/69‑1 (MQ=255) ggCAACTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGt < 1:2955594/69‑1 (MQ=255) ggCAACTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGt < 1:2102190/69‑1 (MQ=255) ggCAACTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGt < 1:2317878/69‑1 (MQ=255) ggCAACTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGt < 1:834046/69‑1 (MQ=255) ggCAACTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGt < 1:1875381/69‑1 (MQ=255) ggCAACTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGt < 1:814865/69‑1 (MQ=255) ggCAACTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGt < 1:788581/69‑1 (MQ=255) ggCAACTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGt < 1:2345969/69‑1 (MQ=255) ggCAACTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGt < 1:3084847/69‑1 (MQ=255) ggCAACTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGt < 1:312703/69‑1 (MQ=255) ggCAACTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGt < 1:426623/69‑1 (MQ=255) ggCAACTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGt < 1:1455534/69‑1 (MQ=255) ggCAACTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGt < 1:3198452/69‑1 (MQ=255) ggCAACTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGt < 1:388579/69‑1 (MQ=255) ggCAACTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGt < 1:3383150/69‑1 (MQ=255) ggCAACTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGt < 1:1461732/69‑1 (MQ=255) ggCAACTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGt < 1:3341496/69‑1 (MQ=255) ggCAACTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGt < 1:192343/69‑1 (MQ=255) gCAACTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGt < 1:2854851/68‑1 (MQ=255) aaCTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTg > 1:2327484/1‑42 (MQ=255) aaCTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTg > 1:1326446/1‑42 (MQ=255) aaCTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTg > 1:873565/1‑42 (MQ=255) aaCTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTg > 1:3273358/1‑42 (MQ=255) aaCTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTg > 1:816881/1‑42 (MQ=255) aaCTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTg > 1:2323516/1‑42 (MQ=255) aaCTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTg > 1:810398/1‑42 (MQ=255) aaCTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTg > 1:1282742/1‑42 (MQ=255) aaCTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTg > 1:691744/1‑42 (MQ=255) aaCTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTg > 1:47921/1‑42 (MQ=255) aaCTTAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTg > 1:2618305/1‑42 (MQ=255) ttAAGTGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGt < 1:2076685/63‑1 (MQ=255) | GACGTGAAGGTCACAATCGAAAACACAGAACGTTATGCTGAGTTTAAAAGTGGCAACTTAAGCGCGCGTGTCAGCAAAGGTGAGTTCTGGTCACTGGATTTTCTGCGCAACGGCGAACGT > minE/2507201‑2507320 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |