| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,517,491 | T→G | 67.9% | G528G (GGA→GGC) | spoT ← | bifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,517,491 | 0 | T | G | 67.9% | 86.4 / 43.4 | 73 | G528G (GGA→GGC) | spoT | bifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Reads supporting (aligned to +/- strand): ref base T (9/14); new base G (17/32); total (27/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01 | |||||||||||
GAGGCGTCCCCATGTTGCAGATTTTTCGCGACCACCACGCTCATTGCGTTACCAAGTCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAGCTTCATGCGATCCAGCTCGCGCTGAATATT > minE/2517435‑2517556 | gAGGCGTCCCCATGTTGCAGATTTTTCGCGACCACCACGCTCATTGCGTTACCAAGTCCGATTTCTGcc > 1:2023797/1‑69 (MQ=255)gAGGCGTCCCCATGTTGCAGATTTTTCGCGACCACCACGCTCATTGCGTTACCAAGTCCGATTTCTGcc > 1:1099419/1‑69 (MQ=255)gAGGCGTCCCCATGTTGCAGATTTTTCGCGACCACCACGCTCATTGCGTTACCAAGTCCGATTTCTGcc > 1:1736600/1‑69 (MQ=255) gTCCCCATGTTGCAGATTTTTCGCGACCACCACGCTCATTGCGTTACCAAGTCCGATTTCTGCcagcag < 1:2390680/69‑1 (MQ=255) gTCCCCATGTTGCAGATTTTTCGCGACCACCACGCTCATTGCGTTACCAAGTCCGATTTCTGCcagcag < 1:1668686/69‑1 (MQ=255) ttttCGCGACCACCACGCTCATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGCGTTGcc > 1:726176/1‑68 (MQ=255) ttttCGCGACCACCACGCTCATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGCGTTGcc > 1:3268308/1‑68 (MQ=255) ttttCGCGACCACCACGCTCATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGCGTTGcc > 1:2517105/1‑68 (MQ=255) ttttCGCGACCACCACGCTCATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGCGTTGcc > 1:2599092/1‑68 (MQ=255) ttttCGCGACCACCACGCTCATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGCGTTGcc > 1:361927/1‑68 (MQ=255) ttttCGCGACCACCACGCTCATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGCGTTGcc > 1:2866766/1‑68 (MQ=255) ttttCGCGACCACCACGCTCATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGCGTTGcc > 1:1960459/1‑68 (MQ=255) ttttCGCGACCACCACGCTCATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGCGTTGcc > 1:3190186/1‑68 (MQ=255) ttttCGCGACCACCACGCTCATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGCGTTGcc > 1:1739639/1‑68 (MQ=255) ttttCGCGACCACCACGCTCATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGCGTTGcc > 1:1174208/1‑68 (MQ=255) ttttCGCGACCACCACGCTCATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGCGTTGcc > 1:1685842/1‑68 (MQ=255) ttttCGCGACCACCACGCTCATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGCGTTGcc > 1:667565/1‑68 (MQ=255) ttttCGCGACCACCACGCTCATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGCGTTGcc > 1:1692231/1‑68 (MQ=255) tttCGCGACCACCACGCTCATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAg > 1:604463/1‑69 (MQ=255) tttCGCGACCACCACGCTCATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAg > 1:460637/1‑69 (MQ=255) tttCGCGACCACCACGCTCATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAg > 1:1510973/1‑69 (MQ=255) tttCGCGACCACCACGCTCATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAg > 1:1528791/1‑69 (MQ=255) gcgACCACCACGCTCATTGCGTTACCAAGTCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAGCtt < 1:1517555/68‑1 (MQ=255) gcgACCACCACGCTCATTGCGTTACCAAGTCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAGCtt < 1:810121/68‑1 (MQ=255) gcgACCACCACGCTCATTGCGTTACCAAGTCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAGCtt < 1:3303132/68‑1 (MQ=255) gcgACCACCACGCTCATTGCGTTACCAAGTCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAGCtt < 1:2777303/68‑1 (MQ=255) cgACCACCACGCTCATTGCGTTACCAAGTCCGATTTCTGCCAGCAGATCGTCAAGCGTTGTCAGCtt < 1:440513/67‑1 (MQ=255) gCTCATTGCGTTACCAAGTCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAGCTTTATGCGATCCAg < 1:3047077/69‑1 (MQ=255) gCTCATTGCGTTACCAAGTCCGATTTCTGCCAGCAGATCGTCAAGCGCTGCCAGCTTCATGCGATCCAg < 1:1397103/69‑1 (MQ=255) cTCATTGCGTTACCAAGTCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAGc > 1:2309665/1‑54 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:3259361/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:2933767/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:453801/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:3295298/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:3370405/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:3427638/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:2670984/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:546028/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:613921/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:816858/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:835266/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:902264/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:1895517/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:1112990/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:1113055/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:1284374/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:1506666/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:1605107/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:174115/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:1788711/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:1874294/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:286420/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:2042921/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:2153608/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:231766/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:2848815/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:2733687/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:2703828/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:2356614/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:2352620/43‑1 (MQ=255) cATTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:2313718/43‑1 (MQ=255) aTTGCGTTACCAAGGCCGATTTCTGCCAGCAGATCGTCAAGc < 1:625709/42‑1 (MQ=255) gCGTTACCAAGTCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAGCTTCATGCGATCCAGCTcgcg > 1:1831067/1‑68 (MQ=255) aCCAAGTCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAGCTTCATGCGATCCAGCTCGCGCTGAat > 1:2508321/1‑69 (MQ=255) aCCAAGTCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAGCTTCATGCGATCCAGCTCGCGCTGAat > 1:3138113/1‑69 (MQ=255) aCCAAGTCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAGCTTCATGCGATCCAGCTCGCGCTGAat > 1:1186609/1‑69 (MQ=255) aCCAAGACCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAGCTTCATGCGATCCAGCTCGCGCTGaa > 1:550/1‑68 (MQ=255) cAAGTCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAGCTTCATGCGATCCAGCTCGCGCTGAata < 1:571252/68‑1 (MQ=255) aaGTCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAGCTTCATGCGATCCAGCTCGCGCTGAATAtt < 1:3242368/69‑1 (MQ=255) aaGTCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAGCTTCATGCGATCCAGCTCGCGCTGAATAtt > 1:2672277/1‑69 (MQ=255) aaGTCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAGCTTCATGCGATCCAGCTCGCGCTGAATAtt < 1:621183/69‑1 (MQ=255) aaGTCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAGCTTCATGCGATCCAGCTCGCGCTGAATAtt < 1:3087370/69‑1 (MQ=255) aaGTCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAGCTTCATGCGATCCAGCTCGCGCTGAATAtt < 1:1177740/69‑1 (MQ=255) | GAGGCGTCCCCATGTTGCAGATTTTTCGCGACCACCACGCTCATTGCGTTACCAAGTCCGATTTCTGCCAGCAGATCGTCAAGCGTTGCCAGCTTCATGCGATCCAGCTCGCGCTGAATATT > minE/2517435‑2517556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |