| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,517,413 | G→A | 54.0% | S554S (AGC→AGT) | spoT ← | bifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,517,413 | 0 | G | A | 54.0% | 5.6 / 61.8 | 74 | S554S (AGC→AGT) | spoT | bifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Reads supporting (aligned to +/- strand): ref base G (19/15); new base A (30/10); total (49/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.23e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
GGGCGGCAGCATTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCTTTGGGTTGCCGGTGGAATGGAGGCGTCCCCATGTTGCAGATTTTTCGCGACC > minE/2517348‑2517467 | gggCGGCAGCATTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCtt > 1:1012069/1‑69 (MQ=255)gggCGGCAGCATTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCtt > 1:2544599/1‑69 (MQ=255)gggCGGCAGCATTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCtt > 1:2661301/1‑69 (MQ=255)gggCGGCAGCATTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCtt > 1:3086987/1‑69 (MQ=255)gggCGGCAGCATTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCtt > 1:3288902/1‑69 (MQ=255)gggCGGCAGCATTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCtt > 1:3325711/1‑69 (MQ=255)gggCGGCAGCATTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCtt > 1:599380/1‑69 (MQ=255)gggCGGCAGCATTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCtt > 1:106636/1‑69 (MQ=255)gggCGGCAGCATTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTAAATGGGCAGATGTCCGTGGCt > 1:2951968/1‑68 (MQ=255)gggCGGCAGCATTTCGCAAAGGTGATCAGCACGCCATCGGCACC‑TTAATGGGCAGATGTCCGTGGCttt > 1:1920162/1‑69 (MQ=255) cggcAGCATTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCTTTgg < 1:716167/69‑1 (MQ=255) cggcAGCATTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCTTTgg < 1:793647/69‑1 (MQ=255) caTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCTTTGGGTTg > 1:3336207/1‑67 (MQ=255) caTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCTTTGGGTTGcc > 1:3401967/1‑69 (MQ=255) caTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCTTTGGGTTGcc > 1:714513/1‑69 (MQ=255) caTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCTTTGGGTTGcc > 1:2711124/1‑69 (MQ=255) caTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCTTTGGGTTGcc > 1:1589771/1‑69 (MQ=255) aTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACttt > 1:2141138/1‑60 (MQ=255) aTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTggg > 1:515424/1‑63 (MQ=255) aTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTgg > 1:1052157/1‑62 (MQ=255) aTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:1574396/1‑69 (MQ=255) aTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:946309/1‑69 (MQ=255) aTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:929547/1‑69 (MQ=255) aTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:851254/1‑69 (MQ=255) aTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:786459/1‑69 (MQ=255) aTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:11442/1‑69 (MQ=255) aTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:1547347/1‑69 (MQ=255) aTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:3058103/1‑69 (MQ=255) aTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:2566891/1‑69 (MQ=255) aTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:1610349/1‑69 (MQ=255) aTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:2454573/1‑69 (MQ=255) aTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:2406829/1‑69 (MQ=255) aTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:1710278/1‑69 (MQ=255) aTTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:2096886/1‑69 (MQ=255) aTTTCGAAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:7940/1‑69 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCTTTGGGTTGCCGGTgg < 1:1554541/68‑1 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCTTTGGGTTGCCGGTgg < 1:3074807/68‑1 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCTTTGGGTTGCCGGTgg < 1:2147246/68‑1 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCTTTGGGTTGCCGGTgg < 1:2297241/68‑1 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCTTTGGGTTGCCGGTgg < 1:1766372/68‑1 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCTTTGGGTTGCCGGTgg < 1:2898425/68‑1 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCTTTGGGTTGCCGGTgg < 1:1586066/68‑1 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCTTTGGGTTGCCGGTgg < 1:2220079/68‑1 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCTTTGGGTTGCCGGTgg < 1:2013780/68‑1 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:1679638/1‑64 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:1285066/1‑64 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:1133073/1‑64 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:1063222/1‑64 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:1319310/1‑64 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:1059273/1‑64 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:801084/1‑64 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:3211537/1‑64 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:2998619/1‑64 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:2276592/1‑64 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:2532687/1‑64 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGACTTTGGGTTGCCg > 1:1603584/1‑64 (MQ=255) gCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATCTCCGTGGCTTTGGGTTGCCGGTgg < 1:115823/68‑1 (MQ=255) cAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCTTTGGGTTGCCGGTgg < 1:2632356/67‑1 (MQ=255) ttAATGGGCAGATGTCCGTGGCTTTGGGTTGCCGGTGGAATGGAGGCGTCCCCATGTTGCAGATTTTTc > 1:199279/1‑69 (MQ=255) aTGGGCTGATGTCCGTGGCTTTGGGTTGCCGGTGGAATGGAGGCGTCCCCATGTTGCAGATTTTTcgcg > 1:438881/1‑69 (MQ=255) aTGGGCAGATGTCCGTGGCTTTGGGTTGCCGGTGGAATGGAGGCGTCCCCATGTTGCAGATTTTTcgcg > 1:750983/1‑69 (MQ=255) gggCAGATGTCCGTGGCTTTGGGTTGCCGGTGGAATGGAGGCGTCCCCATGTTGCAGATTTTTcgcg < 1:860059/67‑1 (MQ=255) gggCAGATGTCCGTGGCTTTGGGTTGCCGGTGGAATGGAGGCGTCCCCATGTTGCAGATTTTTcgcg < 1:2555704/67‑1 (MQ=255) ggCAGATGTCCGTGGCTTTGGGTTGCCGGTGGAATGGAGGCGTCCCCATGTTGCAGATTTTTCGCGacc > 1:2508364/1‑69 (MQ=255) ccGTGACTTTGGGTTGCCGGTGGAATGGAGGCGTcccc < 1:752663/38‑1 (MQ=255) ccGTGACTTTGGGTTGCCGGTGGAATGGAGGCGTcccc < 1:250349/38‑1 (MQ=255) ccGTGACTTTGGGTTGCCGGTGGAATGGAGGCGTcccc < 1:214430/38‑1 (MQ=255) ccGTGACTTTGGGTTGCCGGTGGAATGGAGGCGTcccc < 1:2553893/38‑1 (MQ=255) ccGTGACTTTGGGTTGCCGGTGGAATGGAGGCGTcccc < 1:2688500/38‑1 (MQ=255) ccGTGACTTTGGGTTGCCGGTGGAATGGAGGCGTcccc < 1:2185478/38‑1 (MQ=255) ccGTGACTTTGGGTTGCCGGTGGAATGGAGGCGTcccc < 1:1068665/38‑1 (MQ=255) ccGTGACTTTGGGTTGCCGGTGGAATGGAGGCGTcccc < 1:559003/38‑1 (MQ=255) ccGTGACTTTGGGTTGCCGGTGGAATGGAGGCGTcccc < 1:294089/38‑1 (MQ=255) cGTGACTTTGGGTTGCCGGTGGAATGGAGGCGTcccc < 1:3143207/37‑1 (MQ=255) | GGGCGGCAGCATTTCGCAAAGGTGATCAGCACGCCATCGGCACCTTTAATGGGCAGATGTCCGTGGCTTTGGGTTGCCGGTGGAATGGAGGCGTCCCCATGTTGCAGATTTTTCGCGACC > minE/2517348‑2517467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |