| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,517,725 | G→A | 64.3% | G450G (GGC→GGT) | spoT ← | bifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,517,725 | 0 | G | A | 64.3% | 22.9 / 22.3 | 42 | G450G (GGC→GGT) | spoT | bifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Reads supporting (aligned to +/- strand): ref base G (9/6); new base A (18/9); total (27/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01 | |||||||||||
GCGCGCGCTTTCGAGCTAACGACAAAGTTCAGCCAAGCGGCATTCGGGCGAGCGCCCGGAGCGGTAATGATTTCAACGGTTTGACCGCTGGTAAGCGGCTGCGACAGCGGGTA > minE/2517672‑2517784 | gcgcgcgcTTTCGAGCTAACGACAAAGTTCAGCCAAGCGGCATTCGGGCGAGCGCCCGGAGCGGTAAt < 1:147905/68‑1 (MQ=255) cgcgcgcTTTCGAGCTAACGACAAAGTTCAGCCAAGCGGCATTCGGGCGAGCGCCCGGAGCGGTGATg < 1:607088/68‑1 (MQ=255) cgcgcgcTTTCGAGCTAACGACAAAGTTCAGCCAAGCGGCATTCGGGCGAGCGCCCGGAGCGGTAATg < 1:754106/68‑1 (MQ=255) gCTAACGACAAAGTTCAGCCAAGCGGCATTCGGGCGAGCGCCCGGAGCGGTAAt < 1:869278/54‑1 (MQ=255) gCTAACGACAAAGTTCAGCCAAGCGGCATTCGGGCGAGCGCCCGGAGCGGTAATGATTTCAACGGTTTg < 1:129476/69‑1 (MQ=255) tAACGACAAAGTTCAGCCAAGCGGCATTCGGGCGAGCGCCCGGAGCGGTAATGATTTCAACGGTTTGAc < 1:1028307/69‑1 (MQ=255) cAGCCAAGCGGCATTCGGGCGAGCGCCCGGAGCGGTAATGATTTCAACGGTTTGACCGCTGGTAAGCgg > 1:1994462/1‑69 (MQ=255) cAGCCAAGCGGCATTCGGGCGAGCGCCCGGAGCGGTAATGATTTCAACGGTTTGACCGCTGGTAAGCgg > 1:76995/1‑69 (MQ=255) cAGCCAAGCGGCATTCGGGCGAGCGCCCGGAGCGGTAATGATTTCAACGGTTTGACCGCTGGTAAGCgg > 1:3330162/1‑69 (MQ=255) gCCACGCGGCATTCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCTGc < 1:2089309/68‑1 (MQ=255) gCCACGCGGCATTCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGc < 1:206809/68‑1 (MQ=255) gCCACGCGGCATTCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGc < 1:2063352/68‑1 (MQ=255) gCCACGCGGCATTCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGc < 1:2316279/68‑1 (MQ=255) gCCACGCGGCATTCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGc < 1:2436211/68‑1 (MQ=255) gCCACGCGGCATTCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGc < 1:1501337/68‑1 (MQ=255) gCCACGCGGCATTCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGc < 1:609152/68‑1 (MQ=255) gCCACGCGGCATTCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGc < 1:95149/68‑1 (MQ=255) gCCACGCGGCATTCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGc < 1:1058932/68‑1 (MQ=255) tCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGCTGCGACAGCgg > 1:2584629/1‑67 (MQ=255) tCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGCTGCGACAGCgg > 1:596683/1‑67 (MQ=255) tCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGCTGCGACAGCgg > 1:2741410/1‑67 (MQ=255) tCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGCTGCGACAGCgg > 1:3393175/1‑67 (MQ=255) tCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGCTGCGACAGCgg > 1:3419762/1‑67 (MQ=255) tCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGCTGCGACAGCgg > 1:2233868/1‑67 (MQ=255) tCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGCTGCGACAGCgg > 1:967569/1‑67 (MQ=255) tCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGCTGCGACAGCgg > 1:2444661/1‑67 (MQ=255) tCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGCTGCGACAGCgg > 1:1196785/1‑67 (MQ=255) tCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGCTGCGACAGCgg > 1:1333099/1‑67 (MQ=255) tCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGCTGCGACAGCgg > 1:1411940/1‑67 (MQ=255) tCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGCTGCGACAGCgg > 1:157464/1‑67 (MQ=255) tCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGCTGCGACAGCgg > 1:1623864/1‑67 (MQ=255) tCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGCTGCGACAGCgg > 1:1735348/1‑67 (MQ=255) tCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGCTGCGACAGCgg > 1:1792374/1‑67 (MQ=255) tCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGCTGCGACAGCgg > 1:1931039/1‑67 (MQ=255) tCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGCTGCGACAGCgg > 1:2457177/1‑67 (MQ=255) tCGGGCGAGCACCCGGTGCGGTAATGATTTCAACGGTTTGACCGCTAGTAAGCGGCTGCGACAGCg > 1:863642/1‑66 (MQ=255) cGGGCGAGCGCCCGGAGCGGTAATGATTTCAACGGTTTGACCGCTGGTAAGCGGCTGc > 1:1434650/1‑58 (MQ=255) cGGGCGAGCGCCCGGAGCGGTAATGATTTCAACGGTTTGACCGCTGGTAAGCGGCTGc > 1:1430251/1‑58 (MQ=255) cGGGCGAGCGCCCGGAGCGGTAATGATTTCAACGGTTTGACCGCTGGTAAGCGGCTGc > 1:1033123/1‑58 (MQ=255) cGGGCGAGCGCCCGGAGCGGTAATGATTTCAACGGTTTGACCGCTGGTAAGCGGCTGCGACAGCGGGTa > 1:3093535/1‑69 (MQ=255) cGGGCGAGCGCCCGGAGCGGTAATGATTTCAACGGTTTGACCGCTGGTAAGCGGCTGCGACAGCGGGTa > 1:1316419/1‑69 (MQ=255) cGGGCGAGCGCCCGGAGCGGTAATGATTTCAACGGTTTAACCGCTGGTAAGCGGCTGc > 1:522708/1‑58 (MQ=255) | GCGCGCGCTTTCGAGCTAACGACAAAGTTCAGCCAAGCGGCATTCGGGCGAGCGCCCGGAGCGGTAATGATTTCAACGGTTTGACCGCTGGTAAGCGGCTGCGACAGCGGGTA > minE/2517672‑2517784 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |