| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,524,337 | T→C | 65.0% | A154A (GCA→GCG) ‡ | yicC ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,524,337 | 0 | T | C | 65.0% | 53.2 / 42.5 | 77 | A154A (GCA→GCG) ‡ | yicC | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (10/17); new base C (39/11); total (49/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.30e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
TCCGGCATATGGGAGCGGACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGACAATAAAGTCGTCCAGCGTACCATCCAGCGC > minE/2524272‑2524402 | tCCGGCATATGGGAGCGGACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCt < 1:305035/69‑1 (MQ=255)tCCGGCATATGGGAGCGGACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCt < 1:1752022/69‑1 (MQ=255)tCCGGCATATGGGAGCGGACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCt < 1:2857079/69‑1 (MQ=255)tCCGGCATATGGGAGCGGACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCt < 1:1534973/69‑1 (MQ=255)tCCGGCATATGGGAGCGGACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCt < 1:3224229/69‑1 (MQ=255)tCCGGCATATGGGAGCGGACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCt < 1:698972/69‑1 (MQ=255)tCCGGCATATGGGAGCGGACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCt < 1:117772/69‑1 (MQ=255) gCATATGGGAGCGGACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCTTTc < 1:3300406/68‑1 (MQ=255) gCATATGGGAGCGGACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCTTTc < 1:1705407/68‑1 (MQ=255) gCATATGGGAGCGGACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCTTTc < 1:320898/68‑1 (MQ=255) gCATATGGGAGCGGACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCTTTc < 1:545779/68‑1 (MQ=255) gCATATGGGAGCGGACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCTTTc < 1:3296401/68‑1 (MQ=255) atatGGGAGCGGACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCTTTc < 1:1508550/66‑1 (MQ=255) tGGGAGCGGACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCTTTc < 1:3071553/63‑1 (MQ=255) tGGGAGCGGACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCTTTc < 1:2195960/63‑1 (MQ=255) tGGGAGCGGACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCTTTc < 1:3429982/63‑1 (MQ=255) tGGGAGCGGACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCTTTCAGTGc > 1:2998241/1‑68 (MQ=255) gACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCTTTCAGTGCCTGACCTTc > 1:1063074/1‑69 (MQ=255) gACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCTTTCAGTGCCTGACCTTc > 1:3026421/1‑69 (MQ=255) gACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCTTTCAGTGCCTGACCTTc > 1:3022316/1‑69 (MQ=255) gACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCTTTCAGTGCCTGACCTTc > 1:616880/1‑69 (MQ=255) gACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCTTTCAGTGCCTGACCTTc > 1:2620708/1‑69 (MQ=255) gcggTGACGCCTTCCAGGCGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGat > 1:409199/1‑67 (MQ=37) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTc > 1:1731276/1‑39 (MQ=21) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTCAGTGCCt > 1:974843/1‑46 (MQ=25) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCgagat > 1:1407185/1‑67 (MQ=37) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGat > 1:1196974/1‑67 (MQ=38) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGat > 1:1198515/1‑67 (MQ=38) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGat > 1:3388674/1‑67 (MQ=38) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGat > 1:1230509/1‑67 (MQ=38) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGat > 1:365710/1‑67 (MQ=38) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGat > 1:30026/1‑67 (MQ=38) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGat > 1:457027/1‑67 (MQ=38) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGat > 1:465625/1‑67 (MQ=38) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGat > 1:2729766/1‑67 (MQ=38) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGat > 1:1182247/1‑67 (MQ=38) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGat > 1:1671200/1‑67 (MQ=38) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGat > 1:2594041/1‑67 (MQ=38) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGat > 1:1824665/1‑67 (MQ=38) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGat > 1:1828322/1‑67 (MQ=38) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGat > 1:2494516/1‑67 (MQ=38) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGat > 1:747933/1‑67 (MQ=38) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGat > 1:1099485/1‑67 (MQ=38) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGat > 1:1029399/1‑67 (MQ=38) gcggTGACGCCTTCCAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGat > 1:2080838/1‑67 (MQ=38) gcggTGACGCCTTACAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTcgcgcg > 1:1753336/1‑64 (MQ=37) ggTGACGCCTTCCAGACGCTGCTCGATCAATGCTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGACAAt < 1:479814/69‑1 (MQ=255) ccAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCTGTTTCGCGCGCGa < 1:3394974/54‑1 (MQ=25) ccAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGa < 1:25668/54‑1 (MQ=37) ccAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGa < 1:3227068/54‑1 (MQ=37) ccAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGa < 1:1998366/54‑1 (MQ=37) ccAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGa < 1:1373025/54‑1 (MQ=37) ccAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGa < 1:1480897/54‑1 (MQ=37) ccAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGa < 1:1536681/54‑1 (MQ=37) ccAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGa < 1:2506483/54‑1 (MQ=37) ccAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGa < 1:581088/54‑1 (MQ=37) ccAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGa < 1:566037/54‑1 (MQ=37) ccAGACGCTGCTCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGa < 1:2645757/54‑1 (MQ=37) gctgctCGATCAATGCTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGACAATAAAGTCGTCCAGCGTAc > 1:3048271/1‑69 (MQ=255) gctgctCGATCAATGCTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGACAATAAAGTCGTCCAGCGTAc > 1:2293976/1‑69 (MQ=255) gctCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGATGATAAAgtcatc > 1:497846/1‑58 (MQ=25) gctCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGATGATAAAgtcatc > 1:1775540/1‑58 (MQ=25) gctCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGATGATAAAgtcatc > 1:764254/1‑58 (MQ=25) gctCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGATGATAAAgtcatc > 1:1950063/1‑58 (MQ=25) gctCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGATGATAAAgtcatc > 1:2551661/1‑58 (MQ=25) gctCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGATGATAAAgtcatc > 1:2663257/1‑58 (MQ=25) gctCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGATGATAAAgtcatc > 1:333490/1‑58 (MQ=25) gctCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGATGATAAAgtcatc > 1:2769106/1‑58 (MQ=25) gctCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGATGATAAAgtcatc > 1:1631072/1‑58 (MQ=25) gctCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGATGATAAAgtcatc > 1:1331110/1‑58 (MQ=25) gctCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGATGATAAAgtcatc > 1:1182736/1‑58 (MQ=25) gctCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGATGATAAAgtcatc > 1:1211649/1‑58 (MQ=25) gctCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGATGATAAAgtcatc > 1:3262541/1‑58 (MQ=25) gctCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGAGCGCGATGATAAAgtcat > 1:251763/1‑55 (MQ=21) ctCAATCAACACTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGATGATAAAgtcatc > 1:1937293/1‑57 (MQ=25) cAATGCTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGACAATAAAGTCGTCCAGCGTACCATCCAgcgc > 1:3177217/1‑69 (MQ=255) cAATGCTTTCAGTGCCTGACCTTCGGATTCGCGCGCGACAATAAAgtcgtc > 1:2844149/1‑51 (MQ=255) | TCCGGCATATGGGAGCGGACTTTGACCACTTCGGCGGTGACGCCTTCCAGACGCTGCTCGATCAATGCTTTCAGTGCCTGACCTTCGGTTTCGCGCGCGACAATAAAGTCGTCCAGCGTACCATCCAGCGC > minE/2524272‑2524402 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |