| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,517,962 | A→G | 33.9% | S371S (AGT→AGC) | spoT ← | bifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,517,962 | 0 | A | G | 33.9% | 32.4 / 25.7 | 50 | S371S (AGT→AGC) | spoT | bifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Reads supporting (aligned to +/- strand): ref base A (21/12); new base G (4/13); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.57e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.64e-01 | |||||||||||
GTGTGAAAACGTAAATCTCATCCGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGGCGCTCTGTTGCAGCTCCAGCAGGCTTTGCATCCAGCGCTGGGCGCGGATTTGTGCGG > minE/2517898‑2518026 | gtgtGAAAACGTAAATCTCATCCGGGTAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAGCTAc < 1:2479134/69‑1 (MQ=255)gtgtGAAAACGTAAATCTCATCCGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAGCTAc < 1:1876568/69‑1 (MQ=255)gtgtGAAAACGTAAATCTCATCCGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAGCTAc < 1:2872218/69‑1 (MQ=255)gtgtGAAAACGTAAATCTCATCCGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAGCTAc < 1:3157037/69‑1 (MQ=255)gtgtGAAAACGTAAATCTCATCCGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAGCTAc < 1:3228674/69‑1 (MQ=255)gtgtGAAAACGTAAATCTCATCCGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAGCTAc < 1:2380122/69‑1 (MQ=255)gtgtGAAAACGTAAATCTCATCCGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAGCTAc < 1:98691/69‑1 (MQ=255)gtgtGAAAACGTAAATCTCATCCGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAGCTAc < 1:1562976/69‑1 (MQ=255)gtgtGAAAACGTAAATCTCATCCGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAGCTAc < 1:750849/69‑1 (MQ=255)gtgtGAAAACGTAAATCTCATCCGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAGCTAc < 1:1314561/69‑1 (MQ=255)gtgtGAAAACGTAAATCTCATCCGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAGCTAc < 1:738342/69‑1 (MQ=255)gtgtGAAAACGTAAATCTCATCCGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAGCTAc < 1:1198218/69‑1 (MQ=255)gtgtGAAAACGTAAATCTCATCCGGGAAGAGATCGGATTTAACGATCTCGATAAATTCAAACGAGCTAc < 1:2927349/69‑1 (MQ=255) gtgAAAACGTAAATCTCATCCGGGAAGAGATCTGATTTAACGCTCTCGATAAATTCAAACGAACTACCg > 1:3200775/1‑69 (MQ=255) aaaaCGTAAATCTCATCCGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGgcg > 1:3335838/1‑69 (MQ=255) aaaaCGTAAATCTCATCCGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGgcg > 1:532846/1‑69 (MQ=255) aaaaCGTAAATCTCATCCGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGgcg > 1:350685/1‑69 (MQ=255) aaaaCGTAAATCTCATCCGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGgcg > 1:1740959/1‑69 (MQ=255) aaaaCGTAAATCTCATCCGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGgcg > 1:493615/1‑69 (MQ=255) aaCGTAGATCTCATCCGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGGCGct > 1:3354066/1‑69 (MQ=255) aaCGTAAATCTCATCCGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGGCGct > 1:766604/1‑69 (MQ=255) cGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGGCGCTCTGTTGCAGCTcc > 1:1846833/1‑67 (MQ=255) cGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGGCGCTCTGTTGCAGCTcc > 1:1705868/1‑67 (MQ=255) gAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAACTa > 1:1060187/1‑43 (MQ=255) gAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGGCGCTCTGTTGCAGCTCcagcag > 1:954963/1‑69 (MQ=255) gAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGGCGCTCTGTTGCAGCTCcagcag > 1:2962738/1‑69 (MQ=255) gAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGGCGCTCTGTTGCAGCTCcagcag > 1:285223/1‑69 (MQ=255) agagATCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGGCGCTCTGTTGCAGCTCCAGCAGGc < 1:476491/69‑1 (MQ=255) agagATCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGGCGCTCTGTTGCAGCTCCAGCAGGc < 1:2647908/69‑1 (MQ=255) agagATCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGGCGCTCTGTTGCAGCTCCAGCAGGc < 1:1026750/69‑1 (MQ=255) agagATCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGGCGCTCTGTTGCAGCTCCAGCAGGc < 1:2529537/69‑1 (MQ=255) agagATCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGGCGCTCTGTTGCAGCTCCAGCAGGc < 1:2340461/69‑1 (MQ=255) agagATCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGGCGCTCTGTTGCAGCTCCAGCAGGc < 1:2797199/69‑1 (MQ=255) agagATCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGGCGCTCTGTTGCAGCTCCAGCAGGc < 1:1285393/69‑1 (MQ=255) aTCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGGCGCTCTGTTGCAGCTCCAGCAGGCTTTg > 1:3050321/1‑69 (MQ=255) ctcGATAAATTCAAACGAACTACCGGCGCTCTGTTGCAGCTCCAGCAGGCTTTGCATCCAGCGCTGGgc > 1:3024700/1‑69 (MQ=255) ctcGATAAATTCAAACGAACTACCGGCGCTCTGTTGCAGCTCCAGCAGGCTTTGCATCCAGCGCTGGgc > 1:1097637/1‑69 (MQ=255) ctcGATAAATTCAAACGAACTACCGGCGCTCTGTTGCAGCTCCAGCAGGCTTTGCATCCAGCGCTGGgc > 1:2494321/1‑69 (MQ=255) ctcGATAAATTCAAACGAACTACCGGCGCTCTGTTGCAGCTCCAGCAGGCTTTGCATCCAGCGCTGGgc > 1:1396489/1‑69 (MQ=255) tAAATTCAAACGAACTACCGGCGCTCTGTTGCAGCTCCAGCAGGCTTTGCATCCAGCGCTGGGCGCGGa > 1:773934/1‑69 (MQ=255) tAAATTCAAACGAACTACCGGCGCTCTGTTGCAGCTCCAGCAGGCTTTGCATCCAGCGCTGGGCGCGGa > 1:2649435/1‑69 (MQ=255) aaaTTCAAACGAGCTACCGGCACTCTGTTGCAGCTCCAGCAGGCTTTGCATCCAGCGCTGGGcgcgta > 1:656632/1‑66 (MQ=255) aaaTTCAAACGAGCTACCGGCACTCTGTTGCAGCTCCAGCAGGCTTTGCATCCAGCGCTGGGCGCGTAt > 1:1506476/1‑69 (MQ=255) aaaTTCAAACGAGCTACCGGCACTCTGTTGCAGCTCCAGCAGGCTTTGCATCCAGCGCTGGGCGCGTAt > 1:2186710/1‑69 (MQ=255) aaaTTCAAACGAGCTACCGGCACTCTGTTGCAGCTCCAGCAGGCTTTGCATCCAGCGCTGGGCGCGTAt > 1:650403/1‑69 (MQ=255) aaaTTCAAACGAACTACCGGCGCTCTGTTGCAGCTCCAGCAGGCTTTGc < 1:103992/49‑1 (MQ=255) aCGAACTACCGGCGCTCTGTTGCAGCTCCAGCAGGCTTTGCATCCAGCGCTGGGCGCGGATTTGTGCgg < 1:2784405/69‑1 (MQ=255) aCGAACTACCGGCGCTCTGTTGCAGCTCCAGCAGGCTTTGCATCCAGCGCTGGGCGCGGATTTGTGCgg < 1:2189017/69‑1 (MQ=255) aCGAACTACCGGCGCTCTGTTGCAGCTCCAGCAGGCTTTGCATCCAGCGCTGGGCGCGGATTTGTGCgg < 1:1958436/69‑1 (MQ=255) aCGAACTACCGGCGCTCTGTTGCAGCTCCAGCAGGCTTTGCATCCAGCGCTGGGCGCGGATTTGTGCgg < 1:1723108/69‑1 (MQ=255) | GTGTGAAAACGTAAATCTCATCCGGGAAGAGATCGGATTTAACGCTCTCGATAAATTCAAACGAACTACCGGCGCTCTGTTGCAGCTCCAGCAGGCTTTGCATCCAGCGCTGGGCGCGGATTTGTGCGG > minE/2517898‑2518026 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |