Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,563,240 A→G 53.2% D279D (GAT→GAC lldP ← L‑lactate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,563,2400AG53.2% 6.0 / 52.2 64D279D (GAT→GAClldPL‑lactate permease
Reads supporting (aligned to +/- strand):  ref base A (18/12);  new base G (17/17);  total (35/29)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

AACACCTGACCCGCAGTGTAACCGGTGTGGGCCAGCGTCATATCAACCTGTGACGCCCCCAAATCACCAAAACGGAATACACGCACTGGCTGCCAGCGTTTGAGGAACAGCGTCAGGCAGAGCAGTGAT  >  minE/2563178‑2563306
                                                              |                                                                  
agcacCTGACCTGCCGTGTAACCTGTGTGAGCCAGCGTCATATCAACCTGTGACGCCCCTAAGTCAccg                                                              <  1:97219/67‑2 (MQ=255)
agcacCTGACCTGCCGTGTAACCTGTGTGAGCCAGCGTCATATCAACCTGTGACGCCCCTAAGTCAccg                                                              <  1:334358/67‑2 (MQ=255)
agcacCTGACCTGCCGTGTAACCTGTGTGAGCCAGCGTCATATCAACCTGTGACGCCCCTAAGTCAccg                                                              <  1:2455995/67‑2 (MQ=255)
agcacCTGACCTGCCGTGTAACCTGTGTGAGCCAGCGTCATATCAACCTGTGACGCCCCTAAGTCAccg                                                              <  1:1647043/67‑2 (MQ=255)
 gcacCTGACCTGCCGTGTAACCTGTGTGAGCCAGCGTCATATCAACCTGTGACGCCCCTAAGTCAccg                                                              <  1:810340/67‑2 (MQ=255)
 gcacCTGACCTGCCGTGTAACCTGTGTGAGCCAGCGTCATATCAACCTGTGACGCCCCTAAGTCAccg                                                              <  1:1656580/67‑2 (MQ=255)
 acacCTGACCCGCAGTGTAACCGGTGTGGGCCAGCGTCATATCAACCTGTGACGCCCCCAAATCACCaa                                                             >  1:696899/1‑69 (MQ=255)
      tGACCCGCAGTGTAACCGGTGTGGGCCAGCGTCATATCAACCTGTGACGCCCCCAAATCACCAAAACgg                                                        <  1:591845/69‑1 (MQ=255)
      tGACCCGCAGTGTAACCGGTGTGGGCCAGCGTCATATCAACCTGTGACGCCCCCAAATCACCAAAACgg                                                        <  1:1404918/69‑1 (MQ=255)
      tGACCCGCAGTGTAACCGGTGTGGGCCAGCGTCATATCAACCTGTGACGCCCCCAAATCACCAAAACgg                                                        <  1:2182380/69‑1 (MQ=255)
          ccGCAGTGTAACCGGTGTGGGCCAGCGTCATATCAACCTGTGACGCCCCCAAATCACCAAAACGGaa                                                      <  1:1024073/67‑1 (MQ=255)
          ccGCAGTGTAACCGGTGTGGGCCAGCGTCATATCAACCTGTGACGCCCCCAAATCACCAAAACGGaa                                                      <  1:799091/67‑1 (MQ=255)
          ccGCAGTGTAACCGGTGTGGGCCAGCGTCATATCAACCTGTGACGCCCCCAAATCACCAAAACGGaa                                                      <  1:480476/67‑1 (MQ=255)
          ccGCAGTGTAACCGGTGTGGGCCAGCGTCATATCAACCTGTGACGCCCCCAAATCACCAAAACGGaa                                                      <  1:3333508/67‑1 (MQ=255)
          ccGCAGTGTAACCGGTGTGGGCCAGCGTCATATCAACCTGTGACGCCCCCAAATCACCAAAACGGaa                                                      <  1:2901773/67‑1 (MQ=255)
          ccGCAGTGTAACCGGTGTGGGCCAGCGTCATATCAACCTGTGACGCCCCAAAATCACCAAAACGGaa                                                      <  1:155850/67‑1 (MQ=255)
              aGTGTAACCGGTGTGGGCCAGCGTCATATCAACCTGTGACGCCCCCAAATCACCAAAACGGAATACACg                                                <  1:1594524/69‑1 (MQ=255)
              aGTGTAACCGGTGTGGGCCAGCGTCATATCAACCTGTGACGCCCCCAAATCACCAAAACGGAATACACg                                                <  1:1751823/69‑1 (MQ=255)
                  tAACCGGTGTGGGCCAGCGTCATATCAACCTGTGACGCCCCCAAATCACCAAAACGGAATACACGCACt                                            >  1:2390762/1‑69 (MQ=255)
                  tAACCGGTGTGGGCCAGCGTCATATCAACCTGTGACGCCCCCAAATCACCAAAACGGAATACACGCACt                                            >  1:2670049/1‑69 (MQ=255)
                  tAACCGGTGTGGGCCAGCGTCATATCAACCTGTGACGCCCCCAAATCACCAAAACGGAATACACGCACt                                            >  1:1678735/1‑69 (MQ=255)
                  tAACCGGTGTGGGCCAGCGTCATATCAACCTGTGACGCCCCCAAATCACCAAAACGGAATACACGCACt                                            >  1:3168196/1‑69 (MQ=255)
                  tAACCGGTGTGGGCCAGCGTCATATCAACCTGTGACGCCCCCAAATCACCAAAACGGAATACACGCACt                                            >  1:2679508/1‑69 (MQ=255)
                  tAACCGGTGTGGGCCAGCGTCATATCAACCTGTGACGCCCCCAAATCACCAAAACGGAATACACGCACt                                            >  1:293282/1‑69 (MQ=255)
                  tAACCGGTGTGGGCCAGCGTCATATCAACCTGTGACGCCACCAAATCACCAAAACGGAATACACGCACt                                            >  1:1537212/1‑69 (MQ=255)
                         tgtgGGCCAGCGTCATATCAACCTGTGACGCCCCCAAATCACCAAAACGGAATACACGCACTGGCTGcc                                     >  1:768093/1‑69 (MQ=255)
                         tgtgGGCCAGCGTCATATCAACCTGTGACGCCCCCAAATCACCAAAACGGAATACACGCACTGGCTGc                                      >  1:2007057/1‑68 (MQ=255)
                         tgtgAGCCAGCGTCATATCAACCTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTgg                                          <  1:1492246/64‑1 (MQ=255)
                         tgtgAGCCAGCGTCATATCAACCTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTgg                                          <  1:1147497/64‑1 (MQ=255)
                         tgtgAGCCAGCGTCATATCAACCTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTgg                                          <  1:803413/64‑1 (MQ=255)
                         tgtgAGCCAGCGTCATATCAACCTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTgg                                          <  1:3368507/64‑1 (MQ=255)
                         tgtgAGCCAGCGTCATATCAACCTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTgg                                          <  1:640991/64‑1 (MQ=255)
                         tgtgAGCCAGCGTCATATCAACCTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTgg                                          <  1:1016775/64‑1 (MQ=255)
                         tgtgAGCCAGCGTCATATCAACCTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTgg                                          <  1:181453/64‑1 (MQ=255)
                         tgtgAGCCAGCGTCATATCAACCTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTgg                                          <  1:1522772/64‑1 (MQ=255)
                         tgtgAGCCAGCGTCATATCAACCTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTgg                                          <  1:96077/64‑1 (MQ=255)
                         tgtgAGCCAGCGTCATATCAACCTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTgg                                          <  1:165382/64‑1 (MQ=255)
                          gtgAGCCAGCGTCATATCAACCTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTgg                                          <  1:2084432/63‑1 (MQ=255)
                                    gTCATATCAACCTGTGACGCCCCCAAATCACCAAAACGGAATACACGCACTGGCTGCCAGCGTTTGa                            >  1:657541/1‑67 (MQ=255)
                                    gTCATATCAACCTGTGACGCCCCCAAATCACCAAAACGGAATACACGCACTGGCTGCCAGCGTTTGAgg                          <  1:2671139/69‑1 (MQ=255)
                                    gTCATATCAACCTGTGACGCCCCCAAATCACCAAAACGGAATACACGCACTGGCTGCCAGCGTTTGAgg                          >  1:2922130/1‑69 (MQ=255)
                                          tCAACCTGTGACGCCCCCAAATCACCAAAACGGAATACACGCACTGGCTGCCAGCGTTTGAGGAACAg                     >  1:385708/1‑68 (MQ=255)
                                          tCAACCTGTGACGCCCCCAAATCACCAAAACGGAATACACGCACTGGCTGCCAGCGTTTGAGGAACAGc                    >  1:1304369/1‑69 (MQ=255)
                                          tCAACCTGTGACGCCCCCAAATCACCAAAACGGAATACACGCACTGGCTGCCAGCGTTTGAGGAACAGc                    >  1:1240080/1‑69 (MQ=255)
                                            aaCCTGTGACGCCCCCAAATCACCAAAACGGAATACACGCACTGGCTGCCAGCGTTTGAGGAACAGc                    >  1:2264526/1‑67 (MQ=255)
                                            aaCCTGTGACGCCCCCAAATCACCAAAACGGAATACACGCACTGGCTGCCAGCGTTTGAGGAACAGc                    >  1:1932790/1‑67 (MQ=255)
                                                    aCGCCCCCAAATCACCAAAACGGAATACACGCACTGGCTGCCAGCGTTTGAGGAACAGCGTCAGGCag           >  1:2265145/1‑68 (MQ=255)
                                                        ccctAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACAGTGTCAGACAGAGCa       >  1:754379/1‑68 (MQ=255)
                                                        ccctAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACAGTGTCAGACAGAGCa       >  1:731090/1‑68 (MQ=255)
                                                        ccctAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACAGTGTCAGACAGAGCa       >  1:750828/1‑68 (MQ=255)
                                                        ccctAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACAGTGTCAGACAGAGCa       >  1:1144460/1‑68 (MQ=255)
                                                        ccctAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACAGTGTCAGACAGAGCa       >  1:297779/1‑68 (MQ=255)
                                                        ccctAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACAGTGTCAGACAGAGCa       >  1:511429/1‑68 (MQ=255)
                                                        ccctAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACAGTGTCAGACAGAGCa       >  1:1715631/1‑68 (MQ=255)
                                                        ccctAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACAGTGTCAGACAGAGCa       >  1:496878/1‑68 (MQ=255)
                                                        ccctAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACAGTGTCAGACAGAGCa       >  1:3354651/1‑68 (MQ=255)
                                                        ccctAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACAGTGTCAGACAGAGCa       >  1:131391/1‑68 (MQ=255)
                                                        ccctAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACAGTGTCAGACAGAGCa       >  1:137612/1‑68 (MQ=255)
                                                        ccctAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACAGTGTCAGACAGAGCa       >  1:2995817/1‑68 (MQ=255)
                                                        ccctAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACAGTGTCAGACAGAGCa       >  1:1599262/1‑68 (MQ=255)
                                                        ccctAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACAGTGTCAGACAGAGCa       >  1:295479/1‑68 (MQ=255)
                                                        ccctAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACAGTGTCAGACAGAGCa       >  1:2875769/1‑68 (MQ=255)
                                                        ccctAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACAGTGTCAGACAGAGCa       >  1:2595515/1‑68 (MQ=255)
                                                        ccctAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACAGTGTCAGACAGAGCa       >  1:2132249/1‑68 (MQ=255)
                                                            aaaTCACCAAAACGGAATACACGCACTGGCTGCCAGCGTTTGAGGAACAGCGTCAGGCAGAGCAGTGAt  <  1:1635502/69‑1 (MQ=255)
                                                            aaaTCACCAAAACGGAATACACGCACTGGCTGCCAGCGTTTGAGGAACAGCGTCAGGCAGAGCAGTGAt  <  1:855016/69‑1 (MQ=255)
                                                            aaaTCACCAAAACGGAATACACGCACTGGCTGCCAGCGTTTGAGGAACAGCGTCAGGCAGAGCAGTGAt  <  1:630068/69‑1 (MQ=255)
                                                            aaaTCACCAAAACGGAATACACGCACTGGCTGCCAGCGTTTGAGGAACAGCGTCAGGCAGAGCAGTGAt  <  1:1260014/69‑1 (MQ=255)
                                                            aaaTCACCAAAACGGAATACACGCACTGGCTGCCAGCGTTTGAGGAACAGCGTCAGGCAGAGCAGTGAt  <  1:567478/69‑1 (MQ=255)
                                                              |                                                                  
AACACCTGACCCGCAGTGTAACCGGTGTGGGCCAGCGTCATATCAACCTGTGACGCCCCCAAATCACCAAAACGGAATACACGCACTGGCTGCCAGCGTTTGAGGAACAGCGTCAGGCAGAGCAGTGAT  >  minE/2563178‑2563306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: