| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,562,254 | C→T | 41.1% | V56V (GTG→GTA) | lldR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,562,254 | 0 | C | T | 41.1% | 36.4 / 63.5 | 90 | V56V (GTG→GTA) | lldR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base C (14/39); new base T (29/8); total (43/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 | |||||||||||
TGTCATGACGCCAGCGAATAAACGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGTTGCATCGCCAGTTGGCGCTC > minE/2562193‑2562322 | tgtCATGACGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGtt < 1:2467631/69‑1 (MQ=255)tgtCATGACGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGtt < 1:1779858/69‑1 (MQ=255)tgtCATGACGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGtt < 1:1607866/69‑1 (MQ=255)tgtCATGACGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGtt < 1:34374/69‑1 (MQ=255)tgtCATGACGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGtt < 1:669461/69‑1 (MQ=255)tgtCATGACGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGtt < 1:2295830/69‑1 (MQ=255) gtCATGACGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGtt < 1:842119/68‑1 (MQ=255) tCATGACGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGtt < 1:1459519/67‑1 (MQ=255) aCGCCAGCGAATAAACGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGt < 1:1503534/61‑1 (MQ=255) aCGCCAGCGAATAAACGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAg > 1:3135761/1‑69 (MQ=255) aCGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACCCCTTCGCTTACCAGTTTCGCCAg > 1:2657803/1‑69 (MQ=255) aCGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCa > 1:2210985/1‑68 (MQ=255) aCGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCa > 1:436689/1‑68 (MQ=255) aCGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAg > 1:278270/1‑69 (MQ=255) aCGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAg > 1:1958370/1‑69 (MQ=255) aCGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAg > 1:2815374/1‑69 (MQ=255) aCGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAg > 1:3236630/1‑69 (MQ=255) aCGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAg > 1:1909993/1‑69 (MQ=255) aCGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAg > 1:1869038/1‑69 (MQ=255) aCGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAg > 1:967578/1‑69 (MQ=255) aCGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAg > 1:1684566/1‑69 (MQ=255) aCGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAg > 1:514834/1‑69 (MQ=255) aCGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAg > 1:802202/1‑69 (MQ=255) aCGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAg > 1:1109741/1‑69 (MQ=255) aCGCCAGCGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAg > 1:1592134/1‑69 (MQ=255) aCGCCAGCGAATAAACGTCCCGCCGCCAAGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAg > 1:395311/1‑69 (MQ=255) cGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAGCGCCTcg > 1:662973/1‑69 (MQ=255) cGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAGCGCCTcg > 1:2362737/1‑69 (MQ=255) cGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAGCGCCTcg > 1:2536618/1‑69 (MQ=255) cGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAGCGCCTcg > 1:1114674/1‑69 (MQ=255) cGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAGCGCCTcg > 1:1135316/1‑69 (MQ=255) cGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAGCGCCTcg > 1:1211911/1‑69 (MQ=255) cGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAGCGCCTcg > 1:1345581/1‑69 (MQ=255) cGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAGCGCCTcg > 1:17662/1‑69 (MQ=255) cGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAGCGCCTcg > 1:1931601/1‑69 (MQ=255) cGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAGCGCCTcg > 1:1591865/1‑69 (MQ=255) cGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAGCGCCTcg > 1:3342586/1‑69 (MQ=255) cGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAGCGCCTcg > 1:56595/1‑69 (MQ=255) cGAATAAACGTCCCGCCGCCACGTCGACTGAGCAGCACACCTTCGCTTACCAGTTTCGCCAGCGCCTcg > 1:480546/1‑69 (MQ=255) aaCGTCCCGCCGCCGCGTCGACTGATCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAg > 1:1012729/1‑68 (MQ=255) aaCGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAg > 1:2424141/1‑68 (MQ=255) aaCGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAg > 1:1879949/1‑68 (MQ=255) aaCGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAg > 1:1873545/1‑68 (MQ=255) aaCGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAg > 1:477900/1‑68 (MQ=255) aaCGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAg > 1:1640195/1‑68 (MQ=255) aaCGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAg > 1:784326/1‑68 (MQ=255) cGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTg < 1:1936547/68‑1 (MQ=255) cGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTg < 1:3383000/68‑1 (MQ=255) cGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTg < 1:3350025/68‑1 (MQ=255) cGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTg < 1:2194821/68‑1 (MQ=255) cGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTg < 1:852750/68‑1 (MQ=255) cGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTg < 1:3235681/68‑1 (MQ=255) cGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTg < 1:322245/68‑1 (MQ=255) cGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTg < 1:3213057/68‑1 (MQ=255) cGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTg < 1:3181433/68‑1 (MQ=255) cGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTg < 1:302000/68‑1 (MQ=255) cGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTg < 1:1603699/68‑1 (MQ=255) cGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTg < 1:2902873/68‑1 (MQ=255) cGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTg < 1:756462/68‑1 (MQ=255) cGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTg < 1:835012/68‑1 (MQ=255) gccgccgcGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACg < 1:837777/69‑1 (MQ=255) gccgccgcGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACg < 1:401970/69‑1 (MQ=255) gccgccgcGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACg < 1:993090/69‑1 (MQ=255) gccgccgcGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACg < 1:248944/69‑1 (MQ=255) gccgccgcGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACg < 1:1897085/69‑1 (MQ=255) ccgccgcGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGt < 1:44940/69‑1 (MQ=255) gccgcGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGa < 1:1005308/69‑1 (MQ=255) gccgcGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGa < 1:494467/69‑1 (MQ=255) gccgcGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGa < 1:1600278/69‑1 (MQ=255) tCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTg < 1:1935041/53‑1 (MQ=255) tGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGt > 1:898074/1‑68 (MQ=255) tGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGt > 1:257678/1‑68 (MQ=255) tGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGt > 1:358390/1‑68 (MQ=255) aCGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTTCGTGATACGCCGAGTTGCATCGcc < 1:605982/69‑1 (MQ=255) aCGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGTTGCATCGcc < 1:2818188/69‑1 (MQ=255) aCGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGTTGCATCGcc < 1:57735/69‑1 (MQ=255) aCGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGTTGCATCGcc < 1:1982562/69‑1 (MQ=255) aCGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGTTGCATCGcc < 1:1048227/69‑1 (MQ=255) aCGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGTTGCATCGcc < 1:2176598/69‑1 (MQ=255) aCGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGTTGCATCGcc < 1:2588515/69‑1 (MQ=255) aCGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGTTGCATCGcc < 1:1268446/69‑1 (MQ=255) aCGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGTTGCATCGcc < 1:1444214/69‑1 (MQ=255) aCGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGTTGCATCGcc < 1:530981/69‑1 (MQ=255) aCGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGTTGCATCGcc < 1:2910078/69‑1 (MQ=255) aCGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGTTGCATCGcc < 1:469965/69‑1 (MQ=255) aCGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGTTGCATCGcc < 1:449362/69‑1 (MQ=255) aCGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGTTGCATCGcc < 1:445156/69‑1 (MQ=255) cGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGTTGCATCGCCa > 1:1930493/1‑69 (MQ=255) cGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGTTGCATCGCCa > 1:1039537/1‑69 (MQ=255) cGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGTTGCATCGCCa > 1:1896919/1‑69 (MQ=255) cacCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGTTGCATCGCCAGTTGGCGCTc > 1:742118/1‑69 (MQ=255) cacCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGTTGCATCGCCAGTTGGCGCTc > 1:3259958/1‑69 (MQ=255) | TGTCATGACGCCAGCGAATAAACGTCCCGCCGCCGCGTCGACTGAGCAGCACGCCTTCACTCACCAGTTTTGCCAGCGCCTCGCGCAGTGAATTACGTGATACGCCGAGTTGCATCGCCAGTTGGCGCTC > minE/2562193‑2562322 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |