| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 220,157 | A→G | 41.8% | D657D (GAT→GAC) | fadE ← | acyl coenzyme A dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 220,157 | 0 | A | G | 41.8% | 19.9 / 48.2 | 67 | D657D (GAT→GAC) | fadE | acyl coenzyme A dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (24/15); new base G (23/5); total (47/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGG > minE/220092‑220221 | gTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATcatcc < 1:3312239/69‑1 (MQ=255) gggAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATcatccatc > 1:3256516/1‑69 (MQ=255) gAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCGTCCATCg < 1:1077559/68‑1 (MQ=255) gAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCGTCCATCg < 1:1228942/68‑1 (MQ=255) gAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCGTCCATCg < 1:139056/68‑1 (MQ=255) gAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCGTCCATCg < 1:1417619/68‑1 (MQ=255) gAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCGTCCATCg < 1:2374486/68‑1 (MQ=255) aGATCACCACATTCAGCAGCCCGGCAACCCCGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCt > 1:763275/1‑69 (MQ=255) aGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCt > 1:2130932/1‑69 (MQ=255) aGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCt > 1:3308136/1‑69 (MQ=255) aGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCt > 1:2315279/1‑69 (MQ=255) aGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCt > 1:1675948/1‑69 (MQ=255) aGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCt > 1:1489706/1‑69 (MQ=255) aGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCt > 1:1299067/1‑69 (MQ=255) aTCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTg > 1:982653/1‑68 (MQ=255) aTCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTg > 1:19525/1‑68 (MQ=255) aTCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTg > 1:2223199/1‑68 (MQ=255) caccacATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAGATCATCCATCGCCTGTTc > 1:1448361/1‑69 (MQ=255) caccacATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTc > 1:882173/1‑69 (MQ=255) caccacATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTc > 1:2114466/1‑69 (MQ=255) caccacATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTc > 1:1225854/1‑69 (MQ=255) caccacATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTc > 1:3403579/1‑69 (MQ=255) caccacATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTc > 1:3054701/1‑69 (MQ=255) caccacATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTc > 1:884952/1‑69 (MQ=255) caccacATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTc > 1:188054/1‑69 (MQ=255) caccacATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTc > 1:1679423/1‑69 (MQ=255) caccacATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTc > 1:1677222/1‑69 (MQ=255) caTTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCt < 1:1463371/69‑1 (MQ=255) caTTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCt < 1:1774700/69‑1 (MQ=255) caTTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCt < 1:2556118/69‑1 (MQ=255) caTTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCt < 1:1484780/69‑1 (MQ=255) ttCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGa < 1:362587/69‑1 (MQ=255) ttCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGa < 1:2673162/69‑1 (MQ=255) ttCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGa < 1:2109125/69‑1 (MQ=255) ttCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGGTCAGCCTGa < 1:2407435/69‑1 (MQ=255) ccGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCa < 1:1557888/69‑1 (MQ=255) aaCCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTg < 1:1979500/69‑1 (MQ=255) aaCCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTg < 1:502896/69‑1 (MQ=255) aaCCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTg < 1:627586/69‑1 (MQ=255) aaCCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTg < 1:985903/69‑1 (MQ=255) aCCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTg < 1:1891999/68‑1 (MQ=255) cgcgGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGc < 1:1541038/69‑1 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATa > 1:1891564/1‑50 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATACAGCGCATCCTGTAcac > 1:1132225/1‑65 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATACAGCGCATCCTGTAcac > 1:896303/1‑65 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATACAGCGCATCCTGTAcac > 1:1880553/1‑65 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATACAGCGCATCCTGTAcac > 1:1887662/1‑65 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATACAGCGCATCCTGTAcac > 1:865182/1‑65 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATACAGCGCATCCTGTAcac > 1:1111516/1‑65 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATACAGCGCATCCTGTAcac > 1:907031/1‑65 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATACAGCGCATCCTGTAcac > 1:593447/1‑65 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATACAGCGCATCCTGTAcac > 1:191151/1‑65 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATACAGCGCATCCTGTAcac > 1:3432016/1‑65 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATACAGCGCATCCTGTAcac > 1:2009566/1‑65 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATACAGCGCATCCTGTAcac > 1:2021883/1‑65 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATACAGCGCATCCTGTAcac > 1:1059105/1‑65 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATACAGCGCATCCTGTAcac > 1:3201898/1‑65 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATACAGCGCATCCTGTAcac > 1:3055078/1‑65 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATACAGCGCATCCTGTAcac > 1:2178427/1‑65 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATACAGCGCATCCTGTAcac > 1:1611609/1‑65 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATACAGCGCATCCTGTAcac > 1:2884486/1‑65 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATACAGCGCATCCTGTAcac > 1:2881968/1‑65 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATACAGCGCATCCTGTAcac > 1:2336832/1‑65 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTGCCTGATACAGCGCATCCTGTAca > 1:2830073/1‑65 (MQ=255) cgGTTCGGGAAGTTTTGCAGTAAATCGTCCATCGCCTGTTCTCCCTGATACAGCGCATCCTGTAcac > 1:996610/1‑65 (MQ=255) aaTCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCgg > 1:1890003/1‑69 (MQ=255) aaTCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCgg > 1:1474317/1‑69 (MQ=255) aaTCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCgg > 1:3030353/1‑69 (MQ=255) aaTCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCgg > 1:2199534/1‑69 (MQ=255) | GTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAATCATCCATCGCCTGTTCAGCCTGATACAGCGCATCTTGTACGCCCCAGTGCACCAGCGGCAGGTCGG > minE/220092‑220221 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |