| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,524,243 | C→T | 37.6% | V186I (GTC→ATC) ‡ | yicC ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,524,243 | 0 | C | T | 37.6% | 40.2 / 41.5 | 69 | V186I (GTC→ATC) ‡ | yicC | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (25/18); new base T (15/11); total (40/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01 | |||||||||||
AGAACCAGTTCCTGCTCCAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGAGCGGACTTTGACCACTTCGGCGGT > minE/2524179‑2524309 | agAACCAACTCCTGCTCCAGACGGTTGTTTTCCAGTTTCACCTGATCATCTTCCAGCTTCGCTATCAGa < 1:1702118/69‑1 (MQ=255)agAACCAACTCCTGCTCCAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCTATCAGa < 1:2468501/69‑1 (MQ=255)agAACCAACTCCTGCTCCAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCTATCAGa < 1:2048576/69‑1 (MQ=255)agAACCAACTCCTGCTCCAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCTATCAGa < 1:1727736/69‑1 (MQ=255)agAACCAACTCCTGCTCCAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCTATCAGa < 1:1652037/69‑1 (MQ=255)agAACCAACTCCTGCTCCAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCTATCAGa < 1:3312082/69‑1 (MQ=255)agAACCAACTCCTGCTCCAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCTATCAGa < 1:3296818/69‑1 (MQ=255)agAACCAACTCCTGCTCCAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCTATCAGa < 1:3226574/69‑1 (MQ=255)agAACCAACTCCTGCTCCAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCTATCAGa < 1:1308992/69‑1 (MQ=255)agAACCAACTCCTGCTCCAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCTATCAGa < 1:1278813/69‑1 (MQ=255)agAACCAACTCCTGCTCCAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCTATCAGa < 1:2922437/69‑1 (MQ=255) aaCCAGTTCCTGCTCCAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACg < 1:658128/69‑1 (MQ=255) aCCAGTTCCTGCTCCAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACg > 1:28588/1‑68 (MQ=255) aCCAGTTCCTGCTCCAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACg > 1:1000886/1‑68 (MQ=255) tCCTGCTCCAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGc > 1:2481527/1‑69 (MQ=255) tCCTGCTCCAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGc > 1:2305162/1‑69 (MQ=255) tCCTGCTCCAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGc > 1:1675186/1‑69 (MQ=255) tCCTGCTCCAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGc > 1:2203225/1‑69 (MQ=255) gCTCCAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGc < 1:2600548/68‑1 (MQ=255) gCTCCAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGc < 1:1307316/68‑1 (MQ=255) ccAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCAt > 1:1981414/1‑68 (MQ=255) ccAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCAt > 1:3332848/1‑68 (MQ=255) ccAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCAt < 1:2190083/68‑1 (MQ=255) ccAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCAt < 1:117095/68‑1 (MQ=255) ccAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCAt > 1:831065/1‑68 (MQ=255) ccAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCAt < 1:892304/68‑1 (MQ=255) ccAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCAt > 1:1349681/1‑68 (MQ=255) ccAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCAt > 1:1597399/1‑68 (MQ=255) aCGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCATTGCAg < 1:2790703/69‑1 (MQ=255) cGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCATTGCAgg > 1:1177714/1‑69 (MQ=255) cGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCATTGCAgg > 1:828634/1‑69 (MQ=255) cGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCATTGCAgg > 1:2177041/1‑69 (MQ=255) cGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCATTGCAgg > 1:909287/1‑69 (MQ=255) cGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCATTGCAgg > 1:2214223/1‑69 (MQ=255) cGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCATTGCAgg > 1:1906846/1‑69 (MQ=255) cAGTTGCACCTGAGCATCTTCCAGCTTCGGGACCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCa < 1:1980609/68‑1 (MQ=255) cAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCa < 1:1582310/68‑1 (MQ=255) cAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCa < 1:139948/68‑1 (MQ=255) cAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCa < 1:1498886/68‑1 (MQ=255) cAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCa < 1:936937/68‑1 (MQ=255) cAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCa < 1:2229140/68‑1 (MQ=255) cAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCa < 1:1836165/68‑1 (MQ=255) cATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGAGCGGACt > 1:2743786/1‑68 (MQ=255) tCTTACAGCTTCGCGACCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGAGCGGACTTTg > 1:2636898/1‑69 (MQ=255) aGCTTCGCGACCAGACGCTCACGCTGCCATTGCTGGATTTCCGGCATATGGGAGCGGACTTTGACCACt > 1:1572323/1‑69 (MQ=255) aGCTTCGCGACCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGAGCGGACTTTGACCAc < 1:2075048/68‑1 (MQ=255) aGCTTCGCGACCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGAGCGGACTTTGACCACt > 1:947662/1‑69 (MQ=255) aGCTTCGCGACCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGAGCGGACTTTGACCACt > 1:3253873/1‑69 (MQ=255) aGCTTCGCGACCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGAGCGGACTTTGACCACt > 1:994004/1‑69 (MQ=255) aGCTTCGCGACCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGAGCGGACTTTGACCACt > 1:3078824/1‑69 (MQ=255) aGCTTCGCGACCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGAGCGGACTTTGACCACt > 1:2974587/1‑69 (MQ=255) tCGCTATCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGCGCGGACTTTGGCCACTTcgg > 1:1109900/1‑69 (MQ=255) tCGCTATCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGCGCGGACTTTGGCCACTTcgg > 1:1483549/1‑69 (MQ=255) tCGCTATCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGCGCGGACTTTGGCCACTTcgg > 1:1572159/1‑69 (MQ=255) tCGCTATCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGCGCGGACTTTGGCCACTTcgg > 1:1816065/1‑69 (MQ=255) tCGCTATCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGCGCGGACTTTGGCCACTTcgg > 1:1864897/1‑69 (MQ=255) tCGCTATCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGCGCGGACTTTGGCCACTTcgg > 1:2085810/1‑69 (MQ=255) tCGCTATCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGCGCGGACTTTGGCCACTTcgg > 1:798326/1‑69 (MQ=255) tCGCTATCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGCGCGGACTTTGGCCACTTcgg > 1:2116168/1‑69 (MQ=255) tCGCTATCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGCGCGGACTTTGGCCACTTcgg > 1:592282/1‑69 (MQ=255) tCGCTATCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGCGCGGACTTTGGCCACTTcgg > 1:563833/1‑69 (MQ=255) tCGCTATCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGCGCGGACTTTGGCCACTTcgg > 1:2305427/1‑69 (MQ=255) tCGCTATCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGCGCGGACTTTGGCCACTTcgg > 1:2353890/1‑69 (MQ=255) tCGCTATCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGCGCGGACTTTGGCCACTTcgg > 1:2546158/1‑69 (MQ=255) tCGCTATCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGCGCGGACTTTGGCCACTTcgg > 1:2578750/1‑69 (MQ=255) tCGCTATCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGCGCGGACTTTGGCCACTTcgg > 1:3242057/1‑69 (MQ=255) gACCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGAGCGGACTTTGACCACTTCGGCGGt < 1:171369/69‑1 (MQ=255) gACCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGAGCGGACTTTGACCACTTCGGCGGt < 1:2783846/69‑1 (MQ=255) gACCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGAGCGGACTTTGACCACTTCGGCGGt < 1:3001508/69‑1 (MQ=255) | AGAACCAGTTCCTGCTCCAGACGGTTGTTTTCCAGTTGCACCTGAGCATCTTCCAGCTTCGCGACCAGACGCTCACGCTGCCATTGCAGGATTTCCGGCATATGGGAGCGGACTTTGACCACTTCGGCGGT > minE/2524179‑2524309 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |