| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,556,062 | G→A | 27.9% | L52L (CTG→TTG) | gpmI ← | phosphoglycero mutase III, cofactor‑independent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,556,062 | 0 | G | A | 27.9% | 139.2 / 51.8 | 125 | L52L (CTG→TTG) | gpmI | phosphoglycero mutase III, cofactor‑independent |
| Reads supporting (aligned to +/- strand): ref base G (40/50); new base A (7/28); total (47/78) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.35e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 125 total aligned reads displayed.
GGCACCCAGGTTAACGTGGCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATCCATTACCGGGGTTTTAG > minE/2555997‑2556127 | ggcACCCAGGTTAACGTGGCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGAcc < 1:2128298/69‑1 (MQ=255)ggcACCCAGGTTAACGTGGCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGAcc < 1:3127461/69‑1 (MQ=255)ggcACCCAGGTTAACGTGGCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGAcc < 1:1251680/69‑1 (MQ=255)ggcACCCAGGTTAACGTGGCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGAcc < 1:383657/69‑1 (MQ=255) aCCCAGGTTAACGTGGCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGa < 1:3224584/69‑1 (MQ=255) aCCCAGGTTAACGTGGCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGa < 1:3220645/69‑1 (MQ=255) ggCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAggg < 1:546546/69‑1 (MQ=255) ggCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAggg < 1:3412995/69‑1 (MQ=255) ggCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAggg < 1:1484382/69‑1 (MQ=255) ggCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAggg < 1:3363033/69‑1 (MQ=255) ggCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAggg < 1:1982304/69‑1 (MQ=255) ggCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAggg < 1:2571122/69‑1 (MQ=255) ggCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAggg < 1:2781869/69‑1 (MQ=255) ggCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAggg < 1:2811691/69‑1 (MQ=255) ggCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAggg < 1:2922796/69‑1 (MQ=255) gCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAggg < 1:1020677/68‑1 (MQ=255) gCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAggg < 1:3358985/68‑1 (MQ=255) gCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGt > 1:439819/1‑69 (MQ=255) gCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGt > 1:2658587/1‑69 (MQ=255) gCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGt > 1:3290956/1‑69 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:2921573/67‑1 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:2821266/67‑1 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:949251/67‑1 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:3185947/67‑1 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:926513/67‑1 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:1156243/67‑1 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:2234571/67‑1 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:488810/67‑1 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:2052628/67‑1 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:1265203/67‑1 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:1904969/67‑1 (MQ=255) gAGTTACCCATCTGACGGGCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:1684523/67‑1 (MQ=255) aCCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTa > 1:2170516/1‑55 (MQ=255) aCCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTgg > 1:1382051/1‑68 (MQ=255) tGACGGTCAGGGAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAg > 1:1240647/1‑68 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGTAAGCGTCGATTAGGGTATGCGGACGATTGGCCca > 1:2150933/1‑65 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACg > 1:217991/1‑56 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCcaca > 1:357521/1‑67 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCca > 1:1706628/1‑65 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCca > 1:3229747/1‑65 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCca > 1:1513099/1‑65 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCca > 1:2908922/1‑65 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCca > 1:3382383/1‑65 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCca > 1:269218/1‑65 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCca > 1:2155364/1‑65 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAg > 1:3309125/1‑68 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAg > 1:1545932/1‑68 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAg > 1:2640976/1‑68 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAg > 1:2113321/1‑68 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAg > 1:2208030/1‑68 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAg > 1:2848839/1‑68 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTg > 1:867632/1‑60 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCcaca > 1:1829709/1‑67 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAg > 1:2414116/1‑68 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAg > 1:1817891/1‑68 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAg > 1:2753173/1‑68 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAg > 1:1833849/1‑68 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:1624832/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:69827/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:32827/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:158722/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:1300081/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:173103/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:614162/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:1451066/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:546337/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:384119/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:1291837/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:406537/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:1728870/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:3075527/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:313151/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:1214367/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:2252390/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:918672/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:2860366/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:2803702/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGCCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:284330/69‑1 (MQ=255) gcAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:292557/68‑1 (MQ=255) cAGGCAGACCGACTTCCAGACCGGAAGCGTCGTTTAggg < 1:2620230/39‑1 (MQ=255) cAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc > 1:545903/1‑68 (MQ=255) aGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:724766/63‑1 (MQ=255) aGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:2002584/63‑1 (MQ=255) aGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:2433407/63‑1 (MQ=255) aGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:456240/63‑1 (MQ=255) aGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:2575398/63‑1 (MQ=255) aGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:2616017/63‑1 (MQ=255) aGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:2805420/63‑1 (MQ=255) aGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:1806452/63‑1 (MQ=255) aGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:2929518/63‑1 (MQ=255) aGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:916566/63‑1 (MQ=255) gACCGACTTCCAGACCGGAAGCGTCTATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:2208505/62‑1 (MQ=255) tccGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTTGCCCACAGTGCATcc < 1:328185/60‑1 (MQ=255) cGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATCCATTACCgggg > 1:2058765/1‑69 (MQ=255) cGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATCCATTACCgggg > 1:2618778/1‑69 (MQ=255) aCTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATCCATTACCGGGGt > 1:445449/1‑68 (MQ=255) aCTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATCCATTACCGGGGt > 1:3057291/1‑68 (MQ=255) ccAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATCCATTACCGGGGTTTTAg > 1:861566/1‑69 (MQ=255) ccAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATCCATTACCGGGGTTTTAg > 1:1769063/1‑69 (MQ=255) ccAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATCCATTACCGGGGTTTTAg > 1:1968837/1‑69 (MQ=255) | GGCACCCAGGTTAACGTGGCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATCCATTACCGGGGTTTTAG > minE/2555997‑2556127 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |