BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,563,093	A→G	18.3%	G328G (GGT→GGC)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,563,093	0	A	G	18.3%	169.7 / 26.7	115	G328G (GGT→GGC)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base A (78/15); new base G (12/9); total (91/24)
Fisher's exact test for biased strand distribution p-value = 1.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.83e-01

Only 100 of 115 total aligned reads displayed.

ATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTCAGGAACAGG  >  minE/2563028‑2563159

aTACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAccc                                                                 >  1:2132039/1‑69 (MQ=255)

aTACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAccc                                                                 >  1:1915118/1‑69 (MQ=255)

aTACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAccc                                                                 >  1:2290829/1‑69 (MQ=255)

aTACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAccc                                                                 >  1:2489304/1‑69 (MQ=255)

aTACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAccc                                                                 >  1:1064767/1‑69 (MQ=255)

aTACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAccc                                                                 >  1:411219/1‑69 (MQ=255)

aTACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAcc                                                                  >  1:1222610/1‑68 (MQ=255)

   cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGa                                                               >  1:840067/1‑68 (MQ=255)

   cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGa                                                               >  1:3198426/1‑68 (MQ=255)

   cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGa                                                               >  1:710616/1‑68 (MQ=255)

   cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                              >  1:1558489/1‑69 (MQ=255)

   cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                              >  1:1025038/1‑69 (MQ=255)

   cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                              >  1:167880/1‑69 (MQ=255)

   cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                              >  1:1673325/1‑69 (MQ=255)

   cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                              >  1:2232795/1‑69 (MQ=255)

   cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                              >  1:2952000/1‑69 (MQ=255)

   cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                              >  1:1452026/1‑69 (MQ=255)

   cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                              >  1:2021131/1‑69 (MQ=255)

   cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGAGCCACCCGAt                                                              >  1:716463/1‑69 (MQ=255)

             gTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAggg                                                    <  1:3358021/69‑1 (MQ=255)

             gTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAggg                                                    <  1:2207866/69‑1 (MQ=255)

             gTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAggg                                                    <  1:1060562/69‑1 (MQ=255)

             gTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAggg                                                    <  1:810531/69‑1 (MQ=255)

              tttATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAggg                                                    <  1:2037456/68‑1 (MQ=255)

                tATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCAc                                                                   >  1:1284573/1‑51 (MQ=255)

                tATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCt                                                  >  1:2647828/1‑68 (MQ=255)

                tATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCt                                                  >  1:661396/1‑68 (MQ=255)

                   cGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTa                                               <  1:3068066/68‑1 (MQ=255)

                   cGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTa                                               <  1:3064158/68‑1 (MQ=255)

                           ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:1602433/1‑48 (MQ=255)

                           ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:2019221/1‑48 (MQ=255)

                           ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:2211077/1‑48 (MQ=255)

                           ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:1851092/1‑48 (MQ=255)

                           ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:162437/1‑48 (MQ=255)

                           ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:533819/1‑48 (MQ=255)

                           ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:3037594/1‑48 (MQ=255)

                           ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:3407137/1‑48 (MQ=255)

                           ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:3434303/1‑48 (MQ=255)

                           ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:1254310/1‑48 (MQ=255)

                              aCCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATa                                   <  1:1014244/69‑1 (MQ=255)

                               ccGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                                  <  1:335611/69‑1 (MQ=255)

                               ccGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                                  <  1:3268246/69‑1 (MQ=255)

                               ccGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                                  <  1:724279/69‑1 (MQ=255)

                               ccGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                                  <  1:2336365/69‑1 (MQ=255)

                                cGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                                  <  1:505784/68‑1 (MQ=255)

                                cGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                                  <  1:874831/68‑1 (MQ=255)

                                 ggAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                                  <  1:2365039/67‑1 (MQ=255)

                                    atatTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCcac                             <  1:2104740/69‑1 (MQ=255)

                                       ttGATCACCCACTCATACAGCGCGCCCCCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                          >  1:2186879/1‑69 (MQ=255)

                                       ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCggg                                      >  1:133481/1‑57 (MQ=255)

                                       ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                                     >  1:1793253/1‑58 (MQ=255)

                                       ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                                     >  1:2499609/1‑58 (MQ=255)

                                       ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                                     >  1:83235/1‑58 (MQ=255)

                                       ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                                     >  1:2576799/1‑58 (MQ=255)

                                       ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                                     >  1:1423142/1‑58 (MQ=255)

                                       ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                                     >  1:879687/1‑58 (MQ=255)

                                       ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                                     >  1:3405125/1‑58 (MQ=255)

                                       ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                                     >  1:1114746/1‑58 (MQ=255)

                                       ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                                     >  1:523004/1‑58 (MQ=255)

                                       ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                                     >  1:2222967/1‑58 (MQ=255)

                                       ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                          >  1:2404386/1‑69 (MQ=255)

                                       ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                          >  1:1427079/1‑69 (MQ=255)

                                       ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                          >  1:2790289/1‑69 (MQ=255)

                                       ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                          >  1:1067849/1‑69 (MQ=255)

                                       ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                          >  1:756787/1‑69 (MQ=255)

                                       ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                          >  1:1807044/1‑69 (MQ=255)

                                         gATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgtgt                        <  1:2521857/69‑1 (MQ=255)

                                          aTCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgtgt                        <  1:1595665/68‑1 (MQ=255)

                                            cACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTAc                     >  1:909719/1‑69 (MQ=255)

                                                    cATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCcac                             >  1:2682432/1‑53 (MQ=255)

                                                    cATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCt              >  1:1964187/1‑68 (MQ=255)

                                                    cATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCt              >  1:3403138/1‑68 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCca                              >  1:3382929/1‑48 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:3296075/1‑68 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:1515714/1‑68 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:226752/1‑68 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:861595/1‑68 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:1006855/1‑68 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:252659/1‑68 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:1025549/1‑68 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:2648645/1‑68 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:2661648/1‑68 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:571/1‑68 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:1196119/1‑68 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:3357242/1‑68 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:512825/1‑68 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:511790/1‑68 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:2751085/1‑68 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:1227547/1‑68 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:362360/1‑68 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:1358855/1‑68 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:1569159/1‑68 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:3402766/1‑68 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTc           >  1:1929723/1‑67 (MQ=255)

                                                        cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTC‑‑CAGTGTTACGGTAGCTGTcagg        >  1:1610651/1‑68 (MQ=255)

                                                           cgcgcCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTcagg        >  1:118259/1‑67 (MQ=255)

                                                           cgcgcCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTcagg        >  1:2658239/1‑67 (MQ=255)

                                                           cgcgcCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTcagg        >  1:3357366/1‑67 (MQ=255)

                                                           cgcacCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                                  <  1:794973/41‑1 (MQ=25)

                                                               ccACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTcaggaacagg  <  1:1589783/69‑1 (MQ=255)

ATACGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTCAGGAACAGG  >  minE/2563028‑2563159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`