| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,554,362 | G→C | 52.7% | L92L (CTC→CTG) | envC ← | protease with a role in cell division |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,554,362 | 0 | G | C | 52.7% | 7.8 / 111.7 | 93 | L92L (CTC→CTG) | envC | protease with a role in cell division |
| Reads supporting (aligned to +/- strand): ref base G (27/17); new base C (18/31); total (45/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.27e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01 | |||||||||||
CCTGGGCGGCTTTTTGCTGCTCCAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCAGAGATCGCTTCTTC > minE/2554298‑2554424 | ccTGGGCGGCTTTTTGCTGCTCCAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTGAGtt > 1:2719460/1‑69 (MQ=255) gggCGGCTTTTTGCTGCTCCAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTGAGttg < 1:2279538/67‑1 (MQ=255) tgctCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGTTTTGc > 1:821932/1‑69 (MQ=255) tgctCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGTTTTGc > 1:1744151/1‑69 (MQ=255) tgctCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGTTTTGc > 1:19461/1‑69 (MQ=255) tgctCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGTTTTGc > 1:1983639/1‑69 (MQ=255) tgctCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGTTTTGc > 1:2706489/1‑69 (MQ=255) tgctCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGTTTTGc > 1:2670687/1‑69 (MQ=255) tgctCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGTTTTGc > 1:3147799/1‑69 (MQ=255) tgctCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGTTTTGc > 1:2216472/1‑69 (MQ=255) tgctCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGTTTTGc > 1:2220498/1‑69 (MQ=255) tgctCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGTTTTGc > 1:344898/1‑69 (MQ=255) tgctCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGTTTTGc > 1:416736/1‑69 (MQ=255) tgctCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGTTTTGc > 1:2274620/1‑69 (MQ=255) tgctCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGTTTTGc > 1:1202171/1‑69 (MQ=255) tgctCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGTTTTGc > 1:1186044/1‑69 (MQ=255) tgctCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGTTTTGc > 1:1167499/1‑69 (MQ=255) tgctCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGTTTTGc > 1:44421/1‑69 (MQ=255) tgctCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGTTTTGc > 1:2308866/1‑69 (MQ=255) tgctCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGTTTTGc > 1:234418/1‑69 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:2468640/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:2565059/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:2282230/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:2136500/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:2408550/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:1888388/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:246307/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:1873859/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:1754142/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:609840/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:58978/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:575225/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:1169026/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:1212643/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:1300762/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:1304659/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:1450366/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:1456388/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:3413859/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:2961031/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:2804135/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:2797881/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:274140/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:1743104/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:1773414/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTCttt < 1:203316/57‑1 (MQ=255) ttGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATCAAGCGTGttt < 1:1681677/57‑1 (MQ=255) cAATCGACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:1689299/53‑1 (MQ=255) gACGCGTTCATCTCATCAATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACgg > 1:972738/1‑69 (MQ=255) gACGCGTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:3120414/48‑1 (MQ=255) aCGCTTTCATCTCATCAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:2743124/47‑1 (MQ=255) gcgTTCATCTCATCAATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTg < 1:882413/69‑1 (MQ=255) gcgTTCATCTCATCAATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTg < 1:2505200/69‑1 (MQ=255) gcgTTCATCTCATCAATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTg < 1:685408/69‑1 (MQ=255) atctcatcAATCTGTTTATTCAGTTGATTAAGCGTGttt < 1:2553423/39‑1 (MQ=38) tcatcaATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTa < 1:1031950/54‑1 (MQ=255) tcatcaATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTa > 1:1415883/1‑54 (MQ=255) tcatcaATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTa > 1:1919689/1‑54 (MQ=255) tcatcaATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTa < 1:1614813/54‑1 (MQ=255) tcatcaATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCtt > 1:2181536/1‑64 (MQ=255) tcatcaATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCtt > 1:872662/1‑64 (MQ=255) tcatcaATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCtt > 1:458189/1‑64 (MQ=255) tcatcaATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCtt > 1:1384327/1‑64 (MQ=255) tcatcaATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCtt > 1:1498901/1‑64 (MQ=255) tcatcaATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCt > 1:1019603/1‑63 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCt > 1:632526/1‑48 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCt > 1:1037842/1‑48 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCt > 1:1068434/1‑48 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCt > 1:1080727/1‑48 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCt > 1:536048/1‑48 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCt > 1:2671647/1‑48 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCt > 1:2084783/1‑48 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCt > 1:1478414/1‑48 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCt > 1:2609846/1‑48 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCt > 1:824117/1‑48 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCt > 1:1658310/1‑48 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCt > 1:3107578/1‑48 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCt > 1:2245262/1‑48 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCt > 1:898896/1‑48 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCt > 1:950805/1‑48 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACg > 1:2110981/1‑52 (MQ=255) caATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCAGAGATCg > 1:112969/1‑69 (MQ=255) tCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCAGAGATCGct < 1:2450087/68‑1 (MQ=255) tATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCAGAGATCGcttct < 1:3414835/65‑1 (MQ=255) tATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCAGAGATCGcttct < 1:2652252/65‑1 (MQ=255) tATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCAGAGATCGcttct < 1:3371581/65‑1 (MQ=255) tATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCAGAGATCGcttct < 1:823068/65‑1 (MQ=255) tATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCAGAGATCGcttct < 1:2733377/65‑1 (MQ=255) tATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCAGAGATCGcttct < 1:2597466/65‑1 (MQ=255) tATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCAGAGATCGcttct < 1:1610464/65‑1 (MQ=255) tATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCAGAGATCGcttct < 1:2940920/65‑1 (MQ=255) tATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCAGAGATCGcttct < 1:1638298/65‑1 (MQ=255) tATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCAGAGATCGCttcttc < 1:2846301/67‑1 (MQ=255) | CCTGGGCGGCTTTTTGCTGCTCCAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTTTATTGAGTTGATTGAGCGTGTTTTGCGTTTCGCGCAGCTTACGGGTGGCTTCAGAGATCGCTTCTTC > minE/2554298‑2554424 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |