| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,547,168 | A→G | 51.8% | R107R (CGT→CGC) | rfaD ← | ADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,547,168 | 0 | A | G | 51.8% | 0.9 / 49.0 | 58 | R107R (CGT→CGC) | rfaD | ADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (11/17); new base G (15/15); total (26/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 | |||||||||||
TCGGAGGTGCGTCCGCCGTAGGTGGCTGCGGAAGAAGCGTACAGGAACGGGATTTCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCCATCATATACTTGCC > minE/2547112‑2547233 | tCGGAGGTGCGTCCACCGTAAGTGGCTGCGGAAGAGTCATACAGGAACGGGATTTCGCGCTCCAGACag < 1:2491031/69‑1 (MQ=255)tCGGAGGTGCGTCCACCGTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACag < 1:1952763/69‑1 (MQ=255)tCGGAGGTGCGTCCACCGTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACag < 1:436099/69‑1 (MQ=255)tCGGAGGTGCGTCCACCGTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACag < 1:1240843/69‑1 (MQ=255)tCGGAGGTGCGTCCACCGTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACag < 1:2158555/69‑1 (MQ=255)tCGGAGGTGCGTCCACCGTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACag < 1:3244287/69‑1 (MQ=255)tCGGAGGTGCGTCCACCGTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACag < 1:3221200/69‑1 (MQ=255)tCGGAGGTGCGTCCACCGTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACag < 1:3140906/69‑1 (MQ=255)tCGGAGGTGCGTCCACCGTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACag < 1:2940758/69‑1 (MQ=255)tCGGAGGTGCGTCCACCGTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACag < 1:1761505/69‑1 (MQ=255)tCGGAGGTGCGTCCACCGTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACag < 1:165400/69‑1 (MQ=255)tCGGAGGTGCGTCCACCGTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACag < 1:2424628/69‑1 (MQ=255)tCGGAGGTGCGTCCACCGTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACag < 1:1720512/69‑1 (MQ=255)tCGGAGGTGCGTCCACCGTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACag < 1:2832122/69‑1 (MQ=255) cGGAGGTGCGTCCGCCGTAGGTGGCTGCGGAAGAAGCGTACAGGAACGGGATTTCACGCTCCAGGCagt < 1:184404/69‑1 (MQ=255) cGGAGGTGCGTCCGCCGTAGGTGGCTGCGGAAGAAGCGTACAGGAACGGGATTTCACGCTCCAGGCagt < 1:2874552/69‑1 (MQ=255) cgTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACAGTAGTGCAGCAGCt > 1:2390114/1‑66 (MQ=255) cgTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACAGTAGTGCAGCAGCTctt > 1:2366116/1‑67 (MQ=255) cgTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACAGTAGTGCAGCAGCTctt > 1:1093009/1‑67 (MQ=255) cgTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACAGTAGTGCAGCAGCTctt > 1:2836277/1‑67 (MQ=255) cgTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACAGTAGTGCAGCAGCTctt > 1:2272925/1‑67 (MQ=255) cgTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACAGTAGTGCAGCAGCTctt > 1:2369348/1‑67 (MQ=255) cgTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACAGTAGTGCAGCAGCTctt > 1:2854941/1‑67 (MQ=255) cgTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACAGTAGTGCAGCAGCTctt > 1:3385141/1‑67 (MQ=255) cgTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACAGTAGTGCAGCAGCTctt > 1:845923/1‑67 (MQ=255) cgTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACAGTAGTGCAGCAGCTctt > 1:1261791/1‑67 (MQ=255) cgTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACAGTAGTGCAGCAGCTctt > 1:1451928/1‑67 (MQ=255) cgTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACAGTAGTGCAGCAGCTctt > 1:193739/1‑67 (MQ=255) cgTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACAGTAGTGCAGCAGCTctt > 1:1877340/1‑67 (MQ=255) cgTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACAGTAGTGCAGCAGCTctt > 1:1591206/1‑67 (MQ=255) cgTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACAGTAGTGCAGCAGCTctt > 1:1758513/1‑67 (MQ=255) gTAAGTGGCTGCGGAAGAGGCATACAGGAACGGGATTTCGCGCTCCAGACag < 1:3080084/52‑1 (MQ=255) tGGCTGCGGAAGAAGCGTACAGGAACGGGATTTCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAg < 1:1990076/68‑1 (MQ=255) tGGCTGCGGAAGAAGCGTACAGGAACGGGATTTCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAg < 1:633679/68‑1 (MQ=255) tGGCTGCGGAAGAAGCGTACAGGAACGGGATTTCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAg < 1:2836113/68‑1 (MQ=255) aagCGTACAGGAACGGGATTTCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAgttgt < 1:2225749/69‑1 (MQ=255) aagCGTACAGGAACGGGATTTCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAgttgt < 1:2366955/69‑1 (MQ=255) agCGTACAGGAACGGGATTTCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGTTAgttgtt > 1:2856605/1‑69 (MQ=255) cAGGAACGGGATTTCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCcat < 1:2745271/69‑1 (MQ=255) cAGGAACGGGATTTCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCcat < 1:1827821/69‑1 (MQ=255) cAGGAACGGGATTTCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCcat < 1:1392791/69‑1 (MQ=255) aaCGGGATTTCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCCATCata < 1:2717631/69‑1 (MQ=255) aCGGGATTTCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCCATCatat > 1:2593705/1‑69 (MQ=255) aCGGGATTTCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCCATCatat > 1:3333919/1‑69 (MQ=255) aCGGGATTTCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCCATCatat > 1:2382824/1‑69 (MQ=255) aCGGGATTTCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCCATCatat > 1:2059504/1‑69 (MQ=255) aCGGGATTTCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCCATCatat > 1:3206058/1‑69 (MQ=255) aCGGGATTTCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCCATCata > 1:246756/1‑68 (MQ=255) aCGGGAATTCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCCATCatat > 1:1498/1‑69 (MQ=255) ttaaCGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCCATCATATACTTGcc < 1:2458903/66‑1 (MQ=255) ttCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGTGTATTGATAGTTGTTATCCATCATATACTTGcc > 1:123664/1‑69 (MQ=255) ttCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCCATCATATACTTGcc > 1:1102791/1‑69 (MQ=255) ttCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCCATCATATACTTGcc < 1:821785/69‑1 (MQ=255) ttCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCCATCATATACTTGcc < 1:2249835/69‑1 (MQ=255) ttCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCCATCATATACTTGcc > 1:413654/1‑69 (MQ=255) ttCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCCATCATATACTTGcc < 1:1404339/69‑1 (MQ=255) ttCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCCATCATATACTTGcc < 1:1471956/69‑1 (MQ=255) ttCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCCATCATATACTTGcc < 1:1675213/69‑1 (MQ=255) ttCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCCATCATATACTTGcc < 1:2679364/69‑1 (MQ=255) | TCGGAGGTGCGTCCGCCGTAGGTGGCTGCGGAAGAAGCGTACAGGAACGGGATTTCACGCTCCAGGCAGTAGTGCAGCAGCTCCTTGGAGTATTGATAGTTGTTATCCATCATATACTTGCC > minE/2547112‑2547233 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |