| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,509,148 | C→T | 66.3% | L738L (CTG→TTG) | yicI → | predicted alpha‑glucosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,509,148 | 0 | C | T | 66.3% | 60.8 / 44.9 | 83 | L738L (CTG→TTG) | yicI | predicted alpha‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base C (13/15); new base T (29/26); total (42/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCACGTACTGGCAACACGATTACTGTGACTGGTGCGGGCGAGGCGAAGAACTGGACACTGTGCCTGCGCAATGTTGTGAAAGTAAATGGTCTGCAAGACGGTTCGCAGGCTGAAAGTGAGCAGGGGCT > minE/2509085‑2509212 | gcaCGTACTGGCAACACGATTACTGTGACTGGTGCGGGCGAGGCGAAGAACTGGACACTGTGCCTgcgc < 1:1504965/69‑1 (MQ=255) caCGTACTGGCAACACGATTACTGTGACTGGTGCGGGCGAGGCGAAGAACTGGACACTGTGCCTGCGCa < 1:1849430/69‑1 (MQ=255) aCGTACTGGCAACACGATTACTGTGACTGGTGCGGGCGAGGCGAAGAACTGGACACTGTGCCTGCGCaa < 1:2505033/69‑1 (MQ=255) aCGTACTGGCAACACGATTACTGTGACTGGTGCGGGCGAGGCGAAGAACTGGACACTGTGCCTGCGCaa < 1:3097848/69‑1 (MQ=255) aCGTACTGGCAACACGATTACTGTGACTGGTGCGGGCGAGGCGAAGAACTGGACACTGTGCCTGCGCaa < 1:3053889/69‑1 (MQ=255) cGTACTGGCAACACGATTACTGTGACTGGTGCGGGCGAGGCGAAGAACTGGACACTGTGCCTGCGCaa < 1:1481837/68‑1 (MQ=255) cGTACTGGCAACACGATTACTGTGACTGGTGCGGGCGAGGCGAAGAACTGGACACTGTGCCTGCGCAAt > 1:2037247/1‑69 (MQ=255) aaCACGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAATATTGTGa < 1:2734777/67‑1 (MQ=255) aaCACGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAATATTGTGa < 1:1080776/67‑1 (MQ=255) aaCACGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAATATTGTGa < 1:2035368/67‑1 (MQ=255) aaCACGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAATATTGTGa < 1:2738803/67‑1 (MQ=255) aaCACGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAATATTGTGa < 1:2070728/67‑1 (MQ=255) aaCACGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAATATTGTGa < 1:289242/67‑1 (MQ=255) aaCACGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAATATTGTGa < 1:2197650/67‑1 (MQ=255) aaCACGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAATATTGTGa < 1:1044409/67‑1 (MQ=255) aaCACGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAATATTGTGa < 1:2278484/67‑1 (MQ=255) aaCACGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAATATTGTGa < 1:2474897/67‑1 (MQ=255) aaCACGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAATATTGTGa < 1:874757/67‑1 (MQ=255) aaCACGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAATATTGTGa < 1:1092534/67‑1 (MQ=255) aaCACGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAATATTGTGa < 1:3326869/67‑1 (MQ=255) aaCACGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAATATTGTGa < 1:853037/67‑1 (MQ=255) aaCACGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAATATTGTGa < 1:3422663/67‑1 (MQ=255) aaCACGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAATATTGTGa < 1:316369/67‑1 (MQ=255) aaCACGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAATATTGTGa < 1:600391/67‑1 (MQ=255) aaCACGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAATATTGTGa < 1:595979/67‑1 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:398036/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:2674164/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:72634/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:702073/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:620845/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:409378/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:2846957/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:2995227/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:2998929/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:3049378/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:3253052/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:3101167/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:3156095/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:2303844/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:1266846/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:1265991/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:1264489/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:1399222/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:1521006/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:157086/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:1579935/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:1612773/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:1705050/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:1765288/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:120970/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:1161699/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:183170/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:2267496/1‑62 (MQ=255) cacGATTACTGTGACTGGTACGGGCGAGGCGAAGAACTGGACACTGTGCTTGCGCAAtattg > 1:1854833/1‑62 (MQ=255) acGATTACTGTGACTGGTGCGGGCGAGGCGAAGAACTGGACACTGTGCCTGCGCAATGTTGTGAAAGTa < 1:3012197/69‑1 (MQ=255) acGATTACTGTGACTGGTGCGGGCGAGGCGAAGAACTGGACACTGTGCCTGCGCAATGTTGTGAAAGTa < 1:1735739/69‑1 (MQ=255) aTTACTGTGACTGGTGCGGGCGAGGCGAAGAACTGGACACTGTGCCTGCGCaa < 1:3133712/53‑1 (MQ=255) tgtgACTGGTGCGGGCGAGGCGAAGAACTGGACACTGTGCCTGCGCAATGTTGTGAAAGTAAATGGTc < 1:3201124/68‑1 (MQ=255) aggcgaAGAACTGGACACTGTGCCTGCGCAATGTTGTGAAAGTAAATgg < 1:2756211/49‑1 (MQ=255) cgaAGAACTGGACACTGTGCCTGCGCAATGTTGTGAAAGTAAATGGTc < 1:866811/48‑1 (MQ=255) ggACACTGTGCTTGCGCAATATTGTGAAAGTAAATGGTCTGCAAGGCGGTTCGCAGGCTGAAAGTGAGc < 1:689126/69‑1 (MQ=255) ggACACTGTGCTTGCGCAATATTGTGAAAGTAAATGGTCTGCAAGGCGGTTCGCAGGCTGAAAGTGAGc < 1:644015/69‑1 (MQ=255) ggACACTGTGCTTGCGCAATATTGTGAAAGTAAATGGTCTGCAAGGCGGTTCGCAGGCTGAAAGTGAGc < 1:64027/69‑1 (MQ=255) ggACACTGTGCTTGCGCAATATTGTGAAAGTAAATGGTCTGCAAGGCGGTTCGCAGGCTGAAAGTGAGc < 1:1138494/69‑1 (MQ=255) ggACACTGTGCTTGCGCAATATTGTGAAAGTAAATGGTCTGCAAGGCGGTTCGCAGGCTGAAAGTGAGc < 1:769266/69‑1 (MQ=255) ggACACTGTGCTTGCGCAATATTGTGAAAGTAAATGGTCTGCAAGGCGGTTCGCAGGCTGAAAGTGAGc < 1:3206983/69‑1 (MQ=255) ggACACTGTGCTTGCGCAATATTGTGAAAGTAAATGGTCTGCAAGGCGGTTCGCAGGCTGAAAGTGAGc < 1:3226738/69‑1 (MQ=255) ggACACTGTGCTTGCGCAATATTGTGAAAGTAAATGGTCTGCAAGGCGGTTCGCAGGCTGAAAGTGAGc < 1:2852436/69‑1 (MQ=255) acTGTGCCTGCGCAATGTTGTGAAAGTAAATGGTCTGCAAGACGGTTCGCAGGCTGAAAGTGAGCAggg < 1:1340436/69‑1 (MQ=255) acTGTGCCTGCGCAATGTTGTGAAAGTAAATGGTCTGCAAGACGGTTCGCAGGCTGAAAGTGAGCAggg < 1:1387206/69‑1 (MQ=255) acTGTGCCTGCGCAATGTTGTGAAAGTAAATGGTCTGCAAGACGGTTCGCAGGCTGAAAGTGAGCAggg < 1:65572/69‑1 (MQ=255) tgtgCCTGCGCAATGTTGTGAAAGTAAATGGTCTGCAAGACGGt > 1:2436586/1‑44 (MQ=255) tgtgCCTGCGCAATGTTGTGAAAGTAAATGGTCTGCAAGACGGTTCGCAGGCTGAAAGTGAGCAGGGGc > 1:1353358/1‑69 (MQ=255) tgtgCCTGCGCAATGTTGTGAAAGTAAATGGTCTGCAAGACGGTTCGCAGGCTGAAAGTGAGCAGGGGc > 1:980953/1‑69 (MQ=255) tgtgCCTGCGCAATGTTGTGAAAGTAAATGGTCTGCAAGACGGTTCGCAGGCTGAAAGTGAGCAGGGGc > 1:1083494/1‑69 (MQ=255) tgtgCCTGCGCAATGTTGTGAAAGTAAATGGTCTGCAAGACGGTTCGCAGGCTGAAAGTGAGCAGGGGc > 1:2619424/1‑69 (MQ=255) tgtgCCTGCGCAATGTTGTGAAAGTAAATGGTCTGCAAGACGGTTCGCAGGCTGAAAGTGAGCAGGGGc > 1:2793972/1‑69 (MQ=255) tgtgCCTGCGCAATGTTGTGAAAGTAAATGGTCTGCAAGACGGTTCGCAGGCTGAAAGTGAGCAGGGGc > 1:756589/1‑69 (MQ=255) tgtgCCTGCGCAATGTTGTGAAAGTAAATGGTCTGCAAGACGGTTCGCAGGCTGAAAGTGAGCAGGGGc > 1:729533/1‑69 (MQ=255) tgtgCCTGCGCAATGTTGTGAAAGTAAATGGTCTGCAAGACGGTTCGCAGGCTGAAAGTGAGCAGGGGc > 1:1796415/1‑69 (MQ=255) tgtgCCTGCGCAATGTTGTGAAAGTAAATGGTCTGCAAGACGGTTCGCAGGCTGAAAGAGAGCAgggg > 1:181539/1‑68 (MQ=255) gtgCCTGCGCAATGTTGTGAAAGTAAATGGTCTGCAAGACGGTTCGCAGGCTGAAAGTGAGCAGGGGCt > 1:2068110/1‑69 (MQ=255) gtgCCTGCGCAATGTTGTGAAAGTAAATGGTCTGCAAGACGGTTCGCAGGCTGAAAGTGAGCAGGGGCt > 1:1212580/1‑69 (MQ=255) | GCACGTACTGGCAACACGATTACTGTGACTGGTGCGGGCGAGGCGAAGAACTGGACACTGTGCCTGCGCAATGTTGTGAAAGTAAATGGTCTGCAAGACGGTTCGCAGGCTGAAAGTGAGCAGGGGCT > minE/2509085‑2509212 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |