Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,526,709 A→G 48.5% I91I (ATT→ATC)  ttk ← division inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,526,7090AG48.5% ‑0.8 / 123.0 133I91I (ATT→ATC) ttkdivision inhibitor
Reads supporting (aligned to +/- strand):  ref base A (28/40);  new base G (38/26);  total (67/66)
Fisher's exact test for biased strand distribution p-value = 5.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 145 total aligned reads displayed.

AGCGCATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGTTGATGCGAGTAATCAGGCT  >  minE/2526641‑2526777
                                                                    |                                                                    
aGCGCATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAAcaca                                                                      >  1:101693/1‑69 (MQ=255)
    cATGACCAGTGAGGATGCGGGTCAGGCCAGTATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                                  <  1:2066635/69‑1 (MQ=255)
    cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                                  <  1:931936/69‑1 (MQ=255)
    cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                                  <  1:760272/69‑1 (MQ=255)
    cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                                  <  1:682131/69‑1 (MQ=255)
    cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                                  <  1:636414/69‑1 (MQ=255)
    cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                                  <  1:586311/69‑1 (MQ=255)
    cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                                  <  1:207567/69‑1 (MQ=255)
    cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                                  <  1:1679308/69‑1 (MQ=255)
    cATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                                  <  1:1628671/69‑1 (MQ=255)
     aTGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                                  <  1:2365839/68‑1 (MQ=255)
     aTGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                                  <  1:1134521/68‑1 (MQ=255)
               aGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGgcgc                                                       >  1:1309486/1‑69 (MQ=255)
               aGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGgcgc                                                       >  1:1002671/1‑69 (MQ=255)
               aGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGgcgc                                                       >  1:2247486/1‑69 (MQ=255)
               aGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGgcgc                                                       >  1:571835/1‑69 (MQ=255)
                 gATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGgcgcgc                                                     >  1:1545156/1‑69 (MQ=255)
                 gATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGgcgcgc                                                     >  1:2121241/1‑69 (MQ=255)
                  aTGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCt                                                    <  1:1887954/69‑1 (MQ=255)
                  aTGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCt                                                    <  1:3234647/69‑1 (MQ=255)
                   tGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCtg                                                   >  1:1425535/1‑69 (MQ=255)
                   tGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCtg                                                   >  1:2506529/1‑69 (MQ=255)
                   tGCGAGTCAGTCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                                   <  1:2132613/69‑1 (MQ=255)
                   tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGTAGCAACAACACGATCAGACGCAGGCGCGCtg                                                   <  1:2525077/69‑1 (MQ=255)
                   tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                                   <  1:1212477/69‑1 (MQ=255)
                   tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                                   <  1:438093/69‑1 (MQ=255)
                   tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                                   <  1:2621939/69‑1 (MQ=255)
                   tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                                   <  1:220238/69‑1 (MQ=255)
                   tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                                   <  1:3039034/69‑1 (MQ=255)
                   tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                                   <  1:1693032/69‑1 (MQ=255)
                   tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                                   <  1:139383/69‑1 (MQ=255)
                   tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                                   <  1:903121/69‑1 (MQ=255)
                   tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                                   <  1:1636006/69‑1 (MQ=255)
                   tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                                   <  1:1020709/69‑1 (MQ=255)
                   tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                                   <  1:619732/69‑1 (MQ=255)
                   tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                                   <  1:2505947/69‑1 (MQ=255)
                   tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                                   <  1:2347305/69‑1 (MQ=255)
                   tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                                   <  1:780895/69‑1 (MQ=255)
                   tGCGAGTCAGGCCAGGATTACGCTCACCAAAACCGAGGAGCAACAACACGATCAGACGCAGCCGCGCtg                                                   <  1:2590174/69‑1 (MQ=255)
                     cGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCtgtg                                                 <  1:1458888/69‑1 (MQ=255)
                     cGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCtgtg                                                 <  1:1810084/69‑1 (MQ=255)
                          cAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCGGGCGCGCTGTGGTGTc                                            >  1:490995/1‑69 (MQ=255)
                                    ttACGCTCACCAAAACCGAGAAGCAGCAACACAATCa                                                                  >  1:3002483/1‑37 (MQ=255)
                                     tACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCt                                  >  1:1787507/1‑68 (MQ=255)
                                     tACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCt                                  >  1:2901690/1‑68 (MQ=255)
                                     tACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCt                                  >  1:1256927/1‑68 (MQ=255)
                                     tACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCt                                  >  1:1075759/1‑68 (MQ=255)
                                      aCGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATc                                   >  1:2687040/1‑66 (MQ=255)
                                      aCGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCt                                  >  1:3016163/1‑67 (MQ=255)
                                              cAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCa                        <  1:2934584/69‑1 (MQ=255)
                                              cAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCa                        <  1:2880863/69‑1 (MQ=255)
                                               aaaaCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCa                        <  1:1756163/68‑1 (MQ=255)
                                                aaaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:2289169/1‑68 (MQ=255)
                                                aaaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:2178688/1‑68 (MQ=255)
                                                aaaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:1237076/1‑68 (MQ=255)
                                                aaaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:3326367/1‑68 (MQ=255)
                                                aaaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:1807499/1‑68 (MQ=255)
                                                aaaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:73301/1‑68 (MQ=255)
                                                aaaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAg                       >  1:446543/1‑68 (MQ=255)
                                                aaaCCGAGGAGCAACAACACCATCAGACGCAGGCGCGCTGTCGTGTCCTtc                                        >  1:2561245/1‑51 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCa                                     >  1:3238949/1‑53 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATc                         >  1:2352656/1‑65 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCa                        >  1:933706/1‑66 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:1259557/1‑67 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:1117812/1‑67 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:1580080/1‑67 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:1672636/1‑67 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:1255489/1‑67 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:2860024/1‑67 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:1878157/1‑67 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:1551865/1‑67 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:831727/1‑67 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:1989637/1‑67 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:1416167/1‑67 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:3139538/1‑67 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:2228820/1‑67 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:3013073/1‑67 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:3011724/1‑67 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:2538898/1‑67 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:2645750/1‑67 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAga                      >  1:1348613/1‑67 (MQ=255)
                                                 aaCCGAGGAGCAACAACACGATCAGACGCAGGCGCGCTGTGGTGTCCTTCTCATCTTTCAGAATCAg                       >  1:657673/1‑67 (MQ=255)
                                                  aCCGAGAAGCAGCAACACAATCAGACGCAGGCTCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt                    <  1:821380/69‑1 (MQ=255)
                                                  aCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt                    <  1:2399082/69‑1 (MQ=255)
                                                  aCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt                    <  1:355946/69‑1 (MQ=255)
                                                  aCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt                    <  1:7420/69‑1 (MQ=255)
                                                  aCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt                    <  1:2995452/69‑1 (MQ=255)
                                                  aCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt                    <  1:109499/69‑1 (MQ=255)
                                                  aCCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGtt                    <  1:155282/69‑1 (MQ=255)
                                                   ccGAGGAGCAACAACACGATCAGACGCAGGCGCGCtg                                                   <  1:1734116/37‑1 (MQ=25)
                                                   ccGAGAATCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGTTg                   <  1:197582/69‑1 (MQ=255)
                                                   ccGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGTTg                   <  1:877751/69‑1 (MQ=255)
                                                                    aaTCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGTTGATGCGAGTAATCAGGCt  <  1:1709694/69‑1 (MQ=255)
                                                                    aaTCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGTTGATGCGAGTAATCAGGCt  <  1:364284/69‑1 (MQ=255)
                                                                    aaTCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGTTGATGCGAGTAATCAGGCt  <  1:1493476/69‑1 (MQ=255)
                                                                    aaTCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGTTGATGCGAGTAATCAGGCt  <  1:3346965/69‑1 (MQ=255)
                                                                    aaTCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGTTGATGCGAGTAATCAGGCt  <  1:85310/69‑1 (MQ=255)
                                                                    aaTCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGTTGATGCGAGTAATCAGGCt  <  1:2190975/69‑1 (MQ=255)
                                                                    aaTCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGTTGATGCGAGTAATCAGGCt  <  1:2684577/69‑1 (MQ=255)
                                                                    aaTCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGTTGATGCGAGTAATCAGGCt  <  1:2691941/69‑1 (MQ=255)
                                                                    |                                                                    
AGCGCATGACCAGTGAGGATGCGGGTCAGGCCAGGATTACGCTCACCAAAACCGAGAAGCAGCAACACAATCAGACGCAGGCGCGCTGTGGTGTCTTTCTCATCTTTCAGAATCAGGTTGATGCGAGTAATCAGGCT  >  minE/2526641‑2526777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑