| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,507,398 | T→G | 22.4% | L154L (CTT→CTG) | yicI → | predicted alpha‑glucosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,507,398 | 0 | T | G | 22.4% | 190.5 / 58.8 | 125 | L154L (CTT→CTG) | yicI | predicted alpha‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base T (69/28); new base G (9/19); total (78/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.03e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
Only 100 of 125 total aligned reads displayed.
CAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTACTGCCCTGGTGCGCAATG > minE/2507333‑2507462 | cAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAATGCAATTATATGTTTGAGCGGCTTGAt < 1:2416938/69‑1 (MQ=255)cAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGa < 1:47890/68‑1 (MQ=255)cAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGa < 1:3414201/68‑1 (MQ=255) tGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCTAAACAGtt < 1:1655893/68‑1 (MQ=255) tGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGtt < 1:3307989/68‑1 (MQ=255) tGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGtt < 1:1971967/68‑1 (MQ=255) tGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGtt < 1:3147649/68‑1 (MQ=255) cacGAATAATGAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTc > 1:3096888/1‑68 (MQ=255) cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:1344042/1‑69 (MQ=255) cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:1698780/1‑69 (MQ=255) cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:2218771/1‑69 (MQ=255) cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:3113676/1‑69 (MQ=255) cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:873635/1‑69 (MQ=255) cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:3068512/1‑69 (MQ=255) cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:2508926/1‑69 (MQ=255) cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:2523930/1‑69 (MQ=255) cacGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:29154/1‑69 (MQ=255) cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGTCGTTGGCGAAACAGTTTACGGTCt > 1:2022340/1‑69 (MQ=255) cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:2851061/1‑69 (MQ=255) cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:2256249/1‑69 (MQ=255) cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:248460/1‑69 (MQ=255) cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:3022756/1‑69 (MQ=255) cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:2916489/1‑69 (MQ=255) cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:1137578/1‑69 (MQ=255) cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:701757/1‑69 (MQ=255) cacGAATAATCAACGAAATTATATGTTTGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCt > 1:740541/1‑69 (MQ=255) cGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTgg < 1:1024548/69‑1 (MQ=255) cGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTgg < 1:1689772/69‑1 (MQ=255) cGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTgg < 1:2459118/69‑1 (MQ=255) cGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTgg < 1:2405934/69‑1 (MQ=255) cGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTgg < 1:1497778/69‑1 (MQ=255) cGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTgg < 1:2287538/69‑1 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:359295/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:2617891/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:1224251/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:95409/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:919806/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:1305435/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:1311844/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:2314269/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:3161220/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:3191142/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:3304985/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:2165112/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCtt > 1:2006309/1‑35 (MQ=255) atCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACTGTCTGGGAGAgc > 1:3347727/1‑69 (MQ=255) tCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcg > 1:1232426/1‑69 (MQ=255) tCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcg > 1:1699678/1‑69 (MQ=255) tCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcg > 1:2607325/1‑69 (MQ=255) tCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcg > 1:683572/1‑69 (MQ=255) tCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTAGGCTAAACAGTTTa > 1:979788/1‑54 (MQ=255) cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:726950/1‑69 (MQ=255) cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:721288/1‑69 (MQ=255) cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:588346/1‑69 (MQ=255) cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:1092243/1‑69 (MQ=255) cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:2181073/1‑69 (MQ=255) cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:1316207/1‑69 (MQ=255) cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:2792336/1‑69 (MQ=255) cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:111518/1‑69 (MQ=255) cAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:1906576/1‑69 (MQ=255) cAACGCAATAATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAgcgc > 1:2498680/1‑69 (MQ=255) cGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCtt < 1:2901238/68‑1 (MQ=255) cGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCtt < 1:1887439/68‑1 (MQ=255) cGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCtt < 1:3117087/68‑1 (MQ=255) cGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCtt < 1:283386/68‑1 (MQ=255) gCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACATTTTACGGTCTGGGAGAGCTCtt < 1:2330424/67‑1 (MQ=255) aTTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAAGAGtt < 1:1751263/44‑1 (MQ=255) tataTGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACgg > 1:724179/1‑47 (MQ=255) tataTGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACgg > 1:178607/1‑47 (MQ=255) tataTGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTACTGcc > 1:2467645/1‑69 (MQ=255) tataTGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTACTGcc > 1:2239818/1‑69 (MQ=255) tataTGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTACTGcc > 1:812027/1‑69 (MQ=255) atGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTACTGCCCt < 1:524698/68‑1 (MQ=255) atGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTACTGCCCt < 1:875054/68‑1 (MQ=255) atGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTACTGCCCt < 1:96648/68‑1 (MQ=255) gTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCtt > 1:1492891/1‑57 (MQ=255) gTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCtt > 1:3161485/1‑57 (MQ=255) gTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCtt > 1:2220276/1‑57 (MQ=255) gTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCtt > 1:3010550/1‑57 (MQ=255) gTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCt > 1:1745728/1‑56 (MQ=255) tttGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTACTGCCCTGGTg > 1:1855232/1‑69 (MQ=255) tttGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTACTGCCCTGGTg > 1:293135/1‑69 (MQ=255) tttGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTAATGCCCTGGTg > 1:521300/1‑69 (MQ=255) ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:528572/56‑1 (MQ=255) ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:3056620/56‑1 (MQ=255) ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:1250929/56‑1 (MQ=255) ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:1299815/56‑1 (MQ=255) ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:2344030/56‑1 (MQ=255) ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:3178921/56‑1 (MQ=255) ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:3245127/56‑1 (MQ=255) ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:1496924/56‑1 (MQ=255) ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:1866407/56‑1 (MQ=255) ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:1674558/56‑1 (MQ=255) ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:710816/56‑1 (MQ=255) ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:2840034/56‑1 (MQ=255) ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:1787777/56‑1 (MQ=255) ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:623116/56‑1 (MQ=255) ttGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCttt < 1:490734/56‑1 (MQ=255) tGAGCGGCTGGATCTTGGCGTTGGCGAAACAGTATACGGTCTGGGAGAGCGCttt < 1:989268/55‑1 (MQ=39) aGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCtt < 1:2207454/52‑1 (MQ=255) | CAGGTGAAAAATAATGGCTACGTGCAGGACACGAATAATCAACGCAATTATATGTTTGAGCGGCTTGATCTTGGCGTTGGCGAAACAGTTTACGGTCTGGGAGAGCGCTTTACTGCCCTGGTGCGCAATG > minE/2507333‑2507462 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |