| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 214,101 | T→C | 23.5% | F154F (TTT→TTC) | yafS → | predicted S‑adenosyl‑L‑methionine‑dependent methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 214,101 | 0 | T | C | 23.5% | 67.1 / 15.7 | 55 | F154F (TTT→TTC) | yafS | predicted S‑adenosyl‑L‑methionine‑dependent methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (25/17); new base C (3/10); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.86e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01 | |||||||||||
TTTATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTTTGAAGTGCTACACGCCAGCCGTTTCCA > minE/214036‑214169 | tttATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTAct > 1:1154519/1‑69 (MQ=255)tttATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTAct > 1:1191634/1‑69 (MQ=255)tttATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTAct > 1:101516/1‑69 (MQ=255) aTTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGc < 1:3335259/68‑1 (MQ=255) aTTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGc < 1:768947/68‑1 (MQ=255) aTTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGc < 1:1391254/68‑1 (MQ=255) aTTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGc < 1:1403562/68‑1 (MQ=255) aTTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGc < 1:2668743/68‑1 (MQ=255) tACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGc < 1:2505278/69‑1 (MQ=255) tACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGc < 1:2544681/69‑1 (MQ=255) aCGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAGCCGGATGTTCACTCTGATGCGGCa < 1:2116587/69‑1 (MQ=255) aCGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAGCCGGATGTTCACTCTGATGCGGCa < 1:923972/69‑1 (MQ=255) aCGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAGCCGGATGTTCACTCTGATGCGGCa < 1:2511165/69‑1 (MQ=255) aCGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAGCCGGATGTTCACTCTGATGCGGCa < 1:2718424/69‑1 (MQ=255) aCGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAGCCGGATGTTCACTCTGATGCGGCa < 1:2813443/69‑1 (MQ=255) aCGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAGCCGGATGTTCACTCTGATGCGGCa < 1:2817348/69‑1 (MQ=255) aCGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAGCCGGATGTTCACTCTGATGCGGCa < 1:3302819/69‑1 (MQ=255) aCGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAGCCGGATGTTCACTCTGATGCGGCa < 1:843974/69‑1 (MQ=255) aCGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAGCCGGATGTTCACTCTGATGCGGCa < 1:1743026/69‑1 (MQ=255) aCGCAAACTTGTGCCGGTATTGCGCAAAACATCGCCCTATAACAGCCGGATGTTCACTCTGATGCGGCa < 1:1613839/69‑1 (MQ=255) aaaCTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGc < 1:2954137/61‑1 (MQ=255) aaCTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTgg < 1:1531834/69‑1 (MQ=255) tATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCtt < 1:1096206/69‑1 (MQ=255) tATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCCCTCtt < 1:867913/69‑1 (MQ=255) ttGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTttg > 1:330919/1‑69 (MQ=255) gcgcAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTg < 1:3252395/52‑1 (MQ=255) gcgcAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTg < 1:3307413/52‑1 (MQ=255) gcgcAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTg < 1:3066190/52‑1 (MQ=255) gcgcAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTg < 1:71862/52‑1 (MQ=255) gcgcAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTg < 1:1219454/52‑1 (MQ=255) gcgcAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTg < 1:2979406/52‑1 (MQ=255) aCCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAAttt > 1:1952292/1‑68 (MQ=255) aCCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAAttt > 1:302534/1‑68 (MQ=255) aCCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAAttt > 1:498166/1‑68 (MQ=255) aCCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAAttt > 1:715271/1‑68 (MQ=255) aCAGCCGGATGTTTACTCTGATGCGGCAGTTGGACTGGCTCTCTTTGTTGAATTTTg > 1:2149330/1‑57 (MQ=255) aCAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGctct > 1:2084740/1‑42 (MQ=255) aCAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAAtttt > 1:1145772/1‑56 (MQ=255) aCAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTTTg > 1:902079/1‑57 (MQ=255) aCAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTTTg > 1:811345/1‑57 (MQ=255) aCAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTTTg > 1:2759913/1‑57 (MQ=255) aCAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTTTg > 1:216441/1‑57 (MQ=255) aCAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTTTGAAGTGCTacac > 1:3306883/1‑68 (MQ=255) aCAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTTTGAAGTGCTacac > 1:1066324/1‑68 (MQ=255) aCAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTTTGAAGTGCTacac > 1:1920484/1‑68 (MQ=255) aCAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTTTGAAGTGCTacac > 1:760627/1‑68 (MQ=255) aCAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTTTGAAGTGCTacac > 1:705834/1‑68 (MQ=255) aCAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTTTGAAGTGCTacac > 1:3336984/1‑68 (MQ=255) aCAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTTTGAAGTGCTacac > 1:3035433/1‑68 (MQ=255) aCAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTTTGAAGTGCTacac > 1:3191289/1‑68 (MQ=255) aCAGCCGGATGTTTACTCTGATGAGGCAGCTGGACTg > 1:3346807/1‑37 (MQ=255) aCAGCCGGATGTTTACACTGATGCGGCAGCTGGACTGGctc > 1:2375340/1‑41 (MQ=255) ggATGTTCACTCTGATGCGGCAGCTGGACTGGctct > 1:1849519/1‑36 (MQ=255) ggATGTTCACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTTTGAAGTGCTACACGCCAGcc > 1:231123/1‑69 (MQ=255) ggATGTTCACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTTTGAAGTGCTACACGCCAGcc > 1:2225953/1‑69 (MQ=255) tttACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTTTGAAGTGCTACACGCCAGCCGTTTc > 1:1873772/1‑69 (MQ=255) tttACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTTTGAAGTGCTACACGCCAGCCGTTTc > 1:3213127/1‑69 (MQ=255) tACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTTTGAAGTGCTACACGCCAGCCGTTTCCa > 1:3018192/1‑69 (MQ=255) tACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTTTGAAGTGCTACACGCCAGCCGTTTCCa > 1:1225284/1‑69 (MQ=255) | TTTATGGGATTACGCAAACTTGTGCCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTGGACTGGCTCTCTTTGTTGAATTTTGAAGTGCTACACGCCAGCCGTTTCCA > minE/214036‑214169 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |