Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,514,636 C→T 54.3% E543E (GAG→GAA recG ← ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,514,6360CT54.3% 15.0 / 97.6 115E543E (GAG→GAArecGATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base C (47/5);  new base T (17/45);  total (65/50)
Fisher's exact test for biased strand distribution p-value = 4.98e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

ATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTCTCGGCAGGTTTCATCCGCCCGTGTACCAGG  >  minE/2514571‑2514702
                                                                 |                                                                  
atcaGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCa                                                                 >  1:944491/1‑69 (MQ=255)
atcaGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCa                                                                 >  1:3167162/1‑69 (MQ=255)
   aGACTGGCGTTAGGCCCATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCt                                                              >  1:3172694/1‑69 (MQ=255)
   aGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCt                                                              >  1:980550/1‑69 (MQ=255)
   aGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCt                                                              >  1:2585643/1‑69 (MQ=255)
   aGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCt                                                              >  1:2873913/1‑69 (MQ=255)
   aGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCt                                                              >  1:2877837/1‑69 (MQ=255)
   aGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCt                                                              >  1:1967582/1‑69 (MQ=255)
   aGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCt                                                              >  1:1577278/1‑69 (MQ=255)
              aGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAacga                                                   >  1:2205856/1‑69 (MQ=255)
              aGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAacga                                                   >  1:458976/1‑69 (MQ=255)
              aGGCACATCAACGCCGACTTCAATAACGGTTGTGGAACCAGCAGGTGTAGCTCACCTTGTTTAAacgac                                                  >  1:203564/1‑69 (MQ=255)
                   caTCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAACGACGcc                                               >  1:2323382/1‑68 (MQ=255)
                     tCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAACGACGCCATc                                            >  1:905446/1‑69 (MQ=255)
                     tCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAACGACGCCATc                                            >  1:1342670/1‑69 (MQ=255)
                     tCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAACGACGCCATc                                            >  1:1146665/1‑69 (MQ=255)
                     tCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAACGACGCCATc                                            >  1:2846337/1‑69 (MQ=255)
                     tCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAACGACGCCATc                                            >  1:1237275/1‑69 (MQ=255)
                      cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa                                                        >  1:1715280/1‑56 (MQ=255)
                      cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa                                                        >  1:2183273/1‑56 (MQ=255)
                      cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa                                                        >  1:3370996/1‑56 (MQ=255)
                      cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa                                                        >  1:1104387/1‑56 (MQ=255)
                      cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa                                                        >  1:186713/1‑56 (MQ=255)
                      cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa                                                        >  1:414900/1‑56 (MQ=255)
                      cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa                                                        >  1:1077283/1‑56 (MQ=255)
                      cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa                                                        >  1:2424070/1‑56 (MQ=255)
                      cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa                                                        >  1:535066/1‑56 (MQ=255)
                      cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa                                                        >  1:71997/1‑56 (MQ=255)
                      cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa                                                        >  1:856969/1‑56 (MQ=255)
                      cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa                                                        >  1:2662940/1‑56 (MQ=255)
                      cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa                                                        >  1:764688/1‑56 (MQ=255)
                      cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa                                                        >  1:850220/1‑56 (MQ=255)
                      cAACACCGACTTCAATAACGGTAGTGGCAACCAGCAGGTGTAGATc                                                                  >  1:1618887/1‑46 (MQ=255)
                      cAACACCGACTTCAATAAAGGTAGTGGCAACCAGCAGGTGTAGTTCACCTTGTTTa                                                        >  1:3093716/1‑56 (MQ=255)
                                 tCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTa                                                        >  1:898020/1‑45 (MQ=255)
                                 tCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTa                                                        >  1:279948/1‑45 (MQ=255)
                                 tCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTa                                                        >  1:2032729/1‑45 (MQ=255)
                                 tCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTa                                                        >  1:3395552/1‑45 (MQ=255)
                                 tCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTa                                                        >  1:1781081/1‑45 (MQ=255)
                                 tCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTa                                                        >  1:1400682/1‑45 (MQ=255)
                                 tCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTa                                                        >  1:1396485/1‑45 (MQ=255)
                                 tCAATAACGGTTGTGGAAACCAGCAGGTGTAGCTCACCTTGTTTa                                                        >  1:3384120/1‑45 (MQ=255)
                                    aTAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAACGACGCCATCACCGCCTGTTtctc                              >  1:1514964/1‑68 (MQ=255)
                                             gtgGCAACCAGCAGGTGTAGCTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTCTCGGCAGGTTTc                    <  1:2160871/69‑1 (MQ=255)
                                                     cagcagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGccc            <  1:3144080/69‑1 (MQ=255)
                                                     cagcagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGccc            <  1:3362210/69‑1 (MQ=255)
                                                     cagcagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGccc            <  1:304673/69‑1 (MQ=255)
                                                     cagcagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGccc            <  1:472922/69‑1 (MQ=255)
                                                     cagcagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGccc            <  1:2859455/69‑1 (MQ=255)
                                                     cagcagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGccc            <  1:2822737/69‑1 (MQ=255)
                                                     cagcagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGccc            <  1:2646332/69‑1 (MQ=255)
                                                     cagcagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGccc            <  1:1065353/69‑1 (MQ=255)
                                                     cagcagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGccc            <  1:1914087/69‑1 (MQ=255)
                                                     cagcagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGccc            <  1:1324770/69‑1 (MQ=255)
                                                     cagcagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGccc            <  1:2470139/69‑1 (MQ=255)
                                                     cagcagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGccc            <  1:2296923/69‑1 (MQ=255)
                                                     cagcagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGccc            <  1:1796638/69‑1 (MQ=255)
                                                     cagcagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGccc            <  1:2251705/69‑1 (MQ=255)
                                                     cagcagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGccc            <  1:1706930/69‑1 (MQ=255)
                                                      atcagGTGTAGCTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTCTCGGCAGGTTTCATCCGCCCg           >  1:3225559/3‑69 (MQ=255)
                                                      agcagGTGTGGCTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTCTCGGCAGGTTTCATCCGCCCg           >  1:2437335/1‑69 (MQ=255)
                                                      agcagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGccc            <  1:451180/68‑1 (MQ=255)
                                                      agcagGTGTAGCTCACCTTGTTTAAACGCCGCCATCACCGCCTGTTTCTCGGCAGGTTTCATCCGCCCg           >  1:3014820/1‑69 (MQ=255)
                                                      agcagGTGTAGCTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTCTCGGCAGGTTTCATCCGCCCg           >  1:596321/1‑69 (MQ=255)
                                                      agcagGTGTAGCTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTCTCGGCAGGTTTCATCCGCCCg           >  1:1812029/1‑69 (MQ=255)
                                                      agcagGTGTAGCTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTCTCGGCAGGTTTCATCCGCCCg           >  1:1699811/1‑69 (MQ=255)
                                                      agcagGTGTAGCTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTCTCGGCAGGTTTCATCCGCCCg           <  1:2498762/69‑1 (MQ=255)
                                                      agcagGTGTAGCTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTCTCGGCAGGTTTCATCCGCCCg           >  1:659513/1‑69 (MQ=255)
                                                      agcagGTGTAGCTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTCTCGGCAGGTTTCATCCGCCCg           >  1:826749/1‑69 (MQ=255)
                                                      agcagGTGTAGCTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTCTCGGCAGGTTTCATCCGCCCg           >  1:1001879/1‑69 (MQ=255)
                                                      agcagGTGTAGCTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTCTCGGCAGGTTTCATCCGCCCg           >  1:2347165/1‑69 (MQ=255)
                                                      agcagGTGTAGCTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTCTCGGCAGGTTTCATCCGCCCg           >  1:2066149/1‑69 (MQ=255)
                                                      agcagGTGTAGCTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTCTCGGCAGGTTTCATCCGCCCg           >  1:2482183/1‑69 (MQ=255)
                                                      agcagGTGTAGCTCACCTTGTTTAAACGACGCCATCACCGCCTCTTTCTCGGCAGGTTTCATCCGCCCg           >  1:1900228/1‑69 (MQ=255)
                                                      agcagGTGTAGCTCAACTTGTTTAAACGACGCCATCACCGCCTGTTTCTCGGCAGGTTTCATCCGCCCg           >  1:3306913/1‑69 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAAGGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:3324736/69‑1 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:1116313/69‑1 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:636629/69‑1 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:673688/69‑1 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:1341912/69‑1 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:2433313/69‑1 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:1211831/69‑1 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:1207817/69‑1 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:54825/69‑1 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:1098157/69‑1 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:962175/69‑1 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:2573470/69‑1 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:3036263/69‑1 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:49827/69‑1 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:497088/69‑1 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:488318/69‑1 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:2231401/69‑1 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:2184441/69‑1 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:2176690/69‑1 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:344109/69‑1 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:1932451/69‑1 (MQ=255)
                                                        cagGTGTAGTTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTATCGGCAGGTTTCATCCGCCCgtg         <  1:335399/69‑1 (MQ=255)
                                                           gtgtAGCTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTCTCGGCAGGTTTCATCCGCCCGTGTAc      <  1:1667735/69‑1 (MQ=255)
                                                           gtgtAGCTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTCTCGGCAGGTTTCATCCGCCCGTGTAc      <  1:161819/69‑1 (MQ=255)
                                                                gCTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTCTCGGCAGGTTTCATCCGCCCGTGTACCAgg  >  1:3332250/1‑68 (MQ=255)
                                                                 |                                                                  
ATCAGACTGGCGTTAGGCACATCAACGCCGACTTCAATAACGGTTGTGGCAACCAGCAGGTGTAGCTCACCTTGTTTAAACGACGCCATCACCGCCTGTTTCTCGGCAGGTTTCATCCGCCCGTGTACCAGG  >  minE/2514571‑2514702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: