BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,563,066	A→G	19.9%	N337N (AAT→AAC)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,563,066	0	A	G	19.9%	167.5 / 30.2	115	N337N (AAT→AAC)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base A (66/26); new base G (15/8); total (81/34)
Fisher's exact test for biased strand distribution p-value = 6.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 116 total aligned reads displayed.

GGCTGTAGCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCAC  >  minE/2563000‑2563132

ggCTGTAGCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATAtt                                                                  >  1:330215/1‑69 (MQ=255)

ggCTGTAGCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATAtt                                                                  >  1:2860988/1‑69 (MQ=255)

ggCTGTAGCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATAtt                                                                  >  1:2864733/1‑69 (MQ=255)

ggCTGTAGCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATAtt                                                                  >  1:2866159/1‑69 (MQ=255)

ggCTGTAGCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATAtt                                                                  >  1:210997/1‑69 (MQ=255)

ggCTGTAGCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATAtt                                                                  >  1:1388577/1‑69 (MQ=255)

ggCTGTAGCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATAtt                                                                  >  1:3325678/1‑69 (MQ=255)

ggCTGTAGCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATAtt                                                                  >  1:1235194/1‑69 (MQ=255)

ggCTGTAGCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATAtt                                                                  >  1:1903939/1‑69 (MQ=255)

ggCTGTAGCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATAtt                                                                  >  1:951004/1‑69 (MQ=255)

ggCTGTAGCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATAtt                                                                  >  1:2406966/1‑69 (MQ=255)

          tCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATcacaca                                                         >  1:2974934/1‑68 (MQ=255)

          tCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCa                                                         >  1:1089034/1‑68 (MQ=255)

          tCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCa                                                         >  1:267558/1‑68 (MQ=255)

          tCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCa                                                         >  1:3008666/1‑68 (MQ=255)

              tGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCa                                                     <  1:308207/68‑1 (MQ=255)

              tGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCa                                                     <  1:2600023/68‑1 (MQ=255)

              tGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCa                                                     <  1:2562322/68‑1 (MQ=255)

              tGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCa                                                     <  1:1772994/68‑1 (MQ=255)

              tGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCa                                                     <  1:2882831/68‑1 (MQ=255)

              tGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCa                                                     <  1:314577/68‑1 (MQ=255)

              tGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCa                                                     <  1:1908982/68‑1 (MQ=255)

              tGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCa                                                     <  1:1988627/68‑1 (MQ=255)

              tGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATT‑ATCACCCACTCa                                                     <  1:2017999/67‑1 (MQ=255)

                  cACTGGCGCCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAg                                                >  1:1964685/1‑69 (MQ=255)

                    cTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATGTTGATCACCCACTCATACAgcg                                              >  1:2720411/1‑69 (MQ=255)

                    cTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATGTTGATCACCCACTCATACAgcg                                              >  1:692231/1‑69 (MQ=255)

                    cTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATGTTGATCACCCACTCATACAgcg                                              >  1:959210/1‑69 (MQ=255)

                         ggcATACGGGCAACCAGTTTATAGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGcc                                          <  1:953078/68‑1 (MQ=255)

                            aTACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGa                                                                >  1:2434079/1‑43 (MQ=255)

                            aTACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAccc                                      >  1:2290829/1‑69 (MQ=255)

                            aTACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAccc                                      >  1:2132039/1‑69 (MQ=255)

                            aTACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAccc                                      >  1:411219/1‑69 (MQ=255)

                            aTACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAccc                                      >  1:2489304/1‑69 (MQ=255)

                            aTACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAccc                                      >  1:1915118/1‑69 (MQ=255)

                            aTACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAccc                                      >  1:1064767/1‑69 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTg                                                                 >  1:1563182/1‑39 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTg                                                                 >  1:3002061/1‑39 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTg                                                                 >  1:1656164/1‑39 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTg                                                                 >  1:3089406/1‑39 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTg                                                                 >  1:1400440/1‑39 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTg                                                                 >  1:1717360/1‑39 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTg                                                                 >  1:1733959/1‑39 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTg                                                                 >  1:594981/1‑39 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTc                                                      >  1:916510/1‑50 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCa                                                     >  1:2311738/1‑51 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCa                                                     >  1:412126/1‑51 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCa                                                     >  1:1192691/1‑51 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCa                                                     >  1:1954798/1‑51 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCa                                                     >  1:1712011/1‑51 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCa                                                     >  1:3335425/1‑51 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCa                                                     >  1:3313128/1‑51 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCa                                                     >  1:1707954/1‑51 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCa                                                     >  1:3031133/1‑51 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCa                                                     >  1:1890892/1‑51 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCa                                                     >  1:270100/1‑51 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCa                                                     >  1:952754/1‑51 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCa                                                     >  1:1654277/1‑51 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGa                                    >  1:710616/1‑68 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGa                                    >  1:3198426/1‑68 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGa                                    >  1:840067/1‑68 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                   >  1:1452026/1‑69 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                   >  1:1558489/1‑69 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                   >  1:1025038/1‑69 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                   >  1:2952000/1‑69 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                   >  1:2232795/1‑69 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                   >  1:2021131/1‑69 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                   >  1:1673325/1‑69 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                   >  1:167880/1‑69 (MQ=255)

                               cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGAGCCACCCGAt                                   >  1:716463/1‑69 (MQ=255)

                                         gTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAggg                         <  1:1060562/69‑1 (MQ=255)

                                         gTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAggg                         <  1:106885/69‑1 (MQ=255)

                                         gTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAggg                         <  1:3358021/69‑1 (MQ=255)

                                         gTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAggg                         <  1:810531/69‑1 (MQ=255)

                                         gTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAggg                         <  1:2207866/69‑1 (MQ=255)

                                            tATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAc                                        >  1:1284573/1‑51 (MQ=255)

                                            tATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCt                       >  1:2647828/1‑68 (MQ=255)

                                            tATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCt                       >  1:661396/1‑68 (MQ=255)

                                               cGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTa                    <  1:3064158/68‑1 (MQ=255)

                                                       ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                >  1:1602433/1‑48 (MQ=255)

                                                       ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                >  1:1851092/1‑48 (MQ=255)

                                                       ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                >  1:2211077/1‑48 (MQ=255)

                                                       ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                >  1:2019221/1‑48 (MQ=255)

                                                       ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                >  1:533819/1‑48 (MQ=255)

                                                       ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                >  1:3434303/1‑48 (MQ=255)

                                                       ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                >  1:3407137/1‑48 (MQ=255)

                                                       ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                >  1:1254310/1‑48 (MQ=255)

                                                       ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                >  1:255220/1‑48 (MQ=255)

                                                       ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                >  1:3037594/1‑48 (MQ=255)

                                                       ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                >  1:162437/1‑48 (MQ=255)

                                                          aCCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATa        <  1:1014244/69‑1 (MQ=255)

                                                           ccGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc       <  1:2336365/69‑1 (MQ=255)

                                                           ccGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc       <  1:335611/69‑1 (MQ=255)

                                                           ccGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc       <  1:724279/69‑1 (MQ=255)

                                                           ccGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc       <  1:3268246/69‑1 (MQ=255)

                                                           ccGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc       <  1:1642965/69‑1 (MQ=255)

                                                            cGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc       <  1:505784/68‑1 (MQ=255)

                                                            cGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc       <  1:874831/68‑1 (MQ=255)

                                                             ggAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc       <  1:2365039/67‑1 (MQ=255)

                                                                atatTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCcac  <  1:2104740/69‑1 (MQ=255)

GGCTGTAGCCTCGCTGACCACTGGCGGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCAC  >  minE/2563000‑2563132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`