Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,526,702 G→A 48.5% L94L (CTG→TTG)  ttk ← division inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,526,7020GA48.5% 9.7 / 119.7 134L94L (CTG→TTG) ttkdivision inhibitor
Reads supporting (aligned to +/- strand):  ref base G (30/39);  new base A (39/26);  total (69/65)
Fisher's exact test for biased strand distribution p-value = 6.00e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

TAGCGCATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGTTGATGCGAGT  >  minE/2526640‑2526768
                                                              |                                                                  
 aGCGCATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAAcaca                                                             >  1:101693/1‑69 (MQ=255)
     cATGACCAGTGAGGATGCGGGTCAGGCCAGTATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                         <  1:2066635/69‑1 (MQ=255)
     cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                         <  1:931936/69‑1 (MQ=255)
     cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                         <  1:760272/69‑1 (MQ=255)
     cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                         <  1:682131/69‑1 (MQ=255)
     cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                         <  1:648468/69‑1 (MQ=255)
     cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                         <  1:586311/69‑1 (MQ=255)
     cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                         <  1:519180/69‑1 (MQ=255)
     cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                         <  1:1679308/69‑1 (MQ=255)
     cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                         <  1:1628671/69‑1 (MQ=255)
      aTGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                         <  1:2365839/68‑1 (MQ=255)
      aTGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                         <  1:1134521/68‑1 (MQ=255)
                aGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAGCCGAGAAGCAGCAACACAATCAGACGCAGgcgc                                              >  1:434111/1‑69 (MQ=255)
                aGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGgcgc                                              >  1:1002671/1‑69 (MQ=255)
                aGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGgcgc                                              >  1:1309486/1‑69 (MQ=255)
                aGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGgcgc                                              >  1:855239/1‑69 (MQ=255)
                  gATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGgcgcgc                                            >  1:2121241/1‑69 (MQ=255)
                  gATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGgcgcgc                                            >  1:1545156/1‑69 (MQ=255)
                   aTGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCt                                           <  1:1887954/69‑1 (MQ=255)
                    tGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCtg                                          >  1:2506529/1‑69 (MQ=255)
                    tGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCtg                                          >  1:1425535/1‑69 (MQ=255)
                    tGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCtg                                          >  1:2119466/1‑69 (MQ=255)
                    tGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCt                                           <  1:82414/68‑1 (MQ=255)
                    tGCGAGTCAGTCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:2132613/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGTAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:2525077/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:220238/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:2347305/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:1212477/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:2505947/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:3191509/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:2883850/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:3039034/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:1288579/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:3257916/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:60167/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:619732/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:780895/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:903121/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:1020709/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:1636006/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:1242686/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:1693032/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:139383/69‑1 (MQ=255)
                    tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGCCGCGCtg                                          <  1:2590174/69‑1 (MQ=255)
                     gCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:1960846/68‑1 (MQ=255)
                      cGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCtgtg                                        <  1:1810084/69‑1 (MQ=255)
                      cGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCtgtg                                        <  1:201639/69‑1 (MQ=255)
                      cGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCtgtg                                        >  1:2603777/1‑69 (MQ=255)
                           cAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCGGGCGCGCTGTGGTGTc                                   >  1:490995/1‑69 (MQ=255)
                           cAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTc                                   >  1:1687341/1‑69 (MQ=255)
                                     ttACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                         >  1:3002483/1‑37 (MQ=255)
                                      tACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCt                         >  1:1787507/1‑68 (MQ=255)
                                      tACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCt                         >  1:1075759/1‑68 (MQ=255)
                                      tACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCt                         >  1:1256927/1‑68 (MQ=255)
                                       aCGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATc                          >  1:2687040/1‑66 (MQ=255)
                                       aCGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCt                         >  1:2254619/1‑67 (MQ=255)
                                       aCGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCt                         >  1:3016163/1‑67 (MQ=255)
                                               cAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCa               <  1:2934584/69‑1 (MQ=255)
                                               cAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCa               <  1:1102718/69‑1 (MQ=255)
                                               cAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCa               <  1:2880863/69‑1 (MQ=255)
                                                aaaaCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCa               <  1:1756163/68‑1 (MQ=255)
                                                 aaaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga             >  1:3326367/1‑68 (MQ=255)
                                                 aaaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga             >  1:2289169/1‑68 (MQ=255)
                                                 aaaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga             >  1:73301/1‑68 (MQ=255)
                                                 aaaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga             >  1:1807499/1‑68 (MQ=255)
                                                 aaaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAg              >  1:446543/1‑68 (MQ=255)
                                                 aaaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAg              >  1:2560530/1‑68 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCa                            >  1:3238949/1‑53 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATc                >  1:2352656/1‑65 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCa               >  1:933706/1‑66 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga             >  1:1156462/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga             >  1:1580080/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga             >  1:1551865/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga             >  1:1255489/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga             >  1:3011724/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga             >  1:1416167/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga             >  1:1348613/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga             >  1:1643366/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga             >  1:3139538/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga             >  1:3013073/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga             >  1:2224235/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga             >  1:2860024/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga             >  1:2660722/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga             >  1:2228820/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga             >  1:2645750/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga             >  1:2538898/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga             >  1:2520275/1‑67 (MQ=255)
                                                  aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAg              >  1:657673/1‑67 (MQ=255)
                                                   aCCGAGAAGCAGCAACACAATCAGACGCAGGCTCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt           <  1:821380/69‑1 (MQ=255)
                                                   aCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt           <  1:355946/69‑1 (MQ=255)
                                                   aCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt           <  1:2399082/69‑1 (MQ=255)
                                                   aCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt           <  1:1483525/69‑1 (MQ=255)
                                                   aCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt           <  1:7420/69‑1 (MQ=255)
                                                   aCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt           <  1:109499/69‑1 (MQ=255)
                                                   aCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt           <  1:155282/69‑1 (MQ=255)
                                                   aCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt           <  1:2995452/69‑1 (MQ=255)
                                                    ccGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                          <  1:1734116/37‑1 (MQ=25)
                                                    ccGAGAATCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGTTg          <  1:197582/69‑1 (MQ=255)
                                                    ccGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGTTg          <  1:877751/69‑1 (MQ=255)
                                                            cagcaACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGTTGATGCGAGt  <  1:1859593/69‑1 (MQ=255)
                                                              |                                                                  
TAGCGCATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGTTGATGCGAGT  >  minE/2526640‑2526768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑