| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,530,970 | G→T | 48.9% | T216T (ACG→ACT) | mutM → | formamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,530,970 | 0 | G | T | 48.9% | 15.3 / 105.8 | 96 | T216T (ACG→ACT) | mutM | formamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase |
| Reads supporting (aligned to +/- strand): ref base G (23/26); new base T (15/32); total (38/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.86e-01 | |||||||||||
GTGTGAATTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGTTTACGG > minE/2530904‑2531035 | gtgtGAATTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGc > 1:2083713/1‑68 (MQ=255)gtgtGAATTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGc > 1:1344232/1‑68 (MQ=255)gtgtGAATTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGc > 1:1988767/1‑68 (MQ=255)gtgtGAATTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGc > 1:254001/1‑68 (MQ=255)gtgtGAATTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGc > 1:3059657/1‑68 (MQ=255)gtgtGAATTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGc > 1:658900/1‑68 (MQ=255)gtgtGAATTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGATCGATTGAGCAGGGTGGTACAACGc > 1:1583907/1‑68 (MQ=255) aaTTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAg < 1:3377444/69‑1 (MQ=255) aaTTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAg < 1:756111/69‑1 (MQ=255) aaaGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGa < 1:2423779/65‑1 (MQ=255) aaaGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGa < 1:231059/65‑1 (MQ=255) aaaGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGa < 1:2105587/65‑1 (MQ=255) aaaGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGa < 1:1887259/65‑1 (MQ=255) aaaGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGa < 1:1576936/65‑1 (MQ=255) gtTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGATTTTCt < 1:1617788/48‑1 (MQ=255) gtTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGATTTTCt < 1:1316944/48‑1 (MQ=255) cGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGa > 1:999095/1‑35 (MQ=255) cGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGa > 1:1832174/1‑35 (MQ=255) cGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGa > 1:1547002/1‑35 (MQ=255) cGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGa > 1:1311891/1‑35 (MQ=255) cGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGa > 1:1343879/1‑35 (MQ=255) cGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGa > 1:1272727/1‑35 (MQ=255) tCGATTGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCt < 1:3322442/69‑1 (MQ=255) ttGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAgg < 1:1244324/69‑1 (MQ=255) ttGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAgg < 1:2723068/69‑1 (MQ=255) ttGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAgg < 1:2719131/69‑1 (MQ=255) ttGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAgg < 1:2573358/69‑1 (MQ=255) ttGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAgg < 1:3106473/69‑1 (MQ=255) ttGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAgg < 1:2628568/69‑1 (MQ=255) ttGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAgg < 1:1711104/69‑1 (MQ=255) tGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCTGGCTATTTCGCTCAGGa < 1:2428402/69‑1 (MQ=255) tGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGa < 1:2908289/69‑1 (MQ=255) tGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGa < 1:2608540/69‑1 (MQ=255) tGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGa < 1:2678461/69‑1 (MQ=255) tGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGa < 1:2903853/69‑1 (MQ=255) aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATgg > 1:61467/1‑40 (MQ=255) aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATg > 1:757066/1‑39 (MQ=255) aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:2513195/1‑66 (MQ=255) aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:2600378/1‑66 (MQ=255) aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:3232435/1‑66 (MQ=255) aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:1080721/1‑66 (MQ=255) aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:2864991/1‑66 (MQ=255) aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:176845/1‑66 (MQ=255) aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:1496096/1‑66 (MQ=255) aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:1520892/1‑66 (MQ=255) aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:1678569/1‑66 (MQ=255) aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:640029/1‑66 (MQ=255) aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:1771942/1‑66 (MQ=255) aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:556863/1‑66 (MQ=255) aGGGTGGTACAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCGCAGGAAt > 1:1253285/1‑66 (MQ=255) tggtACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAgg > 1:2637180/1‑68 (MQ=255) gtACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCg > 1:1208607/1‑41 (MQ=255) gtACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGttt > 1:1128159/1‑69 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:2948372/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:289478/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:382763/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:956120/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:954002/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:849975/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:554481/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1100859/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1058302/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1028915/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:3099339/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:3053457/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:585819/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:27013/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1310792/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:15833/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1732763/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1738055/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1807408/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1253475/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1883352/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:189307/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1898386/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:2147867/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1224161/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1193331/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:2335497/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:2337897/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:2566433/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:277150/39‑1 (MQ=255) cAACTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:2632844/39‑1 (MQ=255) cAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGTATTGCAGGTTTACg < 1:1751000/69‑1 (MQ=255) cAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGTTTACg < 1:647989/69‑1 (MQ=255) cAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGTTTACg < 1:2675243/69‑1 (MQ=255) aaCTCTGAAAGATTTTCTGCAAAGTGATGGTAAACCgg < 1:1258430/38‑1 (MQ=255) aCGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGtt > 1:1557815/1‑63 (MQ=255) aCGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGtt > 1:957235/1‑63 (MQ=255) aCGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGtt > 1:94167/1‑63 (MQ=255) aCGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGtt > 1:902962/1‑63 (MQ=255) aCGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGtt > 1:1036469/1‑63 (MQ=255) aCGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGtt > 1:549626/1‑63 (MQ=255) aCGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGTTTAcag > 1:715113/1‑66 (MQ=255) aCGCTGAAAGATTATCTGCAAAGTGATGGTAAACCGGGCTATTTCGCt < 1:1155309/48‑1 (MQ=255) | GTGTGAATTGTTAGCTCGGGTGATTAAAGCGGTGTTGCTGCGTTCGATTGAGCAGGGTGGTACAACGCTGAAAGATTTTCTGCAAAGTGATGGTAAACCGGGCTATTTCGCTCAGGAATTGCAGGTTTACGG > minE/2530904‑2531035 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |