| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 214,077 | C→A | 27.0% | T146T (ACC→ACA) | yafS → | predicted S‑adenosyl‑L‑methionine‑dependent methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 214,077 | 0 | C | A | 27.0% | 70.5 / 21.9 | 48 | T146T (ACC→ACA) | yafS | predicted S‑adenosyl‑L‑methionine‑dependent methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (13/22); new base A (3/10); total (16/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
TAGTGGCTTCAATCCCATCAGTTTTATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTT > minE/214014‑214142 | tAGTGGCTTCAATCCCATCAGTTTTATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGc < 1:3106568/68‑1 (MQ=255) aGTGGCTTCAATCCCATCAGTTTTATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGccc > 1:246945/1‑69 (MQ=255) aGTGGCTTCAATCCCATCAGTTTTATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGccc > 1:3325202/1‑69 (MQ=255) gCTTCAATCCCATCAGTTTTATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAATCCTCGCCCtat > 1:2568948/1‑68 (MQ=255) gCTTCAATCCCATCAGTTTTATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGcc < 1:397752/64‑1 (MQ=255) gCTTCAATCCCATCAGTTTTATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCtat > 1:2514039/1‑68 (MQ=255) gCTTCAATCCCATCAGTTTTATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCtat > 1:2055209/1‑68 (MQ=255) cAATCCCATCAGTTTAATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAg > 1:2107394/1‑69 (MQ=255) cAATCCCATCAGTTTAATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAg > 1:171163/1‑69 (MQ=255) cAATCCCATCAGTTTAATGGGATTACGCAAACTTGTGCCGGTATTGAGCAAAACATCGCGCTATAACa > 1:2583379/1‑68 (MQ=255) tCCCATCAGTTTTATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCg < 1:3172292/69‑1 (MQ=255) tCCCATCAGTTTTATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCg < 1:2418470/69‑1 (MQ=255) tCCCATCAGTTTTATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCg < 1:3228889/69‑1 (MQ=255) tttATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTAct > 1:101516/1‑69 (MQ=255) tttATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTAct > 1:1154519/1‑69 (MQ=255) tttATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTAct > 1:1191634/1‑69 (MQ=255) aTTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGc < 1:3335259/68‑1 (MQ=255) aTTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGc < 1:2668743/68‑1 (MQ=255) aTTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGc < 1:1391254/68‑1 (MQ=255) aTTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGc < 1:1403562/68‑1 (MQ=255) aTTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGc < 1:768947/68‑1 (MQ=255) tACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGc < 1:2505278/69‑1 (MQ=255) tACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGc < 1:2544681/69‑1 (MQ=255) aCGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAGCCGGATGTTCACTCTGATGCGGCa < 1:843974/69‑1 (MQ=255) aCGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAGCCGGATGTTCACTCTGATGCGGCa < 1:3302819/69‑1 (MQ=255) aCGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAGCCGGATGTTCACTCTGATGCGGCa < 1:2718424/69‑1 (MQ=255) aCGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAGCCGGATGTTCACTCTGATGCGGCa < 1:923972/69‑1 (MQ=255) aCGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAGCCGGATGTTCACTCTGATGCGGCa < 1:2817348/69‑1 (MQ=255) aCGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAGCCGGATGTTCACTCTGATGCGGCa < 1:2813443/69‑1 (MQ=255) aCGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAGCCGGATGTTCACTCTGATGCGGCa < 1:2511165/69‑1 (MQ=255) aCGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAGCCGGATGTTCACTCTGATGCGGCa < 1:1613839/69‑1 (MQ=255) aCGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAGCCGGATGTTCACTCTGATGCGGCa < 1:2116587/69‑1 (MQ=255) aCGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAGCCGGATGTTCACTCTGATGCGGCa < 1:1743026/69‑1 (MQ=255) aaaCTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGc < 1:2954137/61‑1 (MQ=255) aaCTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTgg < 1:1531834/69‑1 (MQ=255) tATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCtt < 1:1096206/69‑1 (MQ=255) tATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCCCTCtt < 1:867913/69‑1 (MQ=255) ttGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTttg > 1:330919/1‑69 (MQ=255) gcgcAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTg < 1:3307413/52‑1 (MQ=255) gcgcAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTg < 1:3252395/52‑1 (MQ=255) gcgcAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTg < 1:71862/52‑1 (MQ=255) gcgcAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTg < 1:3066190/52‑1 (MQ=255) gcgcAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTg < 1:1219454/52‑1 (MQ=255) gcgcAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTg < 1:2979406/52‑1 (MQ=255) aCCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAAttt > 1:1952292/1‑68 (MQ=255) aCCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAAttt > 1:498166/1‑68 (MQ=255) aCCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAAttt > 1:715271/1‑68 (MQ=255) aCCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAAttt > 1:302534/1‑68 (MQ=255) | TAGTGGCTTCAATCCCATCAGTTTTATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTT > minE/214014‑214142 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |