| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,522,085 | C→A | 66.1% | V172V (GTG→GTT) | yicG ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,522,085 | 0 | C | A | 66.1% | 46.7 / 46.8 | 62 | V172V (GTG→GTT) | yicG | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (8/13); new base A (13/28); total (21/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
ATCCCAGCTTAAGGCGTAGCGCCAGTAAGCGGGCAAAAAAGCCGAATACCAGGGTGGAGATAATTACCACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATGA > minE/2522018‑2522144 | aTCCCAGCTTAAGGCGTAGCGCCAGTAAGCGGGCAAAAAAGCCGAATACCAGGGTGGAGATAATTACca > 1:2413323/1‑69 (MQ=255) ccAGCTTAAGGCGTAGCGCCAGTAAGCGGGCAAAAAAGCCGAATACCAGGGTGGAGATAATTACCAcat > 1:2690500/1‑69 (MQ=255) gCTTAAGGCGTAGCGCCAGTAAGCGGGCAAAAAAGCCGAATACCAGGGTGGAGATAATTACCAcatcat < 1:1946880/69‑1 (MQ=255) gcCAGTAAGCGGGCAAAAAAGCCGAATACCATGGTGGAGATAATTACCACATCATGGTTAGAAACATAg < 1:788391/69‑1 (MQ=255) gcCAGTAAGCGGGCAAAAAAGCCGAATACCAGGGTGGAGATAATTACCACATCATGGTTAGAAACATAg < 1:3284235/69‑1 (MQ=255) gcCAGTAAGCGGGCAAAAAAGCCGAATACCAGGGTGGAGATAATTACCACATCATGGTTAGAAACATAg < 1:1712761/69‑1 (MQ=255) gcCAGTAAGCGGGCAAAAAAGCCGAATACCAGGGTGGAGATAATTACCACATCATGGTTAGAAACATAg < 1:3041161/69‑1 (MQ=255) gcCAGTAAGCGGGCAAAAAAGCCGAATACCAGGGTGGAGATAATTACCACATCATGGTTAGAAACATAg < 1:3395847/69‑1 (MQ=255) gcCAGTAAGCGGGCAAAAAAGCCGAATACCAGGGTGGAGATAATTACCACATCATGGTTAGAAACATAg < 1:948414/69‑1 (MQ=255) aaaaaGCCGAATACCAGGGTGGAGATAATTACCACATCATGGTTAGAAACATAGTGTTGCAGCGCGATg < 1:2447908/69‑1 (MQ=255) aaaaaGCCGAATACCAGGGTGGAGATAATTACCACATCATGGTTAGAAACATAGTGTTGCAGCGCGATg < 1:2273273/69‑1 (MQ=255) aaaGCCGAATACCAGGGTGGAGATAATTACCACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTa < 1:3255289/69‑1 (MQ=255) aaaGCCGAATACCAGGGTGGAGATAATTACCACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTa < 1:345868/69‑1 (MQ=255) ccAGGGTGGAGATAATTACCACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCAcggcgg > 1:2609524/1‑69 (MQ=255) ccAGGGTGGAGATAATTACCACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCAcggcgg > 1:655276/1‑69 (MQ=255) ccAGGGTGGAGATAATTACCACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCAcggcgg > 1:690332/1‑69 (MQ=255) ccAGGGTGGAGATAATTACCACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCAcggcgg > 1:969154/1‑69 (MQ=255) ccAGGGTGGAGATAATTACCACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCAcggcgg > 1:1514980/1‑69 (MQ=255) ccAGGGTGGAGATAATTACCACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCAcggcg > 1:2962705/1‑68 (MQ=255) gggTGGAGATAATCACAACATCATGGTTAGAAACATAgtg > 1:562355/1‑40 (MQ=255) gggTGGAGATAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAg > 1:2517729/1‑68 (MQ=255) gggTGGAGATAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAg > 1:128712/1‑68 (MQ=255) gggTGGAGATAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAg > 1:3081427/1‑68 (MQ=255) gggTGGAGATAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAg > 1:782134/1‑68 (MQ=255) gggTGGAGATAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAg > 1:1627534/1‑68 (MQ=255) gggTGGAGATAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAg > 1:2577424/1‑68 (MQ=255) gggTGGAGATAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAg > 1:2548605/1‑68 (MQ=255) gggTGGAGATAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAg > 1:181694/1‑68 (MQ=255) gggTGGAGATAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAg > 1:2291913/1‑68 (MQ=255) gggTGGAGATAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAg > 1:1098703/1‑68 (MQ=255) gggTGGAGATAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAg > 1:2005343/1‑68 (MQ=255) gggTGGAGATAATCACAACATCATGGTTAGAAAAATAGTGTTGCAGCGCGATGTACAGCACGGCGGAg > 1:921845/1‑68 (MQ=255) tGGAGATAATTACCACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCaa > 1:793883/1‑69 (MQ=255) gaTAATTACCACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:3122954/69‑1 (MQ=255) gaTAATTACCACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:828175/69‑1 (MQ=255) gaTAATCACAACATCATGTTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:2973813/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACTGCGGAGGCAAATga < 1:291795/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:1113338/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:625578/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:1491475/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:1188660/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:726216/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:3071960/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:81459/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:950542/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:1022964/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:284387/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:1863043/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:2102650/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:2203395/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:2253656/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:1837192/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:1750402/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:2453319/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:1012084/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:3326447/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:2975213/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:3037240/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:1532367/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:1903579/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:1517475/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATga < 1:3325572/69‑1 (MQ=255) gaTAATCACAACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAACga < 1:1466335/69‑1 (MQ=255) | ATCCCAGCTTAAGGCGTAGCGCCAGTAAGCGGGCAAAAAAGCCGAATACCAGGGTGGAGATAATTACCACATCATGGTTAGAAACATAGTGTTGCAGCGCGATGTACAGCACGGCGGAGGCAAATGA > minE/2522018‑2522144 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |