| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,546,268 | A→G | 39.5% | S93S (TCT→TCC) | rfaF ← | ADP‑heptose:LPS heptosyltransferase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,546,268 | 0 | A | G | 39.5% | 36.0 / 55.2 | 81 | S93S (TCT→TCC) | rfaF | ADP‑heptose:LPS heptosyltransferase II |
| Reads supporting (aligned to +/- strand): ref base A (33/16); new base G (20/12); total (53/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.62e-01 | |||||||||||
CATCTCGCCGCGCCAGCCGGTGCGATGAGGAATACCCGCGAAGAAAGGCACTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACGCAGGCTATGACCCAGTT > minE/2546211‑2546335 | cATCTCGCCGCGCCAGCCGGTGCGATGAGGAATACCCGCGAAGAAAGGCACTAATGCAGATTTGAAGGa > 1:3010507/1‑69 (MQ=255)cATCTCGCCGCGCCAGCCGGTGCGATGAGGAATACCCGCGAAGAAAGGCACTAATGCAGATTTGAAGGa < 1:2672583/69‑1 (MQ=255)cATCTCGCCGCGCCAGCCGGTGCGATGAGGAATACCCGCGAAGAAAGGCACTAATGCAGATTTGAAGGa > 1:3337014/1‑69 (MQ=255) gcgcCAGCCGGTGCGATGAGGAATACCCGCGAAGAAAGGCACTAATGCAGATTTGAAGGAGTTGGGTaa < 1:3280237/69‑1 (MQ=255) cgcCAGCCGGTGCGATGAGGAATACCCGCGAAGAAAGGCACTAATGCAGATTTGAAGGAGTTGGGTaa < 1:566884/68‑1 (MQ=255) cgcCAGCCGGTGCGATGAGGAATACCCGCGAAGAAAGGCACTAATGCAGATTTGAAGGAGTTGGGTaa < 1:1359888/68‑1 (MQ=255) cgcCAGCCGGTGCGATGAGGAATACCCGCGAAGAAAGGCACTAATGCAGATTTGAAGGAGTTGGGTaa < 1:764030/68‑1 (MQ=255) cGATGAGGAATACCTGCGAAGAAAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCgg < 1:2495443/69‑1 (MQ=255) cGATGAGGAATACCTGCGAAGAAAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCgg < 1:2067576/69‑1 (MQ=255) cGATGAGGAATACCTGCGAAGAAAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCgg < 1:2282985/69‑1 (MQ=255) cGATGAGGAATACCTGCGAAGAAAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCgg < 1:2304610/69‑1 (MQ=255) cGATGAGGAATACCTGCGAAGAAAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCgg < 1:444313/69‑1 (MQ=255) cGATGAGGAATACCTGCGAAGAAAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCgg < 1:1672431/69‑1 (MQ=255) cGATGAGGAATACCTGCGAAGAAAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCgg < 1:364733/69‑1 (MQ=255) cGATGAGGAATACCTGCGAAGAAAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCgg < 1:3141597/69‑1 (MQ=255) cGATGAGGAATACCTGCGAAGAAAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCgg < 1:1438866/69‑1 (MQ=255) cGATGAGGAATACCTGCGAAGAAAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCgg < 1:2963775/69‑1 (MQ=255) cGATGAGGAATACCTGCGAAGAAAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCgg < 1:1319167/69‑1 (MQ=255) cGATGAGGAATACCTGCGAAGAAAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCgg < 1:3198593/69‑1 (MQ=255) cGATGAGGAATACCCGCGAAGAAAGGCACTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCgg > 1:2260415/1‑69 (MQ=255) cGATGAGGAATACCCGCGAAGAAAGGCACTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCgg > 1:1230937/1‑69 (MQ=255) aagaaAGGCACTAATGCGGATTTGAAGGAGTTAGGt > 1:1005668/1‑36 (MQ=37) aagaaAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCGGTCg > 1:725203/1‑54 (MQ=255) aagaaAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCGGTCg > 1:1330160/1‑54 (MQ=255) aagaaAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCGGTCg > 1:284598/1‑54 (MQ=255) aagaaAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCGGTCg > 1:1348814/1‑54 (MQ=255) aagaaAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCGGTCg > 1:2791755/1‑54 (MQ=255) aagaaAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCGGTCg > 1:833241/1‑54 (MQ=255) aagaaAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCGGTCg > 1:88031/1‑54 (MQ=255) aagaaAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCGGTCg > 1:926960/1‑54 (MQ=255) aagaaAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCGGTCg > 1:158937/1‑54 (MQ=255) aagaaAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCGGTCg > 1:1619191/1‑54 (MQ=255) aagaaAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCGGTCg > 1:699127/1‑54 (MQ=255) aagaaAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCGGTCg > 1:1917978/1‑54 (MQ=255) aagaaAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCGGTCg > 1:2203896/1‑54 (MQ=255) aagaaAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCGGTCg > 1:1156576/1‑54 (MQ=255) aagaaAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCGGTCg > 1:1974624/1‑54 (MQ=255) aagaaAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCGGTCg > 1:533359/1‑54 (MQ=255) aagaaAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCGGTCg > 1:2054081/1‑54 (MQ=255) aagaaAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCGGTCg > 1:2044317/1‑54 (MQ=255) aagaaAGGCACTAATGCGGATTTGAAGGAGTTAGGTAAGACGTAGGCGCGGTCg > 1:1999762/1‑54 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGTTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:2684642/1‑59 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:1070667/1‑59 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg < 1:3028320/59‑1 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg < 1:3216906/59‑1 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:3402219/1‑59 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:3429566/1‑59 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg < 1:359074/59‑1 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg < 1:624099/59‑1 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:496065/1‑59 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:499897/1‑59 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:1646361/1‑59 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:2123785/1‑59 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:193456/1‑59 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:2380837/1‑59 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:1735420/1‑59 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:2649284/1‑59 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:2703432/1‑59 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:2804313/1‑59 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:1716011/1‑59 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:2871446/1‑59 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:288970/1‑59 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:2913724/1‑59 (MQ=255) aCTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:2949247/1‑59 (MQ=255) tAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGgcg < 1:1162615/37‑1 (MQ=255) aaTGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg < 1:3044803/56‑1 (MQ=255) aaTGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:1582359/1‑56 (MQ=255) aaTGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:1235809/1‑56 (MQ=255) aaTGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg < 1:1778541/56‑1 (MQ=255) aaTGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg < 1:957984/56‑1 (MQ=255) aaTGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg < 1:3301110/56‑1 (MQ=255) aaTGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg < 1:325003/56‑1 (MQ=255) aaTGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACg > 1:3129661/1‑56 (MQ=255) aaTGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACGCAGGCTATGAccc > 1:3235742/1‑69 (MQ=255) tGCAGATTTGAATGAGTTGGGTAAGACGTATGAGCGGTCGTAGCGCTTTTCACg < 1:982532/54‑1 (MQ=255) cAGATTTGAAGGAGTTGGGTAAGACGTAGTCGCGGTCGTAgcgc > 1:2139303/1‑44 (MQ=255) cAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACGCAGGCTATGACCCAGtt > 1:1938144/1‑69 (MQ=255) cAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACGCAGGCTATGACCCAGtt > 1:2366402/1‑69 (MQ=255) cAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACGCAGGCTATGACCCAGtt > 1:2556052/1‑69 (MQ=255) cAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACGCAGGCTATGACCCAGtt > 1:2866412/1‑69 (MQ=255) cAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACGCAGGCTATGACCCAGtt > 1:3242670/1‑69 (MQ=255) | CATCTCGCCGCGCCAGCCGGTGCGATGAGGAATACCCGCGAAGAAAGGCACTAATGCAGATTTGAAGGAGTTGGGTAAGACGTAGGCGCGGTCGTAGCGCTTTTCACGCAGGCTATGACCCAGTT > minE/2546211‑2546335 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |