| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,509,926 | C→T | 51.1% | P364P (CCG→CCA) | yicH ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,509,926 | 0 | C | T | 51.1% | ‑4.5 / 81.2 | 88 | P364P (CCG→CCA) | yicH | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (14/29); new base T (15/30); total (29/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTGCAGGCTGTTTAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCAACGTTTT > minE/2509868‑2509979 | gTTGCAGGCTGTTTAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTCGGCAAGGta < 1:3386592/68‑1 (MQ=255)gTTGCAGGCTGTTTAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTCGGCAAGGta < 1:462253/68‑1 (MQ=255) ttGCAGGCTGTTTAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTCGGCAAGGtata < 1:1707016/69‑1 (MQ=255) ttGCAGGCTGTTTAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTCGGCAAGGtata < 1:905848/69‑1 (MQ=255) ttGCAGGCTGTTTAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTCGGCAAGGtata < 1:2161375/69‑1 (MQ=255) ttGCAGGCTGTTTAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTCGGCAAGGtata < 1:1711040/69‑1 (MQ=255) ttGCAGGCTGTTTAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTCGGCAAGGtata < 1:1241828/69‑1 (MQ=255) ttGCAGGCTGTTTAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTCGGCAAGGtata < 1:460387/69‑1 (MQ=255) ttGCAGGCTGTTTAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTCGGCAAGGtata < 1:1958771/69‑1 (MQ=255) tttAACCAACCGGGTGTCGTTTCCATCCAGAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGtcc > 1:3084733/1‑68 (MQ=255) tttAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGtcc > 1:287777/1‑68 (MQ=255) tttAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGtcc > 1:3044067/1‑68 (MQ=255) tttAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGtcc > 1:3095057/1‑68 (MQ=255) tttAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGtcc > 1:2765476/1‑68 (MQ=255) tttAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGtcc > 1:2111874/1‑68 (MQ=255) tttAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGtcc > 1:2431010/1‑68 (MQ=255) tttAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGtcc > 1:2315441/1‑68 (MQ=255) tttAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGtcc > 1:2284408/1‑68 (MQ=255) tttAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGtcc > 1:1519562/1‑68 (MQ=255) tttAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGtcc > 1:132504/1‑68 (MQ=255) tttAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGtcc > 1:742069/1‑68 (MQ=255) tttAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGtcc > 1:835406/1‑68 (MQ=255) tttAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGtcc > 1:873557/1‑68 (MQ=255) tttAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGtcc > 1:94191/1‑68 (MQ=255) accaaccGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCa < 1:1290971/68‑1 (MQ=255) cGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAATTGcc < 1:1082017/61‑1 (MQ=255) cGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAATTGcc < 1:3239417/61‑1 (MQ=255) cGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAATTGcc < 1:3085564/61‑1 (MQ=255) cGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAATTGcc < 1:571287/61‑1 (MQ=255) cGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAATTGcc < 1:3032384/61‑1 (MQ=255) cGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAATTGcc < 1:639610/61‑1 (MQ=255) cGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAATTGcc < 1:2924485/61‑1 (MQ=255) cGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAATTGcc < 1:2539483/61‑1 (MQ=255) cGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAATTGcc < 1:763844/61‑1 (MQ=255) cGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAATTGcc < 1:1455071/61‑1 (MQ=255) cGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAATTGcc < 1:1477621/61‑1 (MQ=255) cGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAATTGcc < 1:206780/61‑1 (MQ=255) cGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAATTGcc < 1:1127129/61‑1 (MQ=255) cGTTTCCATCCACAACTGTTGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAATTGc < 1:2602243/60‑1 (MQ=255) gTTTCCATCCACAACTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCAtcatca < 1:2789965/69‑1 (MQ=255) tccatccaCAACTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAgg > 1:1212361/1‑69 (MQ=255) tccatccaCAACTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAgg > 1:1129709/1‑69 (MQ=255) tccaCAACTGTTGCCAGTTTTTCGGCCAGGTatat < 1:3122499/35‑1 (MQ=255) tccaCAACTGTTGCCAGTTTTTCGGCAAGGTttat < 1:1011083/35‑1 (MQ=255) tccaCAACTGTTGCCAGTTTTTCGGCAAGGTatat < 1:2515564/35‑1 (MQ=255) tccaCAACTGTTGCCAGTTTTTCGGCAAGGTatat < 1:600991/35‑1 (MQ=255) tccaCAACTGTTGCCAGTTTTTCGGCAAGGTatat < 1:211811/35‑1 (MQ=255) tccaCAACTGTTGCCAGTTTTTCGGCAAGGTatat < 1:202747/35‑1 (MQ=255) tccaCAACTGTTGCCAGTTTTTCGGCAAGGTatat < 1:857909/35‑1 (MQ=255) tccaCAACTGTTGCCAGTTTTTCGGCAAGGTatat < 1:543741/35‑1 (MQ=255) tccaCAACTGTTGCCAGTTTTTCGGCAAGGTatat < 1:2326083/35‑1 (MQ=255) ccaCAACTGTTGTCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCaa > 1:2705480/1‑69 (MQ=255) ccaCAACTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAATGATCaa > 1:498093/1‑69 (MQ=255) ccaCAACTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCaa > 1:1421643/1‑69 (MQ=255) ccaCAACTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCaa > 1:2690506/1‑69 (MQ=255) cacaACTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCAAc > 1:755192/1‑69 (MQ=255) cacaACTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCAAc > 1:550299/1‑69 (MQ=255) cacaACTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCAAc > 1:53286/1‑69 (MQ=255) cacaACTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCAAc > 1:2125555/1‑69 (MQ=255) cacaACTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCAAc > 1:2558819/1‑69 (MQ=255) cacaACTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCAAc > 1:1821019/1‑69 (MQ=255) cacaACTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCAAc > 1:802144/1‑69 (MQ=255) cacaACTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCAAc > 1:2006287/1‑69 (MQ=255) cTGTTGCCAGTTTTTCGGCAATGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCAACGtttt < 1:1105808/69‑1 (MQ=255) cTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCAACGtttt < 1:2405867/69‑1 (MQ=255) cTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCAACGtttt < 1:2258927/69‑1 (MQ=255) cTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCAACGtttt < 1:120531/69‑1 (MQ=255) cTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCAACGtttt < 1:692860/69‑1 (MQ=255) cTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCAACGtttt < 1:1848658/69‑1 (MQ=255) cTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCAACGtttt < 1:609806/69‑1 (MQ=255) tGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCAACGtttt < 1:162141/68‑1 (MQ=255) tGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCAACGtttt < 1:2505471/68‑1 (MQ=255) ttGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAAtt < 1:701157/40‑1 (MQ=37) ttGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAAtt < 1:93860/40‑1 (MQ=37) ttGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAAtt < 1:1644997/40‑1 (MQ=37) ttGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAAtt < 1:488363/40‑1 (MQ=37) ttGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAAtt < 1:1515185/40‑1 (MQ=37) ttGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAAtt < 1:362494/40‑1 (MQ=37) ttGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAAtt < 1:1651096/40‑1 (MQ=37) ttGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAAtt < 1:1726314/40‑1 (MQ=37) ttGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAAtt < 1:3040178/40‑1 (MQ=37) ttGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAAtt < 1:1928340/40‑1 (MQ=37) ttGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAAtt < 1:2922387/40‑1 (MQ=37) ttGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAAtt < 1:2665125/40‑1 (MQ=37) ttGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAAtt < 1:2075215/40‑1 (MQ=37) ttGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAAtt < 1:2427239/40‑1 (MQ=37) ttGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAAtt < 1:2210811/40‑1 (MQ=37) tGCCAGTTTTTTGGCAAGGTATATTCCAGTCCGGCAAtt < 1:908100/39‑1 (MQ=37) | GTTGCAGGCTGTTTAACCAACCGGGTGTCGTTTCCATCCACAACTGTTGCCAGTTTTTCGGCAAGGTATATTCCAGCCCGGCAATTGCCGCATCATCAAGGATCAACGTTTT > minE/2509868‑2509979 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |