| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,558,446 | T→C | 40.0% | G211G (GGT→GGC) | gpsA → | glycerol‑3‑phosphate dehydrogenase (NAD+) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,558,446 | 0 | T | C | 40.0% | 24.6 / 44.1 | 65 | G211G (GGT→GGC) | gpsA | glycerol‑3‑phosphate dehydrogenase (NAD+) |
| Reads supporting (aligned to +/- strand): ref base T (21/18); new base C (14/12); total (35/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01 | |||||||||||
CTTGGCGGCGCGGTGAAAAACGTTATTGCCATTGGTGCGGGGATGTCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTCGCGTCTTGGTG > minE/2558381‑2558504 | cTTGGCGGCGCGGTGAAAAACGTTATTGCCATTGGTGCGGGGATGTCCGACGGTATCGGTTTTGGTGCg < 1:662853/69‑1 (MQ=255) tGGCGGCGCGGTGAAAAACGTTATTGCCATTGGTGCGGGGATGTCCGACGGTATCGGTTTTGGTGCg < 1:34887/67‑1 (MQ=255) ggTGCGGGGATGTCCGACGGTATCGGTTTTGGTGCGAATgcg < 1:745667/42‑1 (MQ=255) ggTGCGGGGATGTCCGACGGTATCGGTTTTGGTGCGAATgcg < 1:2640090/42‑1 (MQ=255) ggTGCGGGGATGTCCGACGGTATCGGTTTTGGTGCGAATgcg < 1:1408664/42‑1 (MQ=255) ggTGCGGGGATGTCCGACGGTATCGGTTTTGGTGCGAATgcg < 1:2542503/42‑1 (MQ=255) ggTGCGGGGATGTCCGACGGTATCGGTTTTGGTGCGAATgcg < 1:1674984/42‑1 (MQ=255) ggTGCGGGGATGGCCGACGGTATCGGTTTTGGTGCGAATgcg < 1:124379/42‑1 (MQ=255) gggATGTCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGa < 1:582777/68‑1 (MQ=255) gggATGTCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGa < 1:3039309/68‑1 (MQ=255) gggATGTCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGa < 1:3253003/68‑1 (MQ=255) gggATGTCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGa < 1:2488369/68‑1 (MQ=255) gggATGTCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGa < 1:456660/68‑1 (MQ=255) gggATGTCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGa < 1:465402/68‑1 (MQ=255) gggATGTCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGa < 1:1587031/68‑1 (MQ=255) gggATGTCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGa < 1:1281274/68‑1 (MQ=255) gggATGTCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGa < 1:669609/68‑1 (MQ=255) gggATGTCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGa < 1:1227560/68‑1 (MQ=255) tCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTcg > 1:2286496/1‑69 (MQ=255) tCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTcg > 1:2318988/1‑69 (MQ=255) tCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTcg > 1:973152/1‑69 (MQ=255) tCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTcg > 1:3155020/1‑69 (MQ=255) tCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTcg > 1:3304575/1‑69 (MQ=255) tCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTcg > 1:386533/1‑69 (MQ=255) tCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTcg > 1:442720/1‑69 (MQ=255) tCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTcg > 1:583065/1‑69 (MQ=255) tCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTcg > 1:1155232/1‑69 (MQ=255) tCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTcg > 1:1723033/1‑69 (MQ=255) tCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTcg > 1:1815391/1‑69 (MQ=255) tCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTcg > 1:1188448/1‑69 (MQ=255) tCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTcg > 1:1882745/1‑69 (MQ=255) tCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTcg > 1:144655/1‑69 (MQ=255) tCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTcg > 1:1424741/1‑69 (MQ=255) aCGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTCGCGTc > 1:1818865/1‑69 (MQ=255) aCGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTCGCGTc > 1:3070462/1‑69 (MQ=255) aCGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTCGCGTc > 1:1863774/1‑69 (MQ=255) aCGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTAGCGTc > 1:358289/1‑69 (MQ=255) aCGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGATGAAATGTCGCGTc > 1:1887143/1‑69 (MQ=255) aTCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTCGCGTCTTGGt > 1:2958844/1‑69 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGgctggctg > 1:2590880/1‑51 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGt > 1:1263267/1‑62 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg > 1:3243706/1‑67 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg < 1:1330267/69‑3 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg > 1:467302/1‑67 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg > 1:1678912/1‑67 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg > 1:70263/1‑67 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg < 1:1697462/69‑3 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg < 1:789875/69‑3 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg > 1:855101/1‑67 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg > 1:1764783/1‑67 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg < 1:1167557/69‑3 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg > 1:1773304/1‑67 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg < 1:1801138/69‑3 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg > 1:2233943/1‑67 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg < 1:3045790/69‑3 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg > 1:1849929/1‑67 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg < 1:301407/69‑3 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg > 1:3002511/1‑67 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg < 1:2646968/69‑3 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg < 1:2538764/69‑3 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg < 1:2528285/69‑3 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg > 1:2370741/1‑67 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg < 1:2067837/69‑3 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg > 1:2315955/1‑67 (MQ=255) tCGGTTTTGGCGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAGATGTCGCGTCTTggcg < 1:2090468/69‑3 (MQ=255) | CTTGGCGGCGCGGTGAAAAACGTTATTGCCATTGGTGCGGGGATGTCCGACGGTATCGGTTTTGGTGCGAATGCGCGTACGGCGCTGATCACCCGTGGGCTGGCTGAAATGTCGCGTCTTGGTG > minE/2558381‑2558504 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |