BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,563,090	G→A	18.2%	G329G (GGC→GGT)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,563,090	0	G	A	18.2%	174.3 / 23.7	109	G329G (GGC→GGT)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base G (75/14); new base A (12/8); total (87/22)
Fisher's exact test for biased strand distribution p-value = 2.71e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 114 total aligned reads displayed.

GGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTCAGG  >  minE/2563025‑2563153

   aTACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAccc                                                           >  1:411219/1‑69 (MQ=255)

   aTACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAccc                                                           >  1:1064767/1‑69 (MQ=255)

   aTACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAccc                                                           >  1:2290829/1‑69 (MQ=255)

   aTACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAccc                                                           >  1:1915118/1‑69 (MQ=255)

   aTACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAccc                                                           >  1:2132039/1‑69 (MQ=255)

   aTACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAcc                                                            >  1:1222610/1‑68 (MQ=255)

      cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGa                                                         >  1:3198426/1‑68 (MQ=255)

      cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGa                                                         >  1:840067/1‑68 (MQ=255)

      cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGa                                                         >  1:710616/1‑68 (MQ=255)

      cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                        >  1:2952000/1‑69 (MQ=255)

      cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                        >  1:1673325/1‑69 (MQ=255)

      cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                        >  1:167880/1‑69 (MQ=255)

      cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                        >  1:1558489/1‑69 (MQ=255)

      cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                        >  1:364046/1‑69 (MQ=255)

      cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                        >  1:1452026/1‑69 (MQ=255)

      cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                        >  1:2232795/1‑69 (MQ=255)

      cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                        >  1:1025038/1‑69 (MQ=255)

      cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGAGCCACCCGAt                                                        >  1:716463/1‑69 (MQ=255)

                gTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAggg                                              <  1:2207866/69‑1 (MQ=255)

                gTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAggg                                              <  1:810531/69‑1 (MQ=255)

                gTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAggg                                              <  1:106885/69‑1 (MQ=255)

                gTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAggg                                              <  1:1060562/69‑1 (MQ=255)

                 tttATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAggg                                              <  1:2037456/68‑1 (MQ=255)

                   tATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAc                                                             >  1:1284573/1‑51 (MQ=255)

                   tATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCt                                            >  1:2647828/1‑68 (MQ=255)

                   tATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCt                                            >  1:661396/1‑68 (MQ=255)

                      cGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTa                                         <  1:3064158/68‑1 (MQ=255)

                              ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                     >  1:2211077/1‑48 (MQ=255)

                              ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                     >  1:1254310/1‑48 (MQ=255)

                              ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                     >  1:162437/1‑48 (MQ=255)

                              ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                     >  1:3037594/1‑48 (MQ=255)

                              ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                     >  1:2019221/1‑48 (MQ=255)

                              ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                     >  1:2711981/1‑48 (MQ=255)

                              ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                     >  1:3407137/1‑48 (MQ=255)

                              ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                     >  1:3434303/1‑48 (MQ=255)

                              ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                     >  1:533819/1‑48 (MQ=255)

                              ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                     >  1:1851092/1‑48 (MQ=255)

                              ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                     >  1:255220/1‑48 (MQ=255)

                              ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                     >  1:1602433/1‑48 (MQ=255)

                                 aCCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATa                             <  1:1014244/69‑1 (MQ=255)

                                  ccGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                            <  1:3268246/69‑1 (MQ=255)

                                  ccGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                            <  1:2336365/69‑1 (MQ=255)

                                  ccGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                            <  1:1642965/69‑1 (MQ=255)

                                  ccGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                            <  1:335611/69‑1 (MQ=255)

                                  ccGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                            <  1:724279/69‑1 (MQ=255)

                                   cGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                            <  1:874831/68‑1 (MQ=255)

                                   cGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                            <  1:505784/68‑1 (MQ=255)

                                    ggAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                            <  1:2365039/67‑1 (MQ=255)

                                       atatTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCcac                       <  1:2104740/69‑1 (MQ=255)

                                          ttGATCACCCACTCATACAGCGCGCCCCCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                    >  1:2186879/1‑69 (MQ=255)

                                          ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                               >  1:879687/1‑58 (MQ=255)

                                          ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                               >  1:83235/1‑58 (MQ=255)

                                          ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                               >  1:3405125/1‑58 (MQ=255)

                                          ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                               >  1:523004/1‑58 (MQ=255)

                                          ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                               >  1:2499609/1‑58 (MQ=255)

                                          ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                               >  1:1552053/1‑58 (MQ=255)

                                          ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                               >  1:2576799/1‑58 (MQ=255)

                                          ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                               >  1:1423142/1‑58 (MQ=255)

                                          ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                    >  1:1807044/1‑69 (MQ=255)

                                          ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                    >  1:1067849/1‑69 (MQ=255)

                                          ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                    >  1:2790289/1‑69 (MQ=255)

                                          ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                    >  1:1427079/1‑69 (MQ=255)

                                          ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                    >  1:2404386/1‑69 (MQ=255)

                                          ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                    >  1:756787/1‑69 (MQ=255)

                                            gATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgtgt                  <  1:2521857/69‑1 (MQ=255)

                                            gATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgtgt                  <  1:3225388/69‑1 (MQ=255)

                                             aTCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgtgt                  <  1:1595665/68‑1 (MQ=255)

                                               cACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTAc               >  1:95259/1‑69 (MQ=255)

                                               cACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTAc               >  1:909719/1‑69 (MQ=255)

                                                       cATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCcac                       >  1:2682432/1‑53 (MQ=255)

                                                       cATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCt        >  1:3403138/1‑68 (MQ=255)

                                                       cATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCt        >  1:1964187/1‑68 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCca                        >  1:3382929/1‑48 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGtaa    >  1:2329124/1‑66 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca    >  1:1227547/1‑68 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca    >  1:1196119/1‑68 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca    >  1:1300223/1‑68 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca    >  1:1358855/1‑68 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca    >  1:861595/1‑68 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca    >  1:1025549/1‑68 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca    >  1:1515714/1‑68 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca    >  1:1933842/1‑68 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca    >  1:512825/1‑68 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca    >  1:511790/1‑68 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca    >  1:3402766/1‑68 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca    >  1:2648645/1‑68 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca    >  1:252659/1‑68 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca    >  1:3357242/1‑68 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca    >  1:2170683/1‑68 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca    >  1:2751085/1‑68 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca    >  1:3296075/1‑68 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca    >  1:226752/1‑68 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca    >  1:1006855/1‑68 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTc     >  1:1929723/1‑67 (MQ=255)

                                                           cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTC‑‑CAGTGTTACGGTAGCTGTcagg  >  1:1610651/1‑68 (MQ=255)

                                                              cgcgcCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTcagg  >  1:118259/1‑67 (MQ=255)

                                                              cgcgcCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTcagg  >  1:3357366/1‑67 (MQ=255)

                                                              cgcgcCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTcagg  >  1:2658239/1‑67 (MQ=255)

                                                              cgcgcCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTcagg  >  1:339596/1‑67 (MQ=255)

                                                              cgcacCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                            <  1:794973/41‑1 (MQ=25)

GGCATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTCAGG  >  minE/2563025‑2563153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`