| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,514,597 | G→A | 41.3% | G556G (GGC→GGT) | recG ← | ATP‑dependent DNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,514,597 | 0 | G | A | 41.3% | 42.2 / 74.7 | 104 | G556G (GGC→GGT) | recG | ATP‑dependent DNA helicase |
| Reads supporting (aligned to +/- strand): ref base G (28/33); new base A (18/25); total (46/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
TAACTGCGCCAGACCCAGACGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAACGACGCCATCACCGC > minE/2514531‑2514665 | tAACTGCGCCAGACCCAGACGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGcc < 1:80186/69‑1 (MQ=255)tAACTGCGCCAGACCCAGACGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGcc > 1:769063/1‑69 (MQ=255)tAACTGCGCCAGACCCAGACGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGcc < 1:1777714/69‑1 (MQ=255)tAACTGCGCCAGACCCAGACGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGcc > 1:2012991/1‑69 (MQ=255)tAACTGCGCCAGACCCAGACGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGcc > 1:2220974/1‑69 (MQ=255)tAACTGCGCCAGACCCAGACGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGcc < 1:2256756/69‑1 (MQ=255)tAACTGCGCCAGACCCAGACGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGcc > 1:3266814/1‑69 (MQ=255)tAACTGCGCCAGACCCAGACGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGcc > 1:2812299/1‑69 (MQ=255)tAACTGCGCCAGACCCAGACGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGcc < 1:3192899/69‑1 (MQ=255)tAACTGCGCCAGACCCAGACGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGcc > 1:879179/1‑69 (MQ=255)tAACTGCGCCAGACCCAGACGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGcc > 1:296816/1‑69 (MQ=255)tAACTGCGCCAGACCCAGACGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGcc < 1:921718/69‑1 (MQ=255)tAACTGCGCCAGACCCAGACGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGcc > 1:1011731/1‑69 (MQ=255)tAACTGCGCCAGACCCAGACGCTCCGGGTTTTCGATAATCAT‑AGACTGGCGTTAGGCACATCAACGCCg > 1:2954117/1‑69 (MQ=255) aaCTGCGCCAGACCCAGACGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGcc < 1:2705857/68‑1 (MQ=255) aaCTGCGCCAGACCCAGACGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGcc < 1:3296166/68‑1 (MQ=255) aaCTGCGCCAGACCCAGACGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGcc < 1:1018653/68‑1 (MQ=255) cagacccagacGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAAt < 1:1129149/69‑1 (MQ=255) cagacccagacGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAAt < 1:2197133/69‑1 (MQ=255) cagacccagacGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAAt < 1:2183784/69‑1 (MQ=255) cagacccagacGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAAt < 1:1068086/69‑1 (MQ=255) cagacccagacGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAAt < 1:1678317/69‑1 (MQ=255) tCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCa < 1:2996902/69‑1 (MQ=255) tCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCa < 1:54155/69‑1 (MQ=255) tCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCa < 1:614129/69‑1 (MQ=255) tCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCa < 1:1515328/69‑1 (MQ=255) tCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCa < 1:1457361/69‑1 (MQ=255) gTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACcag < 1:3103115/69‑1 (MQ=255) gTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACcag < 1:2770274/69‑1 (MQ=255) gTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACcag < 1:436851/69‑1 (MQ=255) gTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACcag < 1:1606735/69‑1 (MQ=255) ttCGATAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGt < 1:1220642/54‑1 (MQ=255) ttCGATAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGt < 1:549214/54‑1 (MQ=255) ttCGATAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGt < 1:2134221/54‑1 (MQ=255) cGATAATCATCAGACTGGCGTTAGGCACATCAACGcc < 1:3078967/37‑1 (MQ=255) gATAATCATCAGACTGGCGGTAGGCACATCAACGCCGACTTCAAt < 1:2195404/45‑1 (MQ=255) tAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGgtgt < 1:2128540/68‑1 (MQ=255) tAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGgtgt < 1:43133/68‑1 (MQ=255) tAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGgtgt < 1:2608189/68‑1 (MQ=255) tAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGgtgt < 1:2316055/68‑1 (MQ=255) aaTCATCAGACTGGGGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:1993005/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGTCACATCAACACCGACTTCAATAACGGtag < 1:1861983/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:1576716/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:882937/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:782423/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:2945623/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:73290/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:1899270/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:81118/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:1258622/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:278865/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:653986/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:1277835/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:1355578/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:2669736/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:329277/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:1597566/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:2597497/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:1421200/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:2085045/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:1579426/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:791456/50‑3 (MQ=255) aaTCATCAGACTGGCGTTAGGCACATCAACACCGACTTCAATAACGGtag < 1:2255798/50‑3 (MQ=255) atcaGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCa > 1:944491/1‑69 (MQ=255) aGACTGGCGTTAGGCCCATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCt > 1:3172694/1‑69 (MQ=255) aGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCt > 1:2877837/1‑69 (MQ=255) aGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCt > 1:2873913/1‑69 (MQ=255) aGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCt > 1:2585643/1‑69 (MQ=255) aGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCt > 1:1577278/1‑69 (MQ=255) aGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCt > 1:1967582/1‑69 (MQ=255) aGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAacga > 1:2205856/1‑69 (MQ=255) aGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAacga > 1:458976/1‑69 (MQ=255) aGGCACATCAACGCCGACTTCAATAACGGTTGTGG‑AACCAGCAGGTGTAGCTCACCTTGTTTAAacgac > 1:203564/1‑69 (MQ=255) caTCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAACGACGcc > 1:2323382/1‑68 (MQ=255) tCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAACGACGCCATc > 1:1342670/1‑69 (MQ=255) tCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAACGACGCCATc > 1:1237275/1‑69 (MQ=255) tCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAACGACGCCATc > 1:1146665/1‑69 (MQ=255) tCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAACGACGCCATc > 1:905446/1‑69 (MQ=255) tCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAACGACGCCATc > 1:2846337/1‑69 (MQ=255) tCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCCTGTTTAAACGACGCCATc > 1:3217944/1‑69 (MQ=255) cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa > 1:1077283/1‑56 (MQ=255) cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa > 1:1715280/1‑56 (MQ=255) cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa > 1:850220/1‑56 (MQ=255) cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa > 1:852239/1‑56 (MQ=255) cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa > 1:856969/1‑56 (MQ=255) cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa > 1:1104387/1‑56 (MQ=255) cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa > 1:764688/1‑56 (MQ=255) cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa > 1:186713/1‑56 (MQ=255) cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa > 1:71997/1‑56 (MQ=255) cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa > 1:598103/1‑56 (MQ=255) cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa > 1:2183273/1‑56 (MQ=255) cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa > 1:535066/1‑56 (MQ=255) cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa > 1:2424070/1‑56 (MQ=255) cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa > 1:414900/1‑56 (MQ=255) cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa > 1:3370996/1‑56 (MQ=255) cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa > 1:2662940/1‑56 (MQ=255) cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGATc > 1:1618887/1‑46 (MQ=255) cAACACCGACTTCAATAAAGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa > 1:3093716/1‑56 (MQ=255) gCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAACGACGCCATCACCGc > 1:1194439/1‑69 (MQ=255) gCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAACGACGCCATCACCGc > 1:272011/1‑69 (MQ=255) | TAACTGCGCCAGACCCAGACGCTCCGGGTTTTCGATAATCATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAACGACGCCATCACCGC > minE/2514531‑2514665 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |