| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,520,256 | C→T | 28.3% | intergenic (‑210/‑48) | gmk ← / → ligB | guanylate kinase/DNA ligase, NAD(+)‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,520,256 | 0 | C | T | 28.3% | 136.0 / 52.3 | 123 | intergenic (‑210/‑48) | gmk/ligB | guanylate kinase/DNA ligase, NAD(+)‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (52/36); new base T (19/16); total (71/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01 | |||||||||||
Only 100 of 133 total aligned reads displayed.
CATTCACTGCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAGTATGGATGGCGA > minE/2520188‑2520322 | cATTCACTGCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGgctttgc > 1:3973/1‑69 (MQ=255)cATTCACTGCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGgctttgc > 1:2570185/1‑69 (MQ=255)cATTCACTGCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGgctttgc > 1:1431968/1‑69 (MQ=255) gCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGGCACTCGGCTTTGCTTtcatca > 1:242356/1‑69 (MQ=255) gCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTtcatca > 1:2179996/1‑69 (MQ=255) gCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTtcatca > 1:2276665/1‑69 (MQ=255) gCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTtcatca > 1:1595863/1‑69 (MQ=255) gCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTtcatca > 1:320925/1‑69 (MQ=255) gCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTtcatca > 1:1398360/1‑69 (MQ=255) gCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTtcatca > 1:2115532/1‑69 (MQ=255) gCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTtcatc > 1:2897918/1‑68 (MQ=255) gCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGACACTCGGCTTTGCTTtcatca > 1:777268/1‑69 (MQ=255) aTAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCAc < 1:1710012/67‑1 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTtcat > 1:2489589/1‑61 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGtt > 1:520540/1‑67 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:1527747/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:359818/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:1862739/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:3429049/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:3078812/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:1653791/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:1978612/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:2043886/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:2300704/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:3311440/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:2195591/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:2196644/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:1131573/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:1120228/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:953313/1‑68 (MQ=255) aGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCT‑CCTGACTATc < 1:2356099/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTTCACCAAGTTGTATATCTGCCTGACTATc < 1:2265857/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTTCACCAAGTTGTATATCTGCCTGACTATc < 1:3133505/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTTCACCAAGTTGTATATCTGCCTGACTATc < 1:3334413/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTTCACCAAGTTGTATATCTGCCTGACTATc < 1:505554/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTTCACCAAGTTGTATATCTGCCTGACTATc < 1:564427/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTTCACCAAGTTGTATATCTGCCTGACTATc < 1:3024117/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTTCACCAAGTTGTATATCTGCCTGACTATc < 1:749990/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTTCACCAAGTTGTATATCTGCCTGACTATc < 1:1151890/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTTCACCAAGTTGTATATCTGCCTGACTATc < 1:2682813/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc < 1:2750470/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc < 1:2632489/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc < 1:1716731/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc < 1:2260744/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc < 1:1461358/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc < 1:1005770/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc < 1:885281/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc < 1:1181429/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc < 1:1147808/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTATGCCACTCGGCTTTGTCTTCACTAAGTTGTATATCTGCCTGACTATc < 1:2517443/69‑1 (MQ=255) cGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTTCACCAAGTTGTATATCTGCCTGACTATc < 1:2573914/68‑1 (MQ=255) cGTCCTCGCAGACTCCGTTCTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc < 1:2129788/68‑1 (MQ=255) gTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTAt > 1:2780462/1‑66 (MQ=255) gTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc > 1:1610448/1‑67 (MQ=255) gTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc > 1:3010100/1‑67 (MQ=255) gTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc > 1:1198553/1‑67 (MQ=255) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:1028378/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:2685626/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:2871028/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:664687/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:518128/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:1651840/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:254144/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:2131180/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:2133491/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:1798222/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:2251672/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:229206/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:159542/1‑39 (MQ=25) aGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGa < 1:3043318/68‑1 (MQ=255) aGACTACGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGa < 1:1297658/68‑1 (MQ=255) ccGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACa > 1:3333259/1‑58 (MQ=255) ccGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGtgat > 1:1242561/1‑69 (MQ=255) ccGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGtgat > 1:3338304/1‑69 (MQ=255) ccGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGtgat > 1:2388000/1‑69 (MQ=255) ccGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGtgat < 1:2953168/69‑1 (MQ=255) ccGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGtgat < 1:2449066/69‑1 (MQ=255) ccGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGtgat > 1:2782360/1‑69 (MQ=255) ccGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGtgat > 1:1536323/1‑69 (MQ=255) ccGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACAATCTACAGCGGAGGtga > 1:1911333/1‑68 (MQ=255) cTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAGTatg < 1:471484/69‑1 (MQ=255) cTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAGTatg < 1:3167339/69‑1 (MQ=255) cTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAGTatg < 1:1545297/69‑1 (MQ=255) tCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:929145/64‑1 (MQ=255) cGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:627564/63‑1 (MQ=255) cGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:655360/63‑1 (MQ=255) cGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:1321805/63‑1 (MQ=255) cGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:1651008/63‑1 (MQ=255) cGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:816225/63‑1 (MQ=255) cGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:2377972/63‑1 (MQ=255) cGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:2523433/63‑1 (MQ=255) cGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:2582267/63‑1 (MQ=255) cGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:2631073/63‑1 (MQ=255) ggCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:2062669/62‑1 (MQ=255) ggCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:449696/62‑1 (MQ=255) tttgctttCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAGTATGGATGGc < 1:2432538/69‑1 (MQ=255) ctttCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAGTATGGATGGCGa < 1:2672879/67‑1 (MQ=255) ctttCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAGTATGGATGGCGa < 1:305396/67‑1 (MQ=255) ctttCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAGTATGGATGGCGa < 1:1207428/67‑1 (MQ=255) ctttCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAGTATGGATGGCGa < 1:1074673/67‑1 (MQ=255) | CATTCACTGCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAGTATGGATGGCGA > minE/2520188‑2520322 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |