| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,518,142 | G→A | 41.0% | I311I (ATC→ATT) | spoT ← | bifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,518,142 | 0 | G | A | 41.0% | 31.6 / 53.6 | 78 | I311I (ATC→ATT) | spoT | bifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Reads supporting (aligned to +/- strand): ref base G (25/21); new base A (15/17); total (40/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.40e-01 | |||||||||||
TCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTGGAATGGCGATA > minE/2518080‑2518193 | tcCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGatca > 1:2724280/1‑67 (MQ=255)tcCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGatca > 1:291809/1‑67 (MQ=255)tcCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGatca > 1:2886505/1‑67 (MQ=255)tcCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGatca > 1:153101/1‑67 (MQ=255)tcCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGatca > 1:3018411/1‑67 (MQ=255)tcCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGatca > 1:1455995/1‑67 (MQ=255)tcCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGatca > 1:1895664/1‑67 (MQ=255)tcCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGatca > 1:820750/1‑67 (MQ=255)tcCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGatca > 1:2012655/1‑67 (MQ=255)tcCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGatca > 1:2714629/1‑67 (MQ=255)tcCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGatca > 1:2351983/1‑67 (MQ=255)tcCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGatca > 1:2475577/1‑67 (MQ=255) tGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGgtgt > 1:866150/1‑67 (MQ=255) tGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGtgtg > 1:2942070/1‑68 (MQ=255) tGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGtgtg > 1:2832846/1‑68 (MQ=255) tGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGtgtg > 1:1757230/1‑68 (MQ=255) tGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGtgtg > 1:2476816/1‑68 (MQ=255) tGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGtgtg > 1:3114660/1‑68 (MQ=255) tGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGtgtg > 1:94013/1‑68 (MQ=255) tGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGtgtg > 1:946086/1‑68 (MQ=255) ccATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAg < 1:1481377/69‑1 (MQ=255) ccATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAg < 1:1089028/69‑1 (MQ=255) ccATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAg < 1:936324/69‑1 (MQ=255) atCTTCGGTACGGATCTGGACCTCAACCGGCACGCCGTGCGGGCCAATCATCGAGGTGTGCAAAGACt > 1:1032556/1‑68 (MQ=255) atCTTCGGTACGGATCTGGACCTCAACCGGCACGCCGTGCGGGCCAATCATCGAGGTGTGCAAAGACt > 1:2959038/1‑68 (MQ=255) atCTTCGGTACGGATCTGGACCTCAACCGGCACGCCGTGCGGGCCAATCATCGAGGTGTGCAAAGACt > 1:321963/1‑68 (MQ=255) atCTTCGGTACGGATCTGGACCTCAACCGGCACGCCGTGCGGGCCAATCATCGAGGTGTGCAAAGACt > 1:2788601/1‑68 (MQ=255) atCTTCGGTACGGATCTGGACCTCAACCGGCACGCCGTGCGGGCCAATCATCGAGGTGTGCAAAGACt > 1:2778625/1‑68 (MQ=255) atCTTCGGTACGGATCTGGACCTCAACCGGCACGCCGTGCGGGCCAATCATCGAGGTGTGCAAAGACt > 1:329637/1‑68 (MQ=255) atCTTCGGTACGGATCTGGACCTCAACCGGCACGCCGTGCGGGCCAATCATCGAGGTGTGCAAAGACt > 1:923661/1‑68 (MQ=255) atCTTCGGTACGGATCTGGACCTCAACCGGCACGCCGTGCGGGCCAATCATCGAGGTGTGCAAAGACt > 1:254291/1‑68 (MQ=255) atCTTCGGTACGGATCTGGACCTCAACCGGCACGCCGTGCGGGCCAATCATCGAGGTGTGCAAAGACt > 1:248084/1‑68 (MQ=255) atCTTCGGTACGGATCTGGACCTCAACCGGCACGCCGTGCGGGCCAATCATCGAGGTGTGCAAAGACt > 1:1067210/1‑68 (MQ=255) atCTTCGGTACGGATCTGGACCTCAACCGGCACGCCGTGCGGGCCAATCATCGAGGTGTGCAAAGACt > 1:1318826/1‑68 (MQ=255) atCTTCGGTACGGATCTGGACCTCAACCGGCACGCCGTGCGGGCCAATCATCGAGGTGTGCAAAGACt > 1:1331348/1‑68 (MQ=255) atCTTCGGTACGGATCTGGACCTCAACCGGCACGCCGTGCGGGCCAATCATCGAGGTGTGCAAAGACt > 1:1608496/1‑68 (MQ=255) atCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTg < 1:1590431/69‑1 (MQ=255) atCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTg < 1:1010292/69‑1 (MQ=255) atCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTg < 1:536075/69‑1 (MQ=255) atCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTg < 1:529272/69‑1 (MQ=255) atCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTg < 1:1492805/69‑1 (MQ=255) atCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTg < 1:1742815/69‑1 (MQ=255) atCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTg < 1:2694475/69‑1 (MQ=255) atCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTg < 1:2627879/69‑1 (MQ=255) atCTTCGGTACGGATCTGGAACTCAACCGGCACGCCGTGCGGGCCAATCATCGAGGTGTGCAAAGACt > 1:2909962/1‑68 (MQ=255) atCTTCGGTACGGAACTGGACCTCAACCGGCACGCCGTGCGGGCCAATCATCGAGGTGTGCAAAGACt > 1:2230039/1‑68 (MQ=255) tCTTCGGTACGGATCTGGACCTCAACCGGCCCACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACt > 1:1113423/1‑67 (MQ=255) tCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACt > 1:3381079/1‑67 (MQ=255) tCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACt > 1:3219826/1‑67 (MQ=255) ttCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTGATa > 1:2664769/1‑69 (MQ=255) ttCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTGATa > 1:2460407/1‑69 (MQ=255) ccTCAACCGGCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTgg < 1:1065618/67‑1 (MQ=255) ggCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTGGAATGGCGata < 1:1968130/69‑1 (MQ=255) ggCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTGGAATGGCGata < 1:428163/69‑1 (MQ=255) ggCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTGGAATGGCGata < 1:108964/69‑1 (MQ=255) ggCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTGGAATGGCGata < 1:750850/69‑1 (MQ=255) ggCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTGGAATGGCGata < 1:2607002/69‑1 (MQ=255) ggCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTGGAATGGCGata < 1:1256765/69‑1 (MQ=255) ggCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTGGAATGGCGata < 1:3147930/69‑1 (MQ=255) ggCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTGGAATGGCGata < 1:2574119/69‑1 (MQ=255) gCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTGGAATGGCGata < 1:2520769/68‑1 (MQ=255) gCGGGCCAATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTgg < 1:113693/49‑1 (MQ=255) gCGGGCCAATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTgg < 1:888910/49‑1 (MQ=255) gCGGGCCAATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTgg < 1:2244140/49‑1 (MQ=255) gCGGGCCAATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTgg < 1:939550/49‑1 (MQ=255) gCGGGCCAATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTgg < 1:641064/49‑1 (MQ=255) gCGGGCCAATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTgg < 1:2694114/49‑1 (MQ=255) gCGGGCCAATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTgg < 1:2434844/49‑1 (MQ=255) gCGGGCCAATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTgg < 1:428945/49‑1 (MQ=255) gCGGGCCAATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTgg < 1:3329744/49‑1 (MQ=255) gCGGGCCAATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTgg < 1:2606735/49‑1 (MQ=255) gCGGGCCAATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTgg < 1:173843/49‑1 (MQ=255) gCGGGCCAATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTgg < 1:189584/49‑1 (MQ=255) gCGGGCCAATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTgg < 1:2813194/49‑1 (MQ=255) gCGGGCCAATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTgg < 1:1924933/49‑1 (MQ=255) gCGGGCCAATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTgg < 1:2043273/49‑1 (MQ=255) gCGGGCCAATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTgg < 1:2671073/49‑1 (MQ=255) cGGGCCAATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTgg < 1:1904345/48‑1 (MQ=255) | TCCGCCATCTGGTCCATATCTTCGGTACGGATCTGGACCTCAACCGGCACACCGTGCGGGCCGATCATCGAGGTGTGCAAAGACTGATAGCCGTTCGCTTTTGGAATGGCGATA > minE/2518080‑2518193 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |