| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 213,333 | C→T | 29.6% | G92S (GGC→AGC) | gloB ← | predicted hydroxyacylglutathione hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 213,333 | 0 | C | T | 29.6% | 83.9 / 36.8 | 84 | G92S (GGC→AGC) | gloB | predicted hydroxyacylglutathione hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (18/41); new base T (14/11); total (32/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.80e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
TCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGACCATACACCACAATTTG > minE/213268‑213396 | tCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCTCAAAACGAAGGCAGTTTCGCCATc > 1:1802469/1‑69 (MQ=255)tCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATc > 1:1660221/1‑69 (MQ=255)tCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATc > 1:791902/1‑69 (MQ=255)tCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATc > 1:705095/1‑69 (MQ=255)tCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATc > 1:701190/1‑69 (MQ=255)tCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATc > 1:2862810/1‑69 (MQ=255) aaaGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCttt < 1:937583/68‑1 (MQ=255) aaaGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCttt < 1:925011/68‑1 (MQ=255) aaaGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCttt < 1:1715914/68‑1 (MQ=255) gtgtGACCCGGCGTAGCAATTACACTAAATTTATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACt < 1:561658/69‑1 (MQ=255) gtgtGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACt < 1:3058530/69‑1 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCttt > 1:3120036/1‑58 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgtg > 1:2502182/1‑69 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgtg > 1:2778198/1‑69 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgtg > 1:202543/1‑69 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgtg > 1:3007148/1‑69 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgtg > 1:3318207/1‑69 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgtg > 1:404950/1‑69 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgtg > 1:633509/1‑69 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgtg > 1:131182/1‑69 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgtg > 1:694486/1‑69 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgtg > 1:1013461/1‑69 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgt > 1:2441252/1‑68 (MQ=255) ccGGTGTGGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgt > 1:2296028/1‑68 (MQ=255) ccGGTGTGGCAATTACACTAAATTAATGCCCCAAAACGAAGGCAGTTTCGCTATCTTTGACTACCtgtg > 1:3123746/1‑69 (MQ=255) aTTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTa < 1:163819/69‑1 (MQ=255) aTTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTa < 1:2624449/69‑1 (MQ=255) ttACTCTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTAt < 1:2307290/69‑1 (MQ=255) ttACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTa < 1:2686396/68‑1 (MQ=255) ttACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTa < 1:2852424/68‑1 (MQ=255) tACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATc < 1:2658454/69‑1 (MQ=255) tACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATc < 1:570206/69‑1 (MQ=255) acacTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCt > 1:2810361/1‑69 (MQ=255) acacTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCt > 1:2622411/1‑69 (MQ=255) acacTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCt > 1:981973/1‑69 (MQ=255) acacTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCt > 1:2417301/1‑69 (MQ=255) acacTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCt > 1:2129104/1‑69 (MQ=255) acacTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCt > 1:1445524/1‑69 (MQ=255) acacTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCt > 1:2093948/1‑69 (MQ=255) acacTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCt > 1:1847256/1‑69 (MQ=255) acTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCttt < 1:2744601/42‑1 (MQ=255) acTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCttt < 1:2863440/42‑1 (MQ=255) acTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCttt < 1:162023/42‑1 (MQ=255) acTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCttt < 1:1573079/42‑1 (MQ=255) acTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCttt < 1:1549029/42‑1 (MQ=255) acTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCttt < 1:2834152/42‑1 (MQ=255) acTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCttt < 1:2829174/42‑1 (MQ=255) acTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCttt < 1:380100/42‑1 (MQ=255) acTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCttt < 1:1958585/42‑1 (MQ=255) acTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCttt < 1:1363325/42‑1 (MQ=255) acTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCTATCttt < 1:215516/42‑1 (MQ=255) aaTTCATGCCCCAAAACGAAGGCAGTTTCTCCATCTTTGACTACCTGtgtt > 1:3207162/1‑51 (MQ=255) aaTTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGtc > 1:1121904/1‑69 (MQ=255) aaTTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCATATCTTGTGtc > 1:3243093/1‑69 (MQ=255) ttCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGtctc < 1:1657298/69‑1 (MQ=255) tCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTctct < 1:1279369/69‑1 (MQ=255) tCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTctct < 1:498211/69‑1 (MQ=255) tCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTctct < 1:337795/69‑1 (MQ=255) tCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTctct < 1:3309783/69‑1 (MQ=255) tCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTctct < 1:2845551/69‑1 (MQ=255) cATGCCCCATAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTctct < 1:1362724/68‑1 (MQ=255) cATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTctct < 1:1802339/68‑1 (MQ=255) cATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCtt < 1:3248519/69‑1 (MQ=255) cATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCtt < 1:1452715/69‑1 (MQ=255) cATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCtt < 1:3319180/69‑1 (MQ=255) cATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCtt < 1:1777227/69‑1 (MQ=255) cATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCtt < 1:1927640/69‑1 (MQ=255) cATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCtt < 1:116643/69‑1 (MQ=255) cATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCtt < 1:1207767/69‑1 (MQ=255) cATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCtt < 1:545269/69‑1 (MQ=255) cATGCCACAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCtt < 1:2441703/69‑1 (MQ=255) ccAAAACGAAGTCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGAc < 1:937701/69‑1 (MQ=255) ccAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGAc < 1:1147550/69‑1 (MQ=255) ccAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGAc < 1:1273822/69‑1 (MQ=255) ccAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGAc < 1:3054157/69‑1 (MQ=255) ccAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGAc < 1:1358231/69‑1 (MQ=255) ccAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGAc < 1:2110507/69‑1 (MQ=255) ccAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGAc < 1:37790/69‑1 (MQ=255) ccAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGAc < 1:3285527/69‑1 (MQ=255) ccAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGAc < 1:1769951/69‑1 (MQ=255) aaaaCGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGAc < 1:1189111/67‑1 (MQ=255) aCGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTctct < 1:388843/57‑1 (MQ=255) aGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCtt < 1:2603112/54‑1 (MQ=255) tCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGACCATACACCACAATTtg > 1:842517/1‑68 (MQ=255) | TCCTAAAGTGTGACCCGGCGTAGCAATTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGACCATACACCACAATTTG > minE/213268‑213396 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |