| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,525,308 | G→T | 65.1% | pseudogene (384/688 nt) | rph → | Mutation in protein compared to W3110: p.Val138Ala; p.Val172Ile; p.Ile216Thr; p.Glu224_Glyfs*16 ECK3633:JW3618:b3643; ribonuclease PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,525,308 | 0 | G | T | 65.1% | 25.5 / 33.0 | 43 | pseudogene (384/688 nt) | rph | Mutation in protein compared to W3110: p.Val138Ala; p.Val172Ile; p.Ile216Thr; p.Glu224_Glyfs*16 ECK3633:JW3618:b3643; ribonuclease PH |
| Reads supporting (aligned to +/- strand): ref base G (6/9); new base T (13/15); total (19/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01 | |||||||||||
GGGTGAGTTCACCATTACGCTGGACTGCGACGTGCTTCAGGCTGATGGTGGCACGCGTACCGCGTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAA > minE/2525245‑2525376 | gggTGAGTTCACCATTACGCTGGACTGCGACGTGCTTCAGGCTGATGGTGGCACGCGTACCGCGTCGAt < 1:3291702/69‑1 (MQ=255)gggTGAGTTCACCATTACGCTGGACTGCGACGTGCTTCAGGCTGATGGTGGCACGCGTACCGCGTCGAt < 1:2350418/69‑1 (MQ=255)gggTGAGTTCACCATTACGCTGGACTGCGACGTGCTTCAGGCTGATGGTGGCACGCGTACCGCGTCGAt < 1:3338159/69‑1 (MQ=255)gggTGAGTTCACCATTACGCTGGACTGCGACGTGCTTCAGGCTGATGGTGGCACGCGTACCGCGTCGAt < 1:423115/69‑1 (MQ=255) gAGTTCACCATTACGCTGGACTGCGACGTGCTTCAGGCTGATGGTGGCACGCGTACCGCGTCGATTACg < 1:119789/69‑1 (MQ=255) gAGTTCACCATTACGCTGGACTGCGACGTGCTTCAGGCTGATGGTGGCACGCGTACCGCGTCGATTACg < 1:2758238/69‑1 (MQ=255) gAGTTCACCATTACGCTGGACTGCGACGTGCTTCAGGCTGATGGTGGCACGCGTACCGCGTCGATTACg < 1:2623855/69‑1 (MQ=255) cACCATTACGCTGGACTTCGACGTGCTTCAGGCTGATGGTGGCACGCGTACCGCGTCGATTACGGGTGc > 1:3057205/1‑69 (MQ=255) aCGCTGGACTGCGACGTGCTTCAGGCTGATGGTGGCACGCGTACCGCGTCGATTACGGGTGCCTGc > 1:2340363/1‑66 (MQ=255) aCGCTGGACTGCGACGTGCTTCAGGCTGATGGTGGCACGCGTACCGCGTCGATTACGGGTGCCTGCGTg > 1:2248467/1‑69 (MQ=255) cTGGACTGCGACGTGCTTCAGGCTGATGGTGGCACGGGTACCGCGTCGATTACGGGTGCCTGCGTGgcg > 1:1390557/1‑69 (MQ=255) cTGGACTGCGACGTGCTTCAGGCTGATGGTGGCACGCGTACCGCGTCGATTACGGGTGCCTGCGTGgcg > 1:1040854/1‑69 (MQ=255) cTGGACTGCGACGTGCTTCAGGCTGATGGTGGCACGCGTACCGCGTCGATTACGGGTGCCTGCGTGgcg > 1:2603904/1‑69 (MQ=255) aCGTGCTTCAGGCTGATGGTGGCACGCGTACCGCGTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATg < 1:1790602/69‑1 (MQ=255) gCTTCAGGCTGATGGTGGCACGCGTACCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATgc < 1:1758193/66‑1 (MQ=255) gCTTCAGGCTGATGGTGGCACGCGTACCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATgc < 1:1683234/66‑1 (MQ=255) gCTTCAGGCTGATGGTGGCACGCGTACCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATgc < 1:3382018/66‑1 (MQ=255) gCTTCAGGCTGATGGTGGCACGCGTACCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATgc < 1:1947322/66‑1 (MQ=255) gCTTCAGGCTGATGGTGGCACGCGTACCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATgc < 1:550364/66‑1 (MQ=255) gCTTCAGGCTGATGGTGGCACGCGTACCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATgc < 1:649553/66‑1 (MQ=255) gCTTCAGGCTGATGGTGGCACGCGTACCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATgc < 1:701991/66‑1 (MQ=255) gCTTCAGGCTGATGGTGGCACGCGTACCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATgc < 1:2358425/66‑1 (MQ=255) gCTTCAGGCTGATGGTGGCACGCGTACCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATgc < 1:702411/66‑1 (MQ=255) gCTTCAGGCTGATGGTGGCACGCGTACCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATgc < 1:131572/66‑1 (MQ=255) gCTTCAGGCTGATGGTGGCACGCGTACCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATgc < 1:1299268/66‑1 (MQ=255) gCTTCAGGCTGATGGTGGCACGCGTACCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATgc < 1:2759776/66‑1 (MQ=255) gCTTCAGGCTGATGGTGGCACGCGTACCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATgc < 1:3099526/66‑1 (MQ=255) gCTTCAGGCTGATGGTGGCACGCGTACCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATgc < 1:3130183/66‑1 (MQ=255) gCTGATGGTGGCACGCGTACCGCGTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATGCGCTACAGa < 1:1472757/67‑1 (MQ=255) tGATGGTGCCACGCGTACCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATgc < 1:1871160/57‑1 (MQ=39) aCCGCTTCGATTACTGGTGCCTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAg > 1:1231746/1‑69 (MQ=255) aCCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAg > 1:816584/1‑69 (MQ=255) aCCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAg > 1:3120050/1‑69 (MQ=255) aCCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAg > 1:2830982/1‑69 (MQ=255) aCCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAg > 1:2800018/1‑69 (MQ=255) aCCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAg > 1:2149473/1‑69 (MQ=255) aCCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAg > 1:1898809/1‑69 (MQ=255) aCCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAg > 1:1567426/1‑69 (MQ=255) aCCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAg > 1:1548219/1‑69 (MQ=255) aCCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAg > 1:1473425/1‑69 (MQ=255) aCCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAg > 1:1471507/1‑69 (MQ=255) aCCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAg > 1:1410266/1‑69 (MQ=255) aCCGCTTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAg > 1:1112817/1‑69 (MQ=255) gTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGaa < 1:3104589/69‑1 (MQ=255) | GGGTGAGTTCACCATTACGCTGGACTGCGACGTGCTTCAGGCTGATGGTGGCACGCGTACCGCGTCGATTACGGGTGCCTGCGTGGCGCTGGCAGATGCGCTACAGAAGCTGGTGGAAAACGGCAAGCTGAA > minE/2525245‑2525376 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |