Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,526,697 A→G 48.2% L95L (CTT→CTC)  ttk ← division inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,526,6970AG48.2% 2.3 / 122.8 134L95L (CTT→CTC) ttkdivision inhibitor
Reads supporting (aligned to +/- strand):  ref base A (30/39);  new base G (39/25);  total (69/65)
Fisher's exact test for biased strand distribution p-value = 5.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 134 total aligned reads displayed.

TAGCGCATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGTTG  >  minE/2526640‑2526760
                                                         |                                                               
tAGCGCATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAac                                                        >  1:2523103/1‑67 (MQ=255)
 aGCGCATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAAcaca                                                     >  1:101693/1‑69 (MQ=255)
     cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                 <  1:519180/69‑1 (MQ=255)
     cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                 <  1:760272/69‑1 (MQ=255)
     cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                 <  1:682131/69‑1 (MQ=255)
     cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                 <  1:648468/69‑1 (MQ=255)
     cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                 <  1:586311/69‑1 (MQ=255)
     cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                 <  1:207567/69‑1 (MQ=255)
     cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                 <  1:931936/69‑1 (MQ=255)
      aTGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                 <  1:1134521/68‑1 (MQ=255)
                aGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAGCCGAGAAGCAGCAACACAATCAGACGCAGgcgc                                      >  1:434111/1‑69 (MQ=255)
                aGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGgcgc                                      >  1:2247486/1‑69 (MQ=255)
                aGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGgcgc                                      >  1:1309486/1‑69 (MQ=255)
                aGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGgcgc                                      >  1:855239/1‑69 (MQ=255)
                aGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGgcgc                                      >  1:3370091/1‑69 (MQ=255)
                aGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGgcgc                                      >  1:1002671/1‑69 (MQ=255)
                aGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGgcgc                                      >  1:3010063/1‑69 (MQ=255)
                aGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGgcgc                                      >  1:1594506/1‑69 (MQ=255)
                  gATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGgcgcgc                                    >  1:2121241/1‑69 (MQ=255)
                  gATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGgcgcgc                                    >  1:1545156/1‑69 (MQ=255)
                   aTGCGGGTCAGTCCAGTATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCt                                   <  1:1788891/69‑1 (MQ=255)
                   aTGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCt                                   <  1:1887954/69‑1 (MQ=255)
                   aTGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCt                                   <  1:3234647/69‑1 (MQ=255)
                    tGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCtg                                  >  1:3033646/1‑69 (MQ=255)
                    tGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCtg                                  >  1:2119466/1‑69 (MQ=255)
                    tGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCtg                                  >  1:1425535/1‑69 (MQ=255)
                    tGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCt                                   <  1:82414/68‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGTAGCAACAACACGATCAGACGCAGGCGCGCtg                                  <  1:2525077/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                  <  1:1020709/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                  <  1:3257916/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                  <  1:2621939/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                  <  1:1212477/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                  <  1:1242686/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                  <  1:3039034/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                  <  1:2505947/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                  <  1:2352168/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                  <  1:903121/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                  <  1:220238/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                  <  1:1288579/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                  <  1:1636006/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                  <  1:139383/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                  <  1:60167/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                  <  1:1693032/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                  <  1:780895/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                  <  1:619732/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGCCGCGCtg                                  <  1:2590174/69‑1 (MQ=255)
                      cGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCtgtg                                <  1:201639/69‑1 (MQ=255)
                      cGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCtgtg                                <  1:1547118/69‑1 (MQ=255)
                      cGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCtgtg                                <  1:2741498/69‑1 (MQ=255)
                      cGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCtgtg                                <  1:1810084/69‑1 (MQ=255)
                           cAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCGGGCGCGCTGTGGTGTc                           >  1:490995/1‑69 (MQ=255)
                           cAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTc                           >  1:1687341/1‑69 (MQ=255)
                                      tACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCt                 >  1:1787507/1‑68 (MQ=255)
                                      tACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCt                 >  1:2901690/1‑68 (MQ=255)
                                      tACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCt                 >  1:1075759/1‑68 (MQ=255)
                                      tACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCt                 >  1:1256927/1‑68 (MQ=255)
                                       aCGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCt                 >  1:2254619/1‑67 (MQ=255)
                                               cAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCa       <  1:2934584/69‑1 (MQ=255)
                                               cAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCa       <  1:2880863/69‑1 (MQ=255)
                                               cAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCa       <  1:1102718/69‑1 (MQ=255)
                                                aaaaCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCa       <  1:1756163/68‑1 (MQ=255)
                                                 aaaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:73301/1‑68 (MQ=255)
                                                 aaaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:1714848/1‑68 (MQ=255)
                                                 aaaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:2289169/1‑68 (MQ=255)
                                                 aaaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:2178688/1‑68 (MQ=255)
                                                 aaaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAg      >  1:2560530/1‑68 (MQ=255)
                                                 aaaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAg      >  1:446543/1‑68 (MQ=255)
                                                 aaaCCGAGGAGCAACAACACCATCAGACGCAGGCGCGCTGTCGTGTCCTtc                       >  1:2561245/1‑51 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCa                    >  1:3238949/1‑53 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATc        >  1:2352656/1‑65 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCa       >  1:933706/1‑66 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:3011724/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:1156462/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:1255489/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:1348613/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:1416167/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:1580080/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:1643366/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:1672636/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:1878157/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:1989637/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:2224235/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:244944/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:2520275/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:3013073/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:2645750/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:2660722/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:3139538/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga     >  1:2860024/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAg      >  1:657673/1‑67 (MQ=255)
                                                   aCCGAGAAGCAGCAACACAATCAGACGCAGGCTCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt   <  1:821380/69‑1 (MQ=255)
                                                   aCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt   <  1:109499/69‑1 (MQ=255)
                                                   aCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt   <  1:2399082/69‑1 (MQ=255)
                                                   aCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt   <  1:2995452/69‑1 (MQ=255)
                                                   aCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt   <  1:1483525/69‑1 (MQ=255)
                                                   aCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt   <  1:155282/69‑1 (MQ=255)
                                                   aCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt   <  1:7420/69‑1 (MQ=255)
                                                   aCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt   <  1:355946/69‑1 (MQ=255)
                                                    ccGAGAATCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGTTg  <  1:197582/69‑1 (MQ=255)
                                                    ccGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGTTg  <  1:877751/69‑1 (MQ=255)
                                                         |                                                               
TAGCGCATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGTTG  >  minE/2526640‑2526760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑