| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 27,949 | G→T | 80.9% | R207R (CGG→CGT) | carA → | carbamoyl phosphate synthetase small subunit, glutamine amidotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 27,949 | 0 | G | T | 80.9% | 114.8 / 23.4 | 68 | R207R (CGG→CGT) | carA | carbamoyl phosphate synthetase small subunit, glutamine amidotransferase |
| Reads supporting (aligned to +/- strand): ref base G (8/5); new base T (37/18); total (45/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
CGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTG > minE/27900‑28012 | cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGc > 1:905167/1‑68 (MQ=255)cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:1226766/1‑69 (MQ=255)cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:1341460/1‑69 (MQ=255)cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:381746/1‑69 (MQ=255)cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:2306908/1‑69 (MQ=255)cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:1257741/1‑69 (MQ=255)cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:2418182/1‑69 (MQ=255)cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:2095878/1‑69 (MQ=255)cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:3312210/1‑69 (MQ=255)cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:118354/1‑69 (MQ=255)cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:2080112/1‑69 (MQ=255)cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:2063700/1‑69 (MQ=255)cGTACCACGTCGTGGCCTACGATTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCt > 1:983571/1‑69 (MQ=255) tGGCTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGAcc < 1:1524151/68‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATTGTTcc < 1:536223/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:3159186/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:167098/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:2221872/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:241167/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:2855359/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:2481428/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:2570243/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:2585471/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:2621948/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:1978140/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:1791927/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:1172556/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:1547208/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:1277436/67‑1 (MQ=255) aTTTCGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:1255066/67‑1 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTTGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:2035946/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGAt > 1:1174494/1‑40 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:3174498/66‑1 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:2627558/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:937336/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:2695621/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:747106/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:738227/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:729380/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:3420754/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:1052809/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:3045109/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:305675/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:1866263/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:1326253/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:1332106/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:1107778/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:2626025/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:1066236/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:203827/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:2042529/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:2450550/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCAgg > 1:1669489/1‑68 (MQ=255) tttcGGTGCCAAGCGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTAGCCTGACCATCGTTCCgg > 1:987166/1‑68 (MQ=255) tttcGGTGCCAAGAGCAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCgg > 1:122607/1‑68 (MQ=255) gcgcAACATCCTGCGTATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTcc < 1:2728613/54‑1 (MQ=255) cgcAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTgt > 1:1008976/1‑67 (MQ=255) cgcAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTgt > 1:201516/1‑67 (MQ=255) cgcAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTgt > 1:1451109/1‑67 (MQ=255) cgcAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTgt > 1:408104/1‑67 (MQ=255) aaCATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGaa < 1:1337934/69‑1 (MQ=255) aaCATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGaa < 1:432046/69‑1 (MQ=255) aaCATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGaa < 1:2547485/69‑1 (MQ=255) aCATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGaa < 1:3151604/68‑1 (MQ=255) tCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGAtg > 1:1237943/1‑69 (MQ=255) tCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGAtg > 1:685218/1‑69 (MQ=255) cTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGAtgtg > 1:699344/1‑69 (MQ=255) cTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGAtgtg > 1:3086635/1‑69 (MQ=255) | CGTTCCACGTCGTGGCTTATGATTTTGGTGCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCGGCGCAAACTTCTGCGGAAGATGTG > minE/27900‑28012 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |