| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,508,933 | A→G | 55.1% | N666S (AAC→AGC) | yicI → | predicted alpha‑glucosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,508,933 | 0 | A | G | 55.1% | 7.9 / 36.4 | 49 | N666S (AAC→AGC) | yicI | predicted alpha‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base A (3/19); new base G (16/11); total (19/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.34e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
GACGGTAGTCGCTGGCATAAACAGCAGCACGGCTTCCTGAGTCTGCCCGTTTATGTGCGTGATAACACTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCC > minE/2508869‑2509001 | gACGGTAGTCGCTGGCATAAACAGCAGCACGGCTTCCTGAGTCTGCCCGTTTATGTGCGTGATAacac > 1:2634940/1‑68 (MQ=255) aGTCGCTGGCATAAACAGCAGCACGGCTTCCTGAGTCTGCCCGTTTATGTGCGTGATAACActc < 1:1443447/64‑1 (MQ=255) aGTCGCTGGCATAAACAGCAGCACGGCTTCCTGAGTCTGCCCGTTTATGTGCGTGATAACActc < 1:2385026/64‑1 (MQ=255) aGTCGCTGGCATAAACAGCAGCACGGCTTCCTGAGTCTGCCCGTTTATGTGCGTGATAACActc < 1:2326014/64‑1 (MQ=255) aGTCGCTGGCATAAACAGCAGCACGGCTTCCTGAGTCTGCCCGTTTATGTGCGTGATAACActc < 1:320757/64‑1 (MQ=255) aGTCGCTGGCATAAACAGCAGCACGGCTTCCTGAGTCTGCCCGTTTATGTGCGTGATAACActc < 1:785311/64‑1 (MQ=255) aGTCGCTGGCATAAACAGCAGCACGGCTTCCTGAGTCTGCCCGTTTATGTGCGTGATAACActc < 1:712987/64‑1 (MQ=255) aGTCGCTGGCATAAACAGCAGCACGGCTTCCTGAGTCTGCCCGTTTATGTGCGTGATAACActc < 1:1201576/64‑1 (MQ=255) aGTCGCTGGCATAAACAGCAGCACGGCTTCCTGAGTCTGCCCGTTTATGTGCGTGATAACACTCTACTg > 1:3104251/1‑69 (MQ=255) cGCTGGCATAAACAGCAGCACGGCTTCCTGAGTCTGCCCGTTTATGTGCGTGATAACACTCTACTGgcg < 1:1433163/69‑1 (MQ=255) gCTGGCATAAACAGCAGCACGGCTTCCTGAGTCTGCCCGTTTATGTGCGTGATAACACTCTACTGgcg < 1:3197401/68‑1 (MQ=255) aaaCAGCAGCACGGCTTCCTGAGTCTGCCCGTTTATGTGCGTGATAACACTCTACTGGCGCTGGGcaac < 1:2038430/69‑1 (MQ=255) aaCAGCAGCACGGCTTCCTGAGTCTGCCCGTTTATGTGCGTGATAACACTCTACTGGCGCTGGGcatca > 1:1377159/1‑69 (MQ=255) caCGGCTTCCTGAGTCTGCCCGTTTATGTGCGTGATAACACTCTACTGGCGCTGGGCAACAACGATCaa < 1:1307918/69‑1 (MQ=255) caCGGCTTCCTGAGTCTGCCCGTTTATGTGCGTGATAACACTCTACTGGCGCTGGGCAACAACGATCaa < 1:2614564/69‑1 (MQ=255) caCGGCTTCCTGAGTCTGCCCGTTTATGTGCGTGATAACACTCTACTGGCGCTGGGCAACAACGATCaa < 1:197266/69‑1 (MQ=255) caCGGCTTCCTGAGTCTGCCCGTTTATGTGCGTGATAACACTCTACTGGCGCTGGGCAACAACGATCaa < 1:1988900/69‑1 (MQ=255) caCGGCTTCCTGAGTCTGCCCGTTTATGTGCGTGATAACACTCTACTGGCGCTGGGCAACAACGATCaa < 1:2799362/69‑1 (MQ=255) ttCCTGAGTCTGCCCGTTTATGTGCGTGATAACACTCTACTGGCGCTGGGCAACAACGATCAACGTccc < 1:3216161/69‑1 (MQ=255) tttATGTGCGTGATAACACTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACg < 1:3223602/69‑1 (MQ=255) tATGTGCGTGATAACACTCTACTGGCGCTGGGcaac < 1:131984/36‑1 (MQ=255) cGTGATAACACTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCAc < 1:1483466/68‑1 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:484044/69‑1 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:732050/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:2566665/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:2614555/69‑1 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:481158/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:2821630/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:3069357/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:418241/69‑1 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:3198155/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:107203/69‑1 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:615236/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:1822817/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:1245171/69‑1 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:1313341/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:1352632/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:1442416/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:1235955/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:1205169/69‑1 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:186877/69‑1 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:1072849/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:2173725/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:2204001/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:2220210/69‑1 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:1055499/69‑1 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATAACGCGTGGCACGAAGGTACGGCa > 1:640421/1‑69 (MQ=255) aTAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:1182204/68‑1 (MQ=255) aTAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:2639065/68‑1 (MQ=255) aTAACACTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGc > 1:1623720/1‑67 (MQ=255) acacTCTAGTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:2318597/1‑69 (MQ=255) acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCa > 1:3137831/1‑60 (MQ=255) acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:3259043/1‑69 (MQ=255) acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:1571830/1‑69 (MQ=255) acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:3072350/1‑69 (MQ=255) acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:1101852/1‑69 (MQ=255) acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:1944094/1‑69 (MQ=255) acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:740447/1‑69 (MQ=255) acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:2325762/1‑69 (MQ=255) acacTCTACTGGCGCTGGGAAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:3148172/1‑69 (MQ=255) | GACGGTAGTCGCTGGCATAAACAGCAGCACGGCTTCCTGAGTCTGCCCGTTTATGTGCGTGATAACACTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCC > minE/2508869‑2509001 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |