| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,507,377 | C→A | 16.3% | R147R (CGC→CGA) | yicI → | predicted alpha‑glucosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,507,377 | 0 | C | A | 16.3% | 238.9 / 26.6 | 110 | R147R (CGC→CGA) | yicI | predicted alpha‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base C (51/41); new base A (10/8); total (61/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
AACGGCGAACGTATTACCGGTAGTCAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTT > minE/2507309‑2507442 | aaCGGCGAACGTATTACCGGTAGTCAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGc < 1:635113/69‑1 (MQ=255)aaCGGCGAACGTATTACCGGTAGTCAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGc < 1:665042/69‑1 (MQ=255) cGGCGAACGTATTACCGGTAGTCAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGc < 1:485543/67‑1 (MQ=255) cGTATTACCGGTAGTCAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATg < 1:1794738/69‑1 (MQ=255) cGTATTACCGGTAGTCAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATg < 1:319349/69‑1 (MQ=255) cGTATTACCGGTAGTCAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATg < 1:2325331/69‑1 (MQ=255) cGTATTACCGGTAGTCAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATg < 1:1394322/69‑1 (MQ=255) cGTATTACCGGTAGTCAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATg < 1:726748/69‑1 (MQ=255) cGTATTACCGGTAGTCAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATg < 1:501138/69‑1 (MQ=255) cGTATTACCGGTAGTCAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATg < 1:2737493/69‑1 (MQ=255) cGTATTACCGGTAGTCAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATg < 1:1925722/69‑1 (MQ=255) ttACCGGTAGTCAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATGTTTg < 1:1961821/69‑1 (MQ=255) ttACCGGTAGTCAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATGTTTg < 1:1210441/69‑1 (MQ=255) ttACCGGTAGTCAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATGTTTg < 1:3345390/69‑1 (MQ=255) ttACCGGTAGTCAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATGTTTg < 1:2004933/69‑1 (MQ=255) ttACCGGTAGTCAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATGTTTg < 1:561539/69‑1 (MQ=255) gTCAGGTGAAAAATAACGGCTACGTGCAGGACACGAATAATCAACGAAATTATATGTTTGAGCGGCtgg < 1:70244/69‑3 (MQ=255) gTCAGGTGAAAAATAACGGCTACGTGCAGGACACGAATAATCAACGAAATTATATGTTTGAGCGGCtgg < 1:3148444/69‑3 (MQ=255) gTCAGGTGAAAAATAACGGCTACGTGCAGGACACGAATAATCAACGAAATTATATGTTTGAGCGGCtgg < 1:803832/69‑3 (MQ=255) gTCAGGTGAAAAATAACGGCTACGTGCAGGACACGAATAATCAACGAAATTATATGTTTGAGCGGCtgg < 1:3114046/69‑3 (MQ=255) gTCAGGTGAAAAATAACGGCTACGTGCAGGACACGAATAATCAACGAAATTATATGTTTGAGCGGCtgg < 1:1375056/69‑3 (MQ=255) gTCAGGTGAAAAATAACGGCTACGTGCAGGACACGAATAATCAACGAAATTATATGTTTGAGCGGCtgg < 1:2944904/69‑3 (MQ=255) tCAGGTGAAAAATAACGGCTACGTGCAGGACACGAATAATCAACGAAATTATATGTTTGAGCGGCtgg < 1:20567/68‑3 (MQ=255) cAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAATGCAATTATATGTTTGAGCGGCTTGAt < 1:2416938/69‑1 (MQ=255) cAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGa < 1:3414201/68‑1 (MQ=255) cAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGa < 1:47890/68‑1 (MQ=255) cAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGAt < 1:2956187/69‑1 (MQ=255) aaaaaTAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATg < 1:1569757/48‑1 (MQ=255) aaaaaTAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATGTTTg < 1:671370/52‑1 (MQ=255) gCTACGTGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGa > 1:1164714/1‑67 (MQ=255) gCTACGAGCAGGACACGAATAATCAACGCAATTATATg > 1:2566934/1‑38 (MQ=255) tGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCTAAACAGtt < 1:1655893/68‑1 (MQ=255) tGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGtt < 1:1971967/68‑1 (MQ=255) tGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGtt < 1:52038/68‑1 (MQ=255) tGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGtt < 1:3147649/68‑1 (MQ=255) tGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGtt < 1:3307989/68‑1 (MQ=255) cacGAATAATGAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTc > 1:3096888/1‑68 (MQ=255) cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTc > 1:1935345/1‑68 (MQ=255) cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:2218771/1‑69 (MQ=255) cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:29154/1‑69 (MQ=255) cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:1344042/1‑69 (MQ=255) cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:1698780/1‑69 (MQ=255) cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:2508926/1‑69 (MQ=255) cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:1730866/1‑69 (MQ=255) cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:873635/1‑69 (MQ=255) cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:3113676/1‑69 (MQ=255) cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:760685/1‑69 (MQ=255) cacGAATAATCAACGAAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:717590/1‑69 (MQ=255) cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:248460/1‑69 (MQ=255) cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:3022756/1‑69 (MQ=255) cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:740541/1‑69 (MQ=255) cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:2916489/1‑69 (MQ=255) cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:2256249/1‑69 (MQ=255) cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:1137578/1‑69 (MQ=255) cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:2851061/1‑69 (MQ=255) cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:701757/1‑69 (MQ=255) cGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTgg < 1:2459118/69‑1 (MQ=255) cGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTgg < 1:1689772/69‑1 (MQ=255) cGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTgg < 1:1024548/69‑1 (MQ=255) cGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTgg < 1:1457471/69‑1 (MQ=255) cGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTgg < 1:1497778/69‑1 (MQ=255) cGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTgg < 1:2287538/69‑1 (MQ=255) cGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTgg < 1:2405934/69‑1 (MQ=255) atCGACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:3206166/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:2314269/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:95409/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:1224251/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:1311844/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:919806/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:359295/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:2617891/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:1660893/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:3304985/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:3161220/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:2006309/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:2165112/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACTGTCTGGGAGAgc > 1:3347727/1‑69 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgc > 1:2502141/1‑69 (MQ=255) tCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcg > 1:683572/1‑69 (MQ=255) tCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcg > 1:2607325/1‑69 (MQ=255) tCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcg > 1:1699678/1‑69 (MQ=255) tCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcg > 1:1232426/1‑69 (MQ=255) tCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcg > 1:2637837/1‑69 (MQ=255) tCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTAGGCTAAACAGTTTa > 1:979788/1‑54 (MQ=255) cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:588346/1‑69 (MQ=255) cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:3089401/1‑69 (MQ=255) cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:2899593/1‑69 (MQ=255) cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:2792336/1‑69 (MQ=255) cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:721288/1‑69 (MQ=255) cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:2181073/1‑69 (MQ=255) cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:1906576/1‑69 (MQ=255) cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:1800433/1‑69 (MQ=255) cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:1316207/1‑69 (MQ=255) cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:111518/1‑69 (MQ=255) cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:1092243/1‑69 (MQ=255) cAACGCAATAATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:2498680/1‑69 (MQ=255) cGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCtt < 1:3117087/68‑1 (MQ=255) cGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCtt < 1:2901238/68‑1 (MQ=255) cGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCtt < 1:283386/68‑1 (MQ=255) gCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACATTTTACGGTCTGGGAGAGCTCtt < 1:2330424/67‑1 (MQ=255) | AACGGCGAACGTATTACCGGTAGTCAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTT > minE/2507309‑2507442 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |