| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,520,257 | T→C | 28.4% | intergenic (‑211/‑47) | gmk ← / → ligB | guanylate kinase/DNA ligase, NAD(+)‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,520,257 | 0 | T | C | 28.4% | 129.8 / 54.8 | 123 | intergenic (‑211/‑47) | gmk/ligB | guanylate kinase/DNA ligase, NAD(+)‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (52/36); new base C (19/16); total (71/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01 | |||||||||||
Only 100 of 130 total aligned reads displayed.
GCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAGTATGGATGGCGA > minE/2520196‑2520322 | gCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTtcatca > 1:2115532/1‑69 (MQ=255)gCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTtcatca > 1:2179996/1‑69 (MQ=255)gCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTtcatca > 1:1851391/1‑69 (MQ=255)gCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTtcatca > 1:320925/1‑69 (MQ=255)gCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTtcatc > 1:2897918/1‑68 (MQ=255)gCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGACACTCGGCTTTGCTTtcatca > 1:777268/1‑69 (MQ=255) aTAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCAc < 1:1710012/67‑1 (MQ=255) aTAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCAc < 1:2271740/67‑1 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCTCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:2528382/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTtcat > 1:2489589/1‑61 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGtt > 1:520540/1‑67 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:2317980/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:3311440/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:2043886/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:953313/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:2195591/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:1862739/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:1120228/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:2196644/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:1131573/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:1699546/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:3429049/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:507768/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:2579268/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:1527747/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:1498481/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:2300704/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:139815/1‑68 (MQ=255) aaGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTg > 1:3078812/1‑68 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTTCACCAAGTTGTATATCTGCCTGACTATc < 1:2265857/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTTCACCAAGTTGTATATCTGCCTGACTATc < 1:3024117/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTTCACCAAGTTGTATATCTGCCTGACTATc < 1:3133505/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTTCACCAAGTTGTATATCTGCCTGACTATc < 1:2682813/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTTCACCAAGTTGTATATCTGCCTGACTATc < 1:2653723/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTTCACCAAGTTGTATATCTGCCTGACTATc < 1:1151890/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTTCACCAAGTTGTATATCTGCCTGACTATc < 1:3334413/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTTCACCAAGTTGTATATCTGCCTGACTATc < 1:1997530/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTTCACCAAGTTGTATATCTGCCTGACTATc < 1:1634705/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTTCACCAAGTTGTATATCTGCCTGACTATc < 1:749990/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc < 1:1005770/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc < 1:1716731/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc < 1:2260744/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc < 1:1147808/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc < 1:1181429/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc < 1:885281/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc < 1:1461358/69‑1 (MQ=255) gCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc < 1:2750470/69‑1 (MQ=255) cGTCCTCGCAGACTCCGTTCTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc < 1:2129788/68‑1 (MQ=255) gTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTAt > 1:2780462/1‑66 (MQ=255) gTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc > 1:2415534/1‑67 (MQ=255) gTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc > 1:3010100/1‑67 (MQ=255) gTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc > 1:1198553/1‑67 (MQ=255) gTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc > 1:1610448/1‑67 (MQ=255) gTCCTAGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATc > 1:155351/1‑67 (MQ=255) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:2685626/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:1028378/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:3051108/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:2871028/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:664687/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:518128/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:3414701/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:3317926/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:229206/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:159542/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:1651840/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:2131180/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:2133491/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:2251672/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:2450241/1‑39 (MQ=25) cGCAGACTCCGTTTTTGCCACTCGGCTTTGTCTtcacca > 1:254144/1‑39 (MQ=25) aGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTATAGCGGa < 1:438594/68‑1 (MQ=255) aGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGa < 1:3043318/68‑1 (MQ=255) aGACTACGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGa < 1:1297658/68‑1 (MQ=255) ccGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGtgat < 1:2953168/69‑1 (MQ=255) ccGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGtgat > 1:3338304/1‑69 (MQ=255) ccGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGtgat > 1:2782360/1‑69 (MQ=255) ccGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGtgat > 1:1242561/1‑69 (MQ=255) ccGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGtgat > 1:2388000/1‑69 (MQ=255) ccGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGtgat < 1:2449066/69‑1 (MQ=255) ccGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACAATCTACAGCGGAGGtga > 1:1911333/1‑68 (MQ=255) ccACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:566749/68‑1 (MQ=255) cTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAGTatg < 1:471484/69‑1 (MQ=255) cTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAGTatg < 1:1545297/69‑1 (MQ=255) tCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:929145/64‑1 (MQ=255) cGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:655360/63‑1 (MQ=255) cGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:2631073/63‑1 (MQ=255) cGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:1321805/63‑1 (MQ=255) cGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:627564/63‑1 (MQ=255) cGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:816225/63‑1 (MQ=255) cGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:1651008/63‑1 (MQ=255) cGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:2377972/63‑1 (MQ=255) cGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:2523433/63‑1 (MQ=255) cGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:2582267/63‑1 (MQ=255) ggCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:449696/62‑1 (MQ=255) ggCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAg < 1:2062669/62‑1 (MQ=255) tttgctttCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAGTATGGATGGc < 1:2432538/69‑1 (MQ=255) ctttCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAGTATGGATGGCGa < 1:3146681/67‑1 (MQ=255) ctttCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAGTATGGATGGCGa < 1:1207428/67‑1 (MQ=255) ctttCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAGTATGGATGGCGa < 1:2672879/67‑1 (MQ=255) ctttCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAGTATGGATGGCGa < 1:1074673/67‑1 (MQ=255) | GCAATAAAGACGATTTTCAGGAAGCGTCCTCGCAGACTCCGTTTTTGCCACTCGGCTTTGCTTTCATCACGTTGTATATCTGCCTGACTATCTACAGCGGAGGTGATGATGAAAGTATGGATGGCGA > minE/2520196‑2520322 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |