| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,509,560 | T→G | 18.8% | T486T (ACA→ACC) | yicH ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,509,560 | 0 | T | G | 18.8% | 230.0 / 49.6 | 133 | T486T (ACA→ACC) | yicH | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (42/66); new base G (11/14); total (53/80) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 151 total aligned reads displayed.
AATTCAGGCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAGAATGCCTTTTTCAGTAAATGCA > minE/2509495‑2509626 | aaTTCAGGCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATgtgtgt > 1:1263453/1‑68 (MQ=255)aaTTCAGGCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATgtgt > 1:2244295/1‑68 (MQ=255)aaTTCAGGCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATgtgt > 1:1267081/1‑68 (MQ=255)aaTTCAGGCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATgtgt > 1:605041/1‑68 (MQ=255)aaTTCAGGCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATgtgt > 1:1886344/1‑68 (MQ=255)aaTTCAGGCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATgtgt > 1:917017/1‑68 (MQ=255)aaTTCAGGCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATgtgt > 1:2222269/1‑68 (MQ=255)aaTTCAGGCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATgtgt > 1:1881944/1‑68 (MQ=255)aaTTCAGGCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATgtgt > 1:721272/1‑68 (MQ=255)aaTTCAGGCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATgtgt > 1:2908459/1‑68 (MQ=255)aaTTCAGGCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATgtgt > 1:1704060/1‑68 (MQ=255)aaTTCAGGCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATgtgt > 1:1514713/1‑68 (MQ=255)aaTTAAGGCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATgtgt > 1:1003839/1‑68 (MQ=255) cAGGCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGAc > 1:2924292/1‑69 (MQ=255) cAGGCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGAc > 1:1935922/1‑69 (MQ=255) cAGGCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGAc > 1:51919/1‑69 (MQ=255) cAGGCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGAc > 1:1453734/1‑69 (MQ=255) cAGGCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGAc > 1:84779/1‑69 (MQ=255) cAGGCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGAc > 1:513542/1‑69 (MQ=255) cAGGCCATCCCCACTGTTGCAAAATATTCAACGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGAc > 1:3342012/1‑69 (MQ=255) gCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGt < 1:1560419/68‑1 (MQ=255) caaTCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGt < 1:3081879/65‑1 (MQ=255) cccACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCg > 1:2369138/1‑69 (MQ=255) cccACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCg > 1:2022314/1‑69 (MQ=255) cccACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCg > 1:492756/1‑69 (MQ=255) cccACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCg > 1:228959/1‑69 (MQ=255) cccACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCg > 1:2493625/1‑69 (MQ=255) cccACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCg > 1:787794/1‑69 (MQ=255) cccACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCg > 1:3184010/1‑69 (MQ=255) cccACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCg > 1:1495602/1‑69 (MQ=255) cccACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCg > 1:2732277/1‑69 (MQ=255) atGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGGGTCTGATGTTGTGGCGttt > 1:3255172/2‑68 (MQ=255) atGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGGGTCTGACGTTGTGGCGttt > 1:864724/2‑68 (MQ=255) atGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGGGTCTGACGTTGTGGCGttt > 1:1199575/2‑68 (MQ=255) atGTTGAAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGGGTCTGACGTTGTGGCGttt > 1:4138/2‑68 (MQ=255) gTTGCAAAATATTCACCGGCACACCGCTTCCATTCAGGCTGATATGGGTCTGACGTTGTGGCGTTtgtg < 1:2630070/69‑1 (MQ=255) gTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTtgt > 1:2667773/1‑68 (MQ=255) gTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGGGTCTGACGTTGTGGCGTTtgtg < 1:2949486/69‑1 (MQ=255) gTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGGGTCTGACGTTGTGGCGTTtgtg < 1:2962923/69‑1 (MQ=255) gTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGGGTCTGACGTTGTGGCGTTtgtg < 1:419121/69‑1 (MQ=255) gTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGGGTCTGACGTTGTGGCGTTtgtg < 1:2919634/69‑1 (MQ=255) gTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGGGTCTGACGTTGTGGCGTTtgtg < 1:1838177/69‑1 (MQ=255) gTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGGGTCTGACGTTGTGGCGTTtgtg < 1:1824811/69‑1 (MQ=255) gTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGGGTCTGACGTTGTGGCGTTtgtg < 1:3229247/69‑1 (MQ=255) gTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGGGTCTGACGTTGTGGCGTTtgtg < 1:3372142/69‑1 (MQ=255) gTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGGGTCTGACGTTGTGGCGTTtgtg < 1:2689269/69‑1 (MQ=255) gCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTTATATGTGTCTGACGTTGTGGCGTTTGTGaaa < 1:593004/69‑1 (MQ=255) gCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGaaa < 1:1069892/69‑1 (MQ=255) gCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGaaa < 1:3379978/69‑1 (MQ=255) gCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGaaa < 1:2560994/69‑1 (MQ=255) gCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGaaa < 1:1504062/69‑1 (MQ=255) gCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGaaa < 1:2250094/69‑1 (MQ=255) gCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGaaa < 1:1346119/69‑1 (MQ=255) cAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGaaa < 1:2356370/68‑1 (MQ=255) aaaaTATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTTTGAAac < 1:2935758/68‑1 (MQ=255) aaaaTATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAac < 1:2296990/68‑1 (MQ=255) aaaaTATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAac < 1:3280654/68‑1 (MQ=255) tatTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTgg > 1:661710/1‑69 (MQ=255) cGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTgg < 1:3006862/68‑1 (MQ=255) cGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTgg < 1:3230539/68‑1 (MQ=255) cGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTgg < 1:1817129/68‑1 (MQ=255) cGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTgg < 1:363044/68‑1 (MQ=255) cGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTgg < 1:1344697/68‑1 (MQ=255) cGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTgg < 1:2428438/68‑1 (MQ=255) cGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTgg < 1:3397328/68‑1 (MQ=255) gCACACCGCGTCCATTCAGGCTGATATGGGTCTGACGTTGTGGCGTTtgtg < 1:3224852/51‑1 (MQ=255) acCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAg < 1:492887/69‑1 (MQ=255) cgcgTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAg > 1:1473625/1‑67 (MQ=255) cgcgTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAg > 1:2843911/1‑67 (MQ=255) gcgTCCATTCAGGCTGATATGTGTCTGAGGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAg < 1:3207567/66‑1 (MQ=255) gcgTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAg < 1:758834/66‑1 (MQ=255) gcgTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAg < 1:685025/66‑1 (MQ=255) gcgTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAg < 1:624125/66‑1 (MQ=255) gcgTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAg < 1:962371/66‑1 (MQ=255) gcgTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAg < 1:1198181/66‑1 (MQ=255) gcgTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAg < 1:3341114/66‑1 (MQ=255) gcgTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAg < 1:2847987/66‑1 (MQ=255) gcgTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAg < 1:2546813/66‑1 (MQ=255) cgTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAg < 1:1387780/65‑1 (MQ=255) cgTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAg < 1:2647948/65‑1 (MQ=255) cgTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAg < 1:1962845/65‑1 (MQ=255) gTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGTCGGTGGCTTCCAg < 1:2425307/64‑1 (MQ=255) cATTCAGGCTGATATGGGTCTGACGTTGTGGCGTTtgtg < 1:384791/39‑1 (MQ=255) tCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAg > 1:3075111/1‑58 (MQ=255) tCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAg > 1:1909148/1‑58 (MQ=255) tCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAg > 1:2639192/1‑58 (MQ=255) tCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAg > 1:3428918/1‑58 (MQ=255) tCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAg > 1:2054799/1‑58 (MQ=255) tCAGGCTGATATGTGTCTGACGTTGTGGAGTTTGTGAAACACTGGCGGTGGCTTCCAg > 1:94034/1‑58 (MQ=255) acAGGCTGATATGTGTCTGAAGTTGTGGCGTTTGTGAAac > 1:2210016/2‑40 (MQ=39) cAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAGaa < 1:2108364/59‑1 (MQ=255) cAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAGaa < 1:2984832/59‑1 (MQ=255) cAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAGaa < 1:1817153/59‑1 (MQ=255) cAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAGaa < 1:871502/59‑1 (MQ=255) cAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAGaa < 1:876631/59‑1 (MQ=255) cAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAGaa < 1:348474/59‑1 (MQ=255) cAGGCTGATATGTGTCTGACGTCGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAGaa < 1:3113774/59‑1 (MQ=255) cTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAGAATGCCTTTTTCAGTa < 1:376820/69‑1 (MQ=255) tGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAGAATGCCTTTTTCAGTa < 1:1437120/68‑1 (MQ=255) tgtCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAGAATGCCTTTTTCAGTAAATGCa > 1:1443915/1‑67 (MQ=255) | AATTCAGGCCATCCCCACTGTTGCAAAATATTCACCGGCACACCGCGTCCATTCAGGCTGATATGTGTCTGACGTTGTGGCGTTTGTGAAACACTGGCGGTGGCTTCCAGAATGCCTTTTTCAGTAAATGCA > minE/2509495‑2509626 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |