| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,508,978 | T→C | 24.7% | V681A (GTG→GCG) | yicI → | predicted alpha‑glucosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,508,978 | 0 | T | C | 24.7% | 131.2 / 39.6 | 109 | V681A (GTG→GCG) | yicI | predicted alpha‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base T (66/16); new base C (16/11); total (82/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.89e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01 | |||||||||||
Only 100 of 109 total aligned reads displayed.
TTTATGTGCGTGATAACACTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCTGTGAAG > minE/2508918‑2509043 | tttATGTGCGTGATAACACTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACg < 1:3223602/69‑1 (MQ=255) cGTGATAACACTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCAc < 1:1483466/68‑1 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:615236/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:2173725/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:2204001/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:2220210/69‑1 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:186877/69‑1 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:1822817/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:484044/69‑1 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:481158/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:2566665/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:2614555/69‑1 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:1442416/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:1352632/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:1313341/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:2821630/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:1055499/69‑1 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:1235955/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:418241/69‑1 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:1205169/69‑1 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:107203/69‑1 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:732050/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:3069357/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:1072849/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa > 1:3198155/1‑69 (MQ=255) gATAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATAACGCGTGGCACGAAGGTACGGCa > 1:640421/1‑69 (MQ=255) aTAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:1182204/68‑1 (MQ=255) aTAGCACCCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGCGTGGCACGAAGGTACGGCa < 1:2639065/68‑1 (MQ=255) aTAACACTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGc > 1:1623720/1‑67 (MQ=255) acacTCTAGTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:2318597/1‑69 (MQ=255) acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCa > 1:3137831/1‑60 (MQ=255) acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:2325762/1‑69 (MQ=255) acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:3259043/1‑69 (MQ=255) acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:1944094/1‑69 (MQ=255) acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:740447/1‑69 (MQ=255) acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:1101852/1‑69 (MQ=255) acacTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:1571830/1‑69 (MQ=255) acacTCTACTGGCGCTGGGAAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTcc > 1:3148172/1‑69 (MQ=255) cTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTc > 1:2861873/1‑69 (MQ=255) cTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTc > 1:729121/1‑69 (MQ=255) ggCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCaa < 1:3253813/69‑1 (MQ=255) ggCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCaa < 1:2906974/69‑1 (MQ=255) ggCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCaa < 1:1541148/69‑1 (MQ=255) ggCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCaa < 1:2608217/69‑1 (MQ=255) ggCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCaa < 1:3197011/69‑1 (MQ=255) ggCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCaa > 1:910802/1‑69 (MQ=255) aacGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:1683683/1‑69 (MQ=255) aacGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:2317808/1‑69 (MQ=255) aacGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:416135/1‑69 (MQ=255) aacGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:2576321/1‑69 (MQ=255) aacGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:3244043/1‑69 (MQ=255) aacGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:537769/1‑69 (MQ=255) aacGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:1890866/1‑69 (MQ=255) aacGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:2771416/1‑69 (MQ=255) aacGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:2026996/1‑69 (MQ=255) cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:1110720/1‑69 (MQ=255) cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:3063920/1‑69 (MQ=255) cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:387121/1‑69 (MQ=255) cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:2107909/1‑69 (MQ=255) cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:421870/1‑69 (MQ=255) cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:129250/1‑69 (MQ=255) cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:2686236/1‑69 (MQ=255) cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:2574630/1‑69 (MQ=255) cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:2504865/1‑69 (MQ=255) cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:1673159/1‑69 (MQ=255) cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:2455679/1‑69 (MQ=255) cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:1753485/1‑69 (MQ=255) cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:1862198/1‑69 (MQ=255) cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:2306633/1‑69 (MQ=255) cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:515431/1‑69 (MQ=255) cAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGaa > 1:2131566/1‑69 (MQ=255) cAACGTACCGATTACGTGTGGCACGAAGGCACGGCATTCGACCTCTTCAATCTGCAAGACGGGCATGaa > 1:3264324/1‑69 (MQ=255) ccGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGc > 1:2280783/1‑64 (MQ=255) ccGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCt > 1:927763/1‑69 (MQ=255) ccGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCt > 1:1022797/1‑69 (MQ=255) ccGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCt > 1:1474011/1‑69 (MQ=255) ccGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCt > 1:216571/1‑69 (MQ=255) aTTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCaa < 1:936500/40‑1 (MQ=255) ttACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCTGTGa < 1:353464/69‑1 (MQ=255) ttACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCTGTGa < 1:2470848/69‑1 (MQ=255) tACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATg < 1:101775/55‑1 (MQ=255) tACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATg < 1:3028824/55‑1 (MQ=255) tACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATg < 1:887360/55‑1 (MQ=255) tACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATg < 1:729830/55‑1 (MQ=255) tACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATg < 1:2431797/55‑1 (MQ=255) tACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCTGTGaa > 1:1111395/1‑69 (MQ=255) aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCa > 1:718543/1‑36 (MQ=255) aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:2284997/1‑50 (MQ=255) aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:1214567/1‑50 (MQ=255) aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:132044/1‑50 (MQ=255) aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:1502416/1‑50 (MQ=255) aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:2275317/1‑50 (MQ=255) aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:3064284/1‑50 (MQ=255) aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:2952370/1‑50 (MQ=255) aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:2987437/1‑50 (MQ=255) aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:3421171/1‑50 (MQ=255) aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACggg > 1:3317357/1‑50 (MQ=255) aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCTGTGAAg > 1:2891393/1‑69 (MQ=255) aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCTGTGAAg > 1:397247/1‑69 (MQ=255) aCGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCTGTGAAg > 1:359224/1‑69 (MQ=255) | TTTATGTGCGTGATAACACTCTACTGGCGCTGGGCAACAACGATCAACGTCCCGATTACGTGTGGCACGAAGGCACGGCATTCCACCTCTTCAATCTGCAAGACGGGCATGAAGCCGTCTGTGAAG > minE/2508918‑2509043 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |