| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,556,078 | T→C | 26.8% | L46L (CTA→CTG) | gpmI ← | phosphoglycero mutase III, cofactor‑independent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,556,078 | 0 | T | C | 26.8% | 148.5 / 55.5 | 134 | L46L (CTA→CTG) | gpmI | phosphoglycero mutase III, cofactor‑independent |
| Reads supporting (aligned to +/- strand): ref base T (40/58); new base C (7/29); total (47/87) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 134 total aligned reads displayed.
GGCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATCCATTACCGGGGTTTTAG > minE/2556014‑2556127 | ggCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAggg < 1:1982304/69‑1 (MQ=255)ggCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAggg < 1:546546/69‑1 (MQ=255)ggCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAggg < 1:2571122/69‑1 (MQ=255)ggCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAggg < 1:2781869/69‑1 (MQ=255)ggCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAggg < 1:3412995/69‑1 (MQ=255)ggCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAggg < 1:2811691/69‑1 (MQ=255)ggCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAggg < 1:2922796/69‑1 (MQ=255)ggCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAggg < 1:1484382/69‑1 (MQ=255) gCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAggg < 1:1020677/68‑1 (MQ=255) gCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAggg < 1:3358985/68‑1 (MQ=255) gCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGt > 1:3171554/1‑69 (MQ=255) gCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGt > 1:2658587/1‑69 (MQ=255) gCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGt > 1:1514013/1‑69 (MQ=255) gCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGt > 1:3290956/1‑69 (MQ=255) gCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGt > 1:439819/1‑69 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:949251/67‑1 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:3185947/67‑1 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:2921573/67‑1 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:2834114/67‑1 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:2821266/67‑1 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:488810/67‑1 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:498097/67‑1 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:2234571/67‑1 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:2052628/67‑1 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:926513/67‑1 (MQ=255) gAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:1904969/67‑1 (MQ=255) gAGTTACCCATCTGACGGGCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGAc < 1:1684523/67‑1 (MQ=255) aCCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTa > 1:2170516/1‑55 (MQ=255) aCCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTgg > 1:1884393/1‑68 (MQ=255) aCCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTgg > 1:1382051/1‑68 (MQ=255) tGACGGTCAGGGAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAg > 1:1240647/1‑68 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGTAAGCGTCGATTAGGGTATGCGGACGATTGGCCca > 1:2150933/1‑65 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACg > 1:217991/1‑56 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCcaca > 1:357521/1‑67 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCca > 1:2908922/1‑65 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCca > 1:2798166/1‑65 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCca > 1:3229747/1‑65 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCca > 1:269218/1‑65 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCca > 1:3382383/1‑65 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCca > 1:2155364/1‑65 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAg > 1:2848839/1‑68 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAg > 1:1545932/1‑68 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAg > 1:2113321/1‑68 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAg > 1:2655701/1‑68 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTg > 1:867632/1‑60 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCcaca > 1:1829709/1‑67 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAg > 1:1817891/1‑68 (MQ=255) tGACGGTCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAg > 1:2414116/1‑68 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:69827/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:546337/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:32827/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:3315358/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:614162/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:411995/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:1214367/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:1451066/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:3075527/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:1300081/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:1291837/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:384119/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:1624832/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:1248115/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:2860366/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:1728870/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:2803702/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:173103/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:885452/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:2252390/69‑1 (MQ=255) tCAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:2338306/69‑1 (MQ=255) gcAGGCAGACCGACTTCCAAACCGGAAGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:292557/68‑1 (MQ=255) cAGGCAGACCGACTTCCAGACCGGAAGCGTCGTTTAggg < 1:2620230/39‑1 (MQ=255) aGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:1697359/63‑1 (MQ=255) aGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:456240/63‑1 (MQ=255) aGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:916566/63‑1 (MQ=255) aGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:2002584/63‑1 (MQ=255) aGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:2183383/63‑1 (MQ=255) aGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:2433407/63‑1 (MQ=255) aGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:1806452/63‑1 (MQ=255) aGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:2575398/63‑1 (MQ=255) aGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:724766/63‑1 (MQ=255) gACCGACTTCCAGACCGGAAGCGTCTATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:2208505/62‑1 (MQ=255) gACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATcc < 1:2607488/62‑1 (MQ=255) tccGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTTGCCCACAGTGCATcc < 1:328185/60‑1 (MQ=255) cGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATCCATTACCgggg > 1:2618778/1‑69 (MQ=255) cGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATCCATTACCgggg > 1:2058765/1‑69 (MQ=255) aCTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATCCATTACCGGGGt > 1:3057291/1‑68 (MQ=255) aCTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATCCATTACCGGGGt > 1:3056333/1‑68 (MQ=255) ccAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATCCATTACCGGGGTTTTAg > 1:1968837/1‑69 (MQ=255) ccAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATCCATTACCGGGGTTTTAg > 1:1769063/1‑69 (MQ=255) aaGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATc < 1:427457/43‑1 (MQ=255) aaGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATc < 1:39744/43‑1 (MQ=255) aaGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATc < 1:3392420/43‑1 (MQ=255) aaGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATc < 1:1326908/43‑1 (MQ=255) aaGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATc < 1:3310264/43‑1 (MQ=255) aaGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATc < 1:701520/43‑1 (MQ=255) aaGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATc < 1:1526636/43‑1 (MQ=255) aaGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATc < 1:2146148/43‑1 (MQ=255) aaGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATc < 1:1131900/43‑1 (MQ=255) aaGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATc < 1:1008052/43‑1 (MQ=255) aaGCGTCGATCAGGGTATGCGGACGATTGGCCCACAGTGCATc < 1:1841486/43‑1 (MQ=255) | GGCCTACTTCGGAGTTACCCATCTGACGGTCAGGCAGACCGACTTCCAGACCGGAAGCGTCGATTAGGGTATGCGGACGATTGGCCCACAGTGCATCCATTACCGGGGTTTTAG > minE/2556014‑2556127 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |