| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,555,880 | C→T | 53.6% | A112A (GCG→GCA) | gpmI ← | phosphoglycero mutase III, cofactor‑independent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,555,880 | 0 | C | T | 53.6% | 15.1 / 121.3 | 138 | A112A (GCG→GCA) | gpmI | phosphoglycero mutase III, cofactor‑independent |
| Reads supporting (aligned to +/- strand): ref base C (54/10); new base T (14/60); total (68/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.34e-15 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 138 total aligned reads displayed.
ATCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGCGCCAGTCAGCACCGGATTAGCAAAGAAAGCGCG > minE/2555815‑2555945 | aTCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCt > 1:2474193/1‑69 (MQ=255)aTCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCt > 1:1601251/1‑69 (MQ=255)aTCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCt > 1:822425/1‑69 (MQ=255)aTCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCt > 1:912633/1‑69 (MQ=255)aTCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCt > 1:2557456/1‑69 (MQ=255)aTCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCt > 1:2269024/1‑69 (MQ=255) gCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTg > 1:3425106/1‑69 (MQ=255) atgTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCg > 1:75220/1‑69 (MQ=255) atgTGATCTTCGTGGCTGTGTACACCACCTGCGGAGAGCATACCCATAATGTGTACTGCttt < 1:3203661/62‑1 (MQ=255) atgTGATCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCttt < 1:1033131/62‑1 (MQ=255) atgTGATCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCttt < 1:1208095/62‑1 (MQ=255) atgTGATCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCttt < 1:37967/62‑1 (MQ=255) atgTGATCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCttt < 1:1057093/62‑1 (MQ=255) atgTGATCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCttt < 1:1479397/62‑1 (MQ=255) atgTGATCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCttt < 1:2333540/62‑1 (MQ=255) atgTGATCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCttt < 1:1488432/62‑1 (MQ=255) atgTGATCATCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCg > 1:1836677/1‑69 (MQ=255) tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:2326685/1‑69 (MQ=255) tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:835176/1‑69 (MQ=255) tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:507142/1‑69 (MQ=255) tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:1918919/1‑69 (MQ=255) tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:2893992/1‑69 (MQ=255) tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:2941956/1‑69 (MQ=255) tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:3395778/1‑69 (MQ=255) tCTTCGTGGCTGTGTACACCACCTGCGGAGAGCAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTc > 1:1237611/1‑69 (MQ=255) ttCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGc > 1:1714229/1‑69 (MQ=255) tGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGc < 1:1616458/65‑1 (MQ=255) tGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGc < 1:1221931/65‑1 (MQ=255) tGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGc < 1:668923/65‑1 (MQ=255) tGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGc < 1:2005982/65‑1 (MQ=255) tGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGc < 1:2490204/65‑1 (MQ=255) tGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGc < 1:1317605/65‑1 (MQ=255) tACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGttt > 1:2128197/1‑52 (MQ=255) tACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcgc > 1:1188843/1‑69 (MQ=255) tACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcgc > 1:3259122/1‑69 (MQ=255) tACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcgc > 1:682528/1‑69 (MQ=255) tACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcgc > 1:177931/1‑69 (MQ=255) tACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcgc > 1:444996/1‑69 (MQ=255) tACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcgc > 1:2904147/1‑69 (MQ=255) cgccgcCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTAt > 1:1569668/1‑60 (MQ=255) cgccgcCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcgc > 1:3147040/1‑67 (MQ=255) cgccgcCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACcg > 1:438403/1‑66 (MQ=255) gagCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGCGCCAGTCAGCACCgg < 1:2034900/69‑1 (MQ=255) gagCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGCGCCAGTCAGCACCgg < 1:2478305/69‑1 (MQ=255) cAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGAtt < 1:1204725/69‑1 (MQ=255) cAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGAtt < 1:2881997/69‑1 (MQ=255) cAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGAtt < 1:3203421/69‑1 (MQ=255) cAGACCCATAATGTGTACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGAtt < 1:1647969/69‑1 (MQ=255) gACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGCGCCAGTCAGCACCGGATTa > 1:2664335/1‑68 (MQ=255) cccATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGCGCCAGTCAGCACCGGATTAg > 1:2842639/1‑67 (MQ=255) cccATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGCGCCAGTCAGCACCGGATTAg > 1:783604/1‑67 (MQ=255) cccATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGCGCCAGTCAGCACCGGATTAg > 1:690887/1‑67 (MQ=255) cccATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGCGCCAGTCAGCACCGGATTAg > 1:2886792/1‑67 (MQ=255) cccATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATATACCGCGCAAGTCAGCACCGGATTAg > 1:445557/1‑67 (MQ=255) aTGTGTACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTATCaaaga < 1:1372314/67‑1 (MQ=255) aTGTGTACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCaaaga < 1:1994598/67‑1 (MQ=255) aTGTGTACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCaaaga < 1:1408036/67‑1 (MQ=255) aTGTGTACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCaaaga < 1:2444727/67‑1 (MQ=255) aTGTGTACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCaaaga < 1:3168373/67‑1 (MQ=255) aTGTGTACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCaaaga < 1:500016/67‑1 (MQ=255) aTGTGTACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCaaaga < 1:1560707/67‑1 (MQ=255) aTGTGTACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCaaaga < 1:190280/67‑1 (MQ=255) aTGTGTACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCaaaga < 1:1297029/67‑1 (MQ=255) aTGTGTACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCaaaga < 1:2075479/67‑1 (MQ=255) aTGTGTACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCaaaga < 1:3224939/67‑1 (MQ=255) aTGTGTACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCaaaga < 1:3235367/67‑1 (MQ=255) aTGTGTACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCaaaga < 1:2137951/67‑1 (MQ=255) aTGTGTACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCaaaga < 1:2255354/67‑1 (MQ=255) aTGTGTACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCaaaga < 1:1231742/67‑1 (MQ=255) aTGTGTACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCaaaga < 1:1069560/67‑1 (MQ=255) aTGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGCGCCAg > 1:3024574/1‑46 (MQ=255) aTGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGCGCCAGTCAGCACCGGATTAGCaaagaaa > 1:191020/1‑69 (MQ=255) aTGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGCGCCAGTCAGCACCGGATTAGCaaagaaa > 1:2743469/1‑69 (MQ=255) aTGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGCGCCAGTCAGCACCGGATTAGCaaagaaa > 1:211596/1‑69 (MQ=255) aTGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGCGCCAGTCAGCACCGGATTAGCaaagaaa > 1:512871/1‑69 (MQ=255) aTGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGCGCCAGTCAGCACCGGATTAGCaaagaaa > 1:1039693/1‑69 (MQ=255) aTGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGCGCCAGTCAGCACCGGATTAGCaaagaaa > 1:464660/1‑69 (MQ=255) aTGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGCGCCAGTCAGCACCGGATTAGCaaagaaa > 1:2256595/1‑69 (MQ=255) aTGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGCGCCAGTCAGCACCGGATTAGCaaagaa > 1:1487678/1‑68 (MQ=255) gtgtACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGAtt < 1:1052270/57‑1 (MQ=255) tgtACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGCGCCAGTCAGc > 1:1195758/1‑48 (MQ=255) tACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCAAAGAAAgctc < 1:3117798/68‑3 (MQ=255) tACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCAAAGAAAgcgc < 1:991614/68‑1 (MQ=255) tACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCAAAGAAAgcgc < 1:470184/68‑1 (MQ=255) tACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCAAAGAAAgcgc < 1:993177/68‑1 (MQ=255) tACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCAAAGAAAgcgc < 1:2945740/68‑1 (MQ=255) tACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCAAAGAAAgcgc < 1:2707706/68‑1 (MQ=255) tACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCAAAGAAAgcgc < 1:2526846/68‑1 (MQ=255) tACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCAAAGAAAgcgc < 1:2451920/68‑1 (MQ=255) tACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCAAAGAAAgcgc < 1:2375022/68‑1 (MQ=255) tACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCAAAGAAAgcgc < 1:2182014/68‑1 (MQ=255) tACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCAAAGAAAgcgc < 1:2118593/68‑1 (MQ=255) tACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCAAAGAAAgcgc < 1:2039419/68‑1 (MQ=255) tACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCAAAGAAAgcgc < 1:1814148/68‑1 (MQ=255) tACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCAAAGAAAgcgc < 1:1704065/68‑1 (MQ=255) tACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCAAAGAAAgcgc < 1:1370707/68‑1 (MQ=255) tACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCAAAGAAAgcgc < 1:1204703/68‑1 (MQ=255) tACTGCTTTGCCTGCGTTTTTCGCTTTATCTACTGCACCAGTCAACACCGGATTAGCAAAGAAAgcgc < 1:1099489/68‑1 (MQ=255) tACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGCGCCAGTCAGCACCGGATTAGCAAAGAAAgcgc > 1:3354249/1‑68 (MQ=255) tACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGCGCCAGTCAGCACCGGATTAGCAAAGAAAGcgcg > 1:340675/1‑69 (MQ=255) | ATCGCCATGATGTGATCTTCGTGGCTGTGTACGCCGCCTGCGGAGAGCAGACCCATAATGTGTACCGCTTTGCCTGCGTTTTTCGCTTTATCTACCGCGCCAGTCAGCACCGGATTAGCAAAGAAAGCGCG > minE/2555815‑2555945 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |