| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 218,450 | C→T | 26.4% | V188V (GTG→GTA) | yafV ← | predicted C‑N hydrolase family amidase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 218,450 | 0 | C | T | 26.4% | 54.4 / 15.7 | 49 | V188V (GTG→GTA) | yafV | predicted C‑N hydrolase family amidase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (22/14); new base T (5/8); total (27/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01 | |||||||||||
ATCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGG > minE/218385‑218512 | aTCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCcaca > 1:2335597/1‑69 (MQ=255)aTCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCcaca > 1:413118/1‑69 (MQ=255)aTCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCcaca > 1:907866/1‑69 (MQ=255)aTCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCcaca > 1:2651595/1‑69 (MQ=255) tCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCcaca < 1:804981/68‑1 (MQ=255) aaTGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCTACATACGTCTGATTCTCAATcgcgc > 1:242187/1‑69 (MQ=255) aaTGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCTACATACGTCTGATTCTCAATcgcgc > 1:935476/1‑69 (MQ=255) aaTGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCTACATACGTCTGATTCTCAATcgcgc > 1:935116/1‑69 (MQ=255) aaTGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCTACATACGTCTGATTCTCAATcgcgc > 1:3127260/1‑69 (MQ=255) aaTGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCTACATACGTCTGATTATCAATcgcgc > 1:681695/1‑69 (MQ=255) gCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATtctc > 1:2432832/1‑57 (MQ=255) gCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATtctc > 1:370127/1‑57 (MQ=255) gCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATtctc > 1:471631/1‑57 (MQ=255) gCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATtctc > 1:1643731/1‑57 (MQ=255) gCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATtctc > 1:92414/1‑57 (MQ=255) gCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATtctc > 1:1392255/1‑57 (MQ=255) gCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATtctc > 1:110981/1‑57 (MQ=255) gCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATtctc > 1:107064/1‑57 (MQ=255) gCAGCCGTTGCCATCGCTGCCGACGCGATTGCATACCGCCACATACGCCTGATtctc > 1:3130115/1‑57 (MQ=255) gCAGCCGTTGCCATCG‑‑GCCGACGCGATTGCATCCCGCCACATACGCCTGATtctc > 1:3333230/1‑55 (MQ=255) gTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAg < 1:1814653/69‑1 (MQ=255) gTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAg < 1:352880/69‑1 (MQ=255) ttGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGc < 1:2756266/69‑1 (MQ=255) ttGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGc < 1:2545246/69‑1 (MQ=255) ttGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGc < 1:2124375/69‑1 (MQ=255) ttGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGc < 1:3145941/69‑1 (MQ=255) ttGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGc < 1:1816085/69‑1 (MQ=255) gttccATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGc < 1:1832120/66‑1 (MQ=255) gCCATCGCTGCCGACGCGATTGCATCCCGCTACATACGTCTGATTCTCAATCGCGCGGGCCGTCAGCat < 1:2387548/69‑2 (MQ=255) gCCATCGCTGCCGACGCGATTGCATCCCGCTACATACGTCTGATTCTCAATCGCGCGGGCCGTCAGCaa < 1:2586860/69‑1 (MQ=255) gCCATCGCTGCCGACGCGATTGCATCCCGCTACATACGTCTGATTCTCAATCGCGCGGGCCGTCAGCaa < 1:97370/69‑1 (MQ=255) gCCATCGCTGCCGACGCGATTGCATCCCGCTACATACGTCTGATTCTCAATCGCGCGGGCCGTCAGCaa < 1:2949390/69‑1 (MQ=255) gCCATCGCTGCCGACGCGATTGCATCCCGCTACATACGTCTGATTCTCAATCGCGCGGGCCGTCAGCaa < 1:2254609/69‑1 (MQ=255) gCCATCGCTGCCGACGCGATTGCATCCCGCTACATACGTCTGATTCTCAATCGCGCGGGCCGTCAGCaa < 1:1585186/69‑1 (MQ=255) gCCATCGCTGCCGACGCGATTGCATCCCGCTACATACGTCTGATTCTCAATCGCGCGGGCCGTCAGCaa < 1:203660/69‑1 (MQ=255) cATCGCTGCCGACGCGATTGCATCCCGCTACATACGTCTGATTCTCAATCGCGCGGGCCGTCAGCaa < 1:3363340/67‑1 (MQ=255) cGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAAtgcct > 1:270197/1‑69 (MQ=255) cGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAAtgcct > 1:2332408/1‑69 (MQ=255) cGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAAtgcct > 1:2089563/1‑69 (MQ=255) cGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAAtgcc > 1:172949/1‑68 (MQ=255) gCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAAtgcct > 1:2757042/1‑68 (MQ=255) gCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAAtgcct > 1:1184784/1‑68 (MQ=255) gCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAAtgcct > 1:2223624/1‑68 (MQ=255) gCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAAtgcct > 1:478954/1‑68 (MQ=255) tGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAAtgcctgcc < 1:991211/69‑1 (MQ=255) tGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAAtgcctgcc < 1:119713/69‑1 (MQ=255) tGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAAtgcctgcc < 1:1533080/69‑1 (MQ=255) tGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAAtgcctgcc < 1:424266/69‑1 (MQ=255) tGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAAtgcctgcc < 1:2311301/69‑1 (MQ=255) tGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAAtgcctgcc < 1:2448645/69‑1 (MQ=255) tGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAgc > 1:124377/1‑69 (MQ=255) tGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAgc > 1:3072250/1‑69 (MQ=255) agCATCCCGCCACATACGCCTGATTCTCAATCGCGCggg > 1:616046/2‑39 (MQ=255) ccGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCgg > 1:1013467/1‑67 (MQ=255) | ATCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGG > minE/218385‑218512 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |