BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,563,099	T→A	22.3%	A326A (GCA→GCT)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,563,099	0	T	A	22.3%	163.0 / 39.7	94	A326A (GCA→GCT)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base T (58/15); new base A (12/9); total (70/24)
Fisher's exact test for biased strand distribution p-value = 4.96e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

CGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTCAGGAACAGG  >  minE/2563031‑2563159

cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                              >  1:2232795/1‑69 (MQ=255)

cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                              >  1:1673325/1‑69 (MQ=255)

cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                              >  1:167880/1‑69 (MQ=255)

cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                              >  1:364046/1‑69 (MQ=255)

cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                              >  1:2021131/1‑69 (MQ=255)

cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                              >  1:1558489/1‑69 (MQ=255)

cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                              >  1:1452026/1‑69 (MQ=255)

cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                              >  1:2952000/1‑69 (MQ=255)

cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGAt                                                              >  1:1025038/1‑69 (MQ=255)

cGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGAGCCACCCGAt                                                              >  1:716463/1‑69 (MQ=255)

          gTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAggg                                                    <  1:106885/69‑1 (MQ=255)

          gTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAggg                                                    <  1:1060562/69‑1 (MQ=255)

          gTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAggg                                                    <  1:2207866/69‑1 (MQ=255)

          gTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAggg                                                    <  1:810531/69‑1 (MQ=255)

          gTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAggg                                                    <  1:3358021/69‑1 (MQ=255)

           tttATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAggg                                                    <  1:2037456/68‑1 (MQ=255)

             tATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCt                                                  >  1:661396/1‑68 (MQ=255)

             tATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCt                                                  >  1:2647828/1‑68 (MQ=255)

                cGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTa                                               <  1:3064158/68‑1 (MQ=255)

                cGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTa                                               <  1:3068066/68‑1 (MQ=255)

                        ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:3407137/1‑48 (MQ=255)

                        ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:1851092/1‑48 (MQ=255)

                        ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:2211077/1‑48 (MQ=255)

                        ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:3434303/1‑48 (MQ=255)

                        ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:2711981/1‑48 (MQ=255)

                        ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:255220/1‑48 (MQ=255)

                        ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:2019221/1‑48 (MQ=255)

                        ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:3037594/1‑48 (MQ=255)

                        ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:162437/1‑48 (MQ=255)

                        ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:1602433/1‑48 (MQ=255)

                        ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:533819/1‑48 (MQ=255)

                        ggCACCGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCg                                                           >  1:1254310/1‑48 (MQ=255)

                           aCCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATa                                   <  1:1014244/69‑1 (MQ=255)

                            ccGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                                  <  1:3268246/69‑1 (MQ=255)

                            ccGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                                  <  1:335611/69‑1 (MQ=255)

                            ccGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                                  <  1:724279/69‑1 (MQ=255)

                            ccGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                                  <  1:2336365/69‑1 (MQ=255)

                            ccGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                                  <  1:1642965/69‑1 (MQ=255)

                             cGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                                  <  1:874831/68‑1 (MQ=255)

                             cGGAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                                  <  1:505784/68‑1 (MQ=255)

                              ggAATGTTGATCACCCACTCATACAGCGCACCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                                  <  1:2365039/67‑1 (MQ=255)

                                 atatTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCcac                             <  1:2104740/69‑1 (MQ=255)

                                    ttGATCACCCACTCATACAGCGCGCCCCCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                          >  1:2186879/1‑69 (MQ=255)

                                    ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCggg                                      >  1:133481/1‑57 (MQ=255)

                                    ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                                     >  1:2576799/1‑58 (MQ=255)

                                    ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                                     >  1:1793253/1‑58 (MQ=255)

                                    ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                                     >  1:879687/1‑58 (MQ=255)

                                    ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                                     >  1:1114746/1‑58 (MQ=255)

                                    ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                                     >  1:83235/1‑58 (MQ=255)

                                    ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                                     >  1:523004/1‑58 (MQ=255)

                                    ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                                     >  1:1552053/1‑58 (MQ=255)

                                    ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                                     >  1:1423142/1‑58 (MQ=255)

                                    ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                                     >  1:3405125/1‑58 (MQ=255)

                                    ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGa                                     >  1:2222967/1‑58 (MQ=255)

                                    ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                          >  1:2404386/1‑69 (MQ=255)

                                    ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                          >  1:824308/1‑69 (MQ=255)

                                    ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                          >  1:756787/1‑69 (MQ=255)

                                    ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                          >  1:1427079/1‑69 (MQ=255)

                                    ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                          >  1:1807044/1‑69 (MQ=255)

                                    ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                          >  1:1067849/1‑69 (MQ=255)

                                    ttGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgt                          >  1:2790289/1‑69 (MQ=255)

                                      gATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgtgt                        <  1:2521857/69‑1 (MQ=255)

                                      gATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgtgt                        <  1:3225388/69‑1 (MQ=255)

                                       aTCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAgtgt                        <  1:1595665/68‑1 (MQ=255)

                                         cACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTAc                     >  1:909719/1‑69 (MQ=255)

                                                 cATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCcac                             >  1:2682432/1‑53 (MQ=255)

                                                 cATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCt              >  1:3403138/1‑68 (MQ=255)

                                                 cATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCt              >  1:1964187/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCca                              >  1:3382929/1‑48 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGtaa          >  1:2329124/1‑66 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:512825/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:2751085/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:1025549/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:861595/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:1196119/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:1227547/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:1300223/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:1358855/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:1515714/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:1569159/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:571/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:2661648/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:2648645/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:1933842/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:362360/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:3296075/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:2170683/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:226752/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:511790/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:252659/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:1006855/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTca          >  1:3357242/1‑68 (MQ=255)

                                                     cAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTc           >  1:1929723/1‑67 (MQ=255)

                                                        cgcgcCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTcagg        >  1:339596/1‑67 (MQ=255)

                                                        cgcgcCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTcagg        >  1:118259/1‑67 (MQ=255)

                                                        cgcgcCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTcagg        >  1:3357366/1‑67 (MQ=255)

                                                        cgcgcCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTcagg        >  1:2658239/1‑67 (MQ=255)

                                                        cgcacCGCCTGAAGCGAACAGGGCTTTAAACGGCGGGATAc                                  <  1:794973/41‑1 (MQ=25)

                                                            ccACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTcaggaacagg  <  1:1589783/69‑1 (MQ=255)

                                                             cACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTcaggaacagg  <  1:655947/68‑1 (MQ=255)

CGGGCAACCAGTTTATCGAGGTACGGCACCGGAATATTGATCACCCACTCATACAGCGCGCCACCCGATGCGAACAGGGCTTTAAACGGCGGGATACTCCACAGTGTTACGGTAGCTGTCAGGAACAGG  >  minE/2563031‑2563159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`