| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,531,297 | C→A | 17.8% | L118L (CTG→CTT) | coaD ← | pantetheine‑phosphate adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,531,297 | 0 | C | A | 17.8% | 127.0 / 14.2 | 61 | L118L (CTG→CTT) | coaD | pantetheine‑phosphate adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (40/10); new base A (7/4); total (47/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCCCTGATGGCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAGTGGCGATTCATATGCGCCAGCTGCATTTCATA > minE/2531230‑2531359 | cGCCCTGATGGCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATc > 1:1052998/1‑68 (MQ=255)cGCCCTGATGGCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATc > 1:199241/1‑68 (MQ=255)agCCCTGATGGCGCGACACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATc > 1:1201753/2‑68 (MQ=255) cccTGATGGCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATAAGa > 1:48283/1‑69 (MQ=255) cccTGATGGCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATAAGa > 1:2801780/1‑69 (MQ=255) cccTGATGGCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATAAGa > 1:694513/1‑69 (MQ=255) cccTGATGGCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATAAGa > 1:1729513/1‑69 (MQ=255) cccTGATGGCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATAAGa > 1:1363619/1‑69 (MQ=255) cccTGATGGCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATAAGa > 1:953363/1‑69 (MQ=255) ccTGATGGCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATAAGa < 1:496882/68‑1 (MQ=255) ccTGATGGCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATAAGa < 1:1213186/68‑1 (MQ=255) ccTGATGGCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATAAGa < 1:3054388/68‑1 (MQ=255) cTGATGGCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGaaa > 1:2347130/1‑69 (MQ=255) cTGATGGCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGaaa > 1:2492796/1‑69 (MQ=255) cTGATGGCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGaaa > 1:409674/1‑69 (MQ=255) cTGATGGCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGaaa > 1:3133636/1‑69 (MQ=255) cTGATGGCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGaaa > 1:1542782/1‑69 (MQ=255) cTGATGGCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGaaa > 1:933501/1‑69 (MQ=255) cTGATGGCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGaaa > 1:3194405/1‑69 (MQ=255) cTGATGGCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGaaa > 1:1377312/1‑69 (MQ=255) cTGATGGCGCACCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGaaa > 1:3289531/1‑69 (MQ=255) ggCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGCCCACTCTTTCGACGGCATCAGAAacacac > 1:3281771/1‑69 (MQ=255) ggCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAacacac > 1:2981326/1‑69 (MQ=255) ggCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAacacac > 1:2474068/1‑69 (MQ=255) ggCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAacacac > 1:3271532/1‑69 (MQ=255) ggCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAacacac > 1:1867556/1‑69 (MQ=255) gcgcCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACtt > 1:3010345/1‑68 (MQ=255) gcCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTcc < 1:1380789/69‑1 (MQ=255) ccACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTcc < 1:1333207/68‑1 (MQ=255) ccACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTcc < 1:1088758/68‑1 (MQ=255) ctTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTcc > 1:2161818/1‑62 (MQ=255) ctTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTcc > 1:409788/1‑62 (MQ=255) ctTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTcc > 1:1397996/1‑62 (MQ=255) ctTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTcc > 1:3145191/1‑62 (MQ=255) ctTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTcc > 1:516815/1‑62 (MQ=255) ctTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTcc > 1:853477/1‑62 (MQ=255) ctTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTCCAGTTcc > 1:3280778/1‑68 (MQ=255) tCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTCCAGt > 1:3180892/1‑62 (MQ=255) tCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGc > 1:630961/1‑68 (MQ=255) aaCGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAg > 1:452059/1‑69 (MQ=255) aCGATGCAGAGATAAACGACCACTCTTTCGACGGCATAAGAAACACACTTTCCAGTTCCGGCATTAAGt > 1:436708/1‑69 (MQ=255) aCGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAg > 1:2776664/1‑68 (MQ=255) aCGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAg > 1:2138776/1‑68 (MQ=255) aCGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAGt > 1:928910/1‑69 (MQ=255) aCGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAGt > 1:108806/1‑69 (MQ=255) aCGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAGt > 1:196934/1‑69 (MQ=255) aCGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAGt > 1:1127299/1‑69 (MQ=255) aCGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAGt > 1:1425807/1‑69 (MQ=255) aCGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAGt > 1:1774427/1‑69 (MQ=255) aCGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAGt > 1:1778407/1‑69 (MQ=255) aCGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAGt > 1:2011377/1‑69 (MQ=255) aCGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAGt > 1:2402019/1‑69 (MQ=255) aCGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAGt > 1:2781015/1‑69 (MQ=255) gAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAGTGGCGa > 1:797363/1‑69 (MQ=255) gAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAGTGGCGa > 1:1747060/1‑69 (MQ=255) aaGAGATAAACGACCACTCTTTCGACGGCATAAGa < 1:1901570/35‑1 (MQ=255) tAAACGACCACTCTTTCGACGGCATCAGAAACACACTATCCAGTTCCGGCATTTAGTGGCGATTCata < 1:182108/68‑1 (MQ=255) ctctTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAGTGGCGATTCATATGCGCCAGCTg < 1:2291948/69‑1 (MQ=255) ctctTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAGTGGCGATTCATATGCGCCAGCTg < 1:2290802/69‑1 (MQ=255) ctctTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAGTGGCGATTCATATGCGCCAGCTg < 1:2790332/69‑1 (MQ=255) ctctTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAGTGGCGATTCATATGCGCCAGCTg < 1:641499/69‑1 (MQ=255) ctctTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAGTGGCGATTCATATGCGCCAGCTg < 1:2528861/69‑1 (MQ=255) ctctTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAGTGGCGATTCATATGCGCCAGCTg < 1:3398419/69‑1 (MQ=255) ggCATCAGAAACACACTTTCCAGTTCCGGCATTAAGTGGCGATTCATATGCGCCAGCTGCATTTCata > 1:2714911/1‑68 (MQ=255) | CGCCCTGATGGCGCGCCACCTCTTTCACCAACGATGAAGAGATAAACGACCACTCTTTCGACGGCATCAGAAACACACTTTCCAGTTCCGGCATTAAGTGGCGATTCATATGCGCCAGCTGCATTTCATA > minE/2531230‑2531359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |