| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,561,301 | G→C | 31.9% | V116V (GTC→GTG) | lldD ← | L‑lactate dehydrogenase, FMN‑linked |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,561,301 | 0 | G | C | 31.9% | 77.3 / 50.5 | 72 | V116V (GTC→GTG) | lldD | L‑lactate dehydrogenase, FMN‑linked |
| Reads supporting (aligned to +/- strand): ref base G (25/24); new base C (4/19); total (29/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.44e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.58e-01 | |||||||||||
ACGCATAAAGCCGCGATCGCGCAGTACATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTAAACGGAATACCATGCGCGTCCGCCGC > minE/2561235‑2561366 | aCGCATAAAGCCGCGATCGCGCAGTACATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGAc < 1:1422024/69‑1 (MQ=255) aaGCCGCGATCGCGCAGTACATAAAGCTGGACCCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCa > 1:1061923/1‑69 (MQ=255) aaGCCGCGATCGCGCAGTACATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGActtcttaa > 1:494330/1‑67 (MQ=255) aaGCCGCGATCGCGCAGTACATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCa > 1:2875697/1‑69 (MQ=255) aaGCCGCGATCGCGCAGTACATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCa > 1:1434428/1‑69 (MQ=255) aaGCCGCGATCGCGCAGTACATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCa > 1:2159444/1‑69 (MQ=255) aaGCCGCGATCGCGCAGTACATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCa > 1:553033/1‑69 (MQ=255) aaGCCGCGATCGCGCAGTACATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCa > 1:508111/1‑69 (MQ=255) aaGCCGCGATCGCGCAGTACATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCa > 1:3131604/1‑69 (MQ=255) aaGCCGCGATCGCGCAGTACATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCa > 1:581391/1‑69 (MQ=255) aaGCCGCGATCGCGCAGTACATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCa > 1:1595059/1‑69 (MQ=255) aaGCCGCGATCGCGCAGTACATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCa > 1:1021775/1‑69 (MQ=255) cgcgCAGCACATAAAGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCAATCGGGCaaa > 1:3017741/1‑69 (MQ=255) cgcgCAGCACATAAAGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCAATCGGGCaaa > 1:1139961/1‑69 (MQ=255) cgcgCAGCACATAAAGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCAATCGGGCaaa > 1:1108988/1‑69 (MQ=255) cgcgCAGCACATAAAGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCAATCGGGCaaa > 1:2001948/1‑69 (MQ=255) aCATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAAcc > 1:1826899/1‑69 (MQ=255) aCATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAAcc > 1:2715339/1‑69 (MQ=255) aCAAAAAGCTGGAACCACATAGGGCGCTTGATGGCTGGCGCGACTACTTCa > 1:970564/1‑51 (MQ=255) cATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAAc > 1:992167/1‑67 (MQ=255) cATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCg > 1:2223223/1‑69 (MQ=255) cATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCg > 1:2084687/1‑69 (MQ=255) cATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCg > 1:1843752/1‑69 (MQ=255) cATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCg > 1:264171/1‑69 (MQ=255) cATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCg > 1:1688708/1‑69 (MQ=255) cATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCg > 1:907533/1‑69 (MQ=255) cATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCg > 1:1279038/1‑69 (MQ=255) cATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCg > 1:1307711/1‑69 (MQ=255) cATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCg > 1:1415328/1‑69 (MQ=255) cATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCg > 1:444178/1‑69 (MQ=255) tAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTc < 1:447031/69‑1 (MQ=255) aaGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCa < 1:3393577/46‑1 (MQ=255) aaGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCa < 1:3351265/46‑1 (MQ=255) aaGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCa < 1:3159494/46‑1 (MQ=255) aaGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCa < 1:3399499/46‑1 (MQ=255) aaGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCa < 1:416870/46‑1 (MQ=255) aaGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCa < 1:3048944/46‑1 (MQ=255) aaGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCa < 1:588844/46‑1 (MQ=255) aaGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCa < 1:6092/46‑1 (MQ=255) aaGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCa < 1:2837428/46‑1 (MQ=255) aaGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCa < 1:413644/46‑1 (MQ=255) aaGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCa < 1:1027620/46‑1 (MQ=255) aaGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCa < 1:1202310/46‑1 (MQ=255) aaGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCa < 1:1234405/46‑1 (MQ=255) aaGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCa < 1:125531/46‑1 (MQ=255) aaGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCa < 1:2531921/46‑1 (MQ=255) aaGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCa < 1:1621022/46‑1 (MQ=255) aaGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCa < 1:164616/46‑1 (MQ=255) aaGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCa < 1:2623845/46‑1 (MQ=255) aaGCTGGAACCACATCGGACGTTTGATAGCCGGAGCCACTTCTTCa < 1:208158/46‑1 (MQ=255) aCCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTa < 1:2135558/68‑1 (MQ=255) aCCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTa < 1:1369561/68‑1 (MQ=255) aCCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTa < 1:1643943/68‑1 (MQ=255) aCCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTa < 1:1814554/68‑1 (MQ=255) aCCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTa < 1:3330187/68‑1 (MQ=255) aCCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTa < 1:2292282/68‑1 (MQ=255) aCCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTa < 1:2325415/68‑1 (MQ=255) aCCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTa < 1:2331874/68‑1 (MQ=255) aCCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTa < 1:2921348/68‑1 (MQ=255) aCCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTa < 1:922970/68‑1 (MQ=255) aCCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTa < 1:2756610/68‑1 (MQ=255) caTTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTAAACgg < 1:214829/69‑1 (MQ=255) caTTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTAAACgg < 1:1580241/69‑1 (MQ=255) caTTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTAAACgg < 1:260521/69‑1 (MQ=255) caTTGGGAGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTAAACgg < 1:2408595/69‑1 (MQ=255) gcgcTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTAAACGGAATAc < 1:2004935/68‑1 (MQ=255) gcgcTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTAAACGGAATAc < 1:1096677/68‑1 (MQ=255) gcgcTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTAAACGGAATAc < 1:897238/68‑1 (MQ=255) gcgcTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTAAACGGAATAc < 1:1614337/68‑1 (MQ=255) gcgcTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTAAACGGAATAc < 1:2888480/68‑1 (MQ=255) gcgcTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTAAACGGAATAc < 1:2775950/68‑1 (MQ=255) gcTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTa < 1:1033305/56‑1 (MQ=255) gcgACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTAAACGGAATACCATGCGCGTCcgccgc < 1:1092494/68‑1 (MQ=255) | ACGCATAAAGCCGCGATCGCGCAGTACATAAAGCTGGAACCACATTGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTAAACGGAATACCATGCGCGTCCGCCGC > minE/2561235‑2561366 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |