| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,569,401 | C→T | 27.2% | intergenic (‑207/‑330) | mtlA ← / → yibI | fused mannitol‑specific PTS enzyme IIABC components/predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,569,401 | 0 | C | T | 27.2% | 111.1 / 37.1 | 95 | intergenic (‑207/‑330) | mtlA/yibI | fused mannitol‑specific PTS enzyme IIABC components/predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (44/25); new base T (14/12); total (58/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01 | |||||||||||
TGTGATATCAATCACATAAATATAGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGCATTTAAATGTGAGTAG > minE/2569338‑2569463 | tgtgATATCAGTCACATAAATGTAGGGGGTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCtt < 1:2134542/68‑1 (MQ=255)tgtgATATCAGTCACATAAATGTAGGGGGTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCtt < 1:108492/68‑1 (MQ=255)tgtgATATCAGTCACATAAATGTAGGGGGTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCtt < 1:227148/68‑1 (MQ=255)tgtgATATCAGTCACATAAATGTAGGGGGTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCtt < 1:2707026/68‑1 (MQ=255)tgtgATATCAGTCACATAAATGTAGGGGGTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCtt < 1:2990831/68‑1 (MQ=255)tgtgATATCAGTCACATAAATGTAGGGGGTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCtt < 1:627967/68‑1 (MQ=255)tgtgATATCAGTCACATAAATGTAGGGGGTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCtt < 1:1225041/68‑1 (MQ=255)tgtgATATCAGTCACATAAATGTAGGGGGTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCtt < 1:1263072/68‑1 (MQ=255)tgtgATATCAGTCACATAAATGTAGGGGGTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCtt < 1:1422686/68‑1 (MQ=255)tgtgATATCAGTCACATAAATGTAGGGGGTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCtt < 1:3158782/68‑1 (MQ=255)tgtgATATCAGTCACATAAATGTAGGGGGTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCtt < 1:3162857/68‑1 (MQ=255) atcaatcaCATAAATATAGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACGCTTGTGTaa > 1:177219/1‑68 (MQ=255) atcaatcaCATAAATATAGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACGCTTGTGTaa > 1:2189671/1‑68 (MQ=255) atcaatcaCATAAATATAGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACGCTTGTGTaa > 1:1450704/1‑68 (MQ=255) atcaatcaCATAAATATAGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACGCTTGTGTaa > 1:14034/1‑68 (MQ=255) atcaatcaCATAAATATAGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACGCTTGTGTaa > 1:672710/1‑68 (MQ=255) atcaatcaCATAAATATAGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACGCTTGTGTaa > 1:707349/1‑68 (MQ=255) tcaatcaCATAAATATAGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACGCTTGTGTaa > 1:52824/1‑67 (MQ=255) tcaatcaCATAAATATAGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACGCTTGTGTaa > 1:719218/1‑67 (MQ=255) aTAAATATAGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGcaac < 1:3414286/68‑1 (MQ=255) aTAAATATAGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGcaac < 1:2052887/68‑1 (MQ=255) aaTATAGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACg < 1:704003/47‑1 (MQ=255) aaTATAGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACg < 1:2487985/47‑1 (MQ=255) aaTATAGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACg < 1:89151/47‑1 (MQ=255) aaTATAGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACg < 1:932580/47‑1 (MQ=255) aaTATAGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACg < 1:944231/47‑1 (MQ=255) aaTATAGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACg < 1:1016466/47‑1 (MQ=255) aaTATAGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACg < 1:1750456/47‑1 (MQ=255) aaTATAGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACg < 1:119985/47‑1 (MQ=255) aaTATAGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACg < 1:1135078/47‑1 (MQ=255) atatAGGGGGT‑‑AGAGGTAATTGACGTGACGTTCATCACAAAACg < 1:2874033/44‑1 (MQ=38) ataGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGcaac < 1:1562275/62‑1 (MQ=255) taGGGGGTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCtt < 1:830985/46‑1 (MQ=38) gggggTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCTTGTGTaaaa > 1:1348522/1‑52 (MQ=38) gggggTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCTTGTGTaaaa > 1:451795/1‑52 (MQ=38) gggggTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCTTGTGTaaaa > 1:284309/1‑52 (MQ=38) gggggTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCTTGTGTaaaa > 1:2783331/1‑52 (MQ=38) gggggTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCTTGTGTaaaa > 1:582951/1‑52 (MQ=38) gggggTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCTTGTGTaaaa > 1:1177982/1‑52 (MQ=38) gggggTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCTTGTGTaaaa > 1:1108178/1‑52 (MQ=38) gggggTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCTTGTGTaaaa > 1:2154126/1‑52 (MQ=38) gggggTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCTTGTGTaaaa > 1:1922197/1‑52 (MQ=38) gggggTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCTTGTGTaaaa > 1:1961062/1‑52 (MQ=38) gggggTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCTTGTGTaaaa > 1:3297281/1‑52 (MQ=38) gggggTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCTTGTGTaaaa > 1:818856/1‑52 (MQ=38) gggggTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCTTGTGTaaaa > 1:3378825/1‑52 (MQ=38) gggggTAGAGAGGTAATTGACGTGATGCTCATCACAAAATGCTTGTGTaaaa > 1:1108059/1‑52 (MQ=38) agagagGTAATTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGAtttt > 1:4866/1‑67 (MQ=255) agagagGTAATTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTc > 1:336799/1‑68 (MQ=255) ggTAATTTACGTTACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTaaa > 1:546808/1‑69 (MQ=255) ggTAATTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTaaa < 1:2773487/69‑1 (MQ=255) ggTAATTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTaaa > 1:1585401/1‑69 (MQ=255) ggTAATTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTaa > 1:656360/1‑68 (MQ=255) gTAATTGACGTGCCGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTaaaa > 1:3186254/1‑69 (MQ=255) gTAATTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTaaaa > 1:3173288/1‑69 (MQ=255) gTAATTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTaaaa > 1:101484/1‑69 (MQ=255) gTAATTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTaaaa > 1:1526874/1‑69 (MQ=255) gTAATTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTaaaa > 1:1608997/1‑69 (MQ=255) gTAATTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTaaa < 1:552662/68‑1 (MQ=255) aaTTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAAt > 1:2960755/1‑69 (MQ=255) aaTTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAAt > 1:1418759/1‑69 (MQ=255) aaTTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAAt > 1:39578/1‑69 (MQ=255) aaTTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAAt > 1:3209167/1‑69 (MQ=255) aaTTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAAt > 1:35644/1‑69 (MQ=255) aaTTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAAt > 1:1731960/1‑69 (MQ=255) aaTTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAAt > 1:2047654/1‑69 (MQ=255) aaTTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAAt > 1:3430206/1‑69 (MQ=255) aaTTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATATTCGCTAAAAAt > 1:2948409/1‑69 (MQ=255) ttGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATg > 1:167361/1‑68 (MQ=255) tgacgtgacgTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGc > 1:31724/1‑69 (MQ=255) tgacgtgacgTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGc < 1:2952990/69‑1 (MQ=255) gacgtgacgtTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGc > 1:92958/1‑68 (MQ=255) gacgtgacgtTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGCa > 1:2494515/1‑69 (MQ=255) gacgtgacgtTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGCa > 1:1319858/1‑69 (MQ=255) acgtTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGCATTTaaa > 1:1779690/1‑69 (MQ=255) acgtTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGCATTTaaa < 1:693114/69‑1 (MQ=255) acgtTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGCATTTaaa < 1:1071301/69‑1 (MQ=255) acgtTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGCATTTaaa < 1:57489/69‑1 (MQ=255) acgtTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGCATTTaaa > 1:1383932/1‑69 (MQ=255) acgtTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCAGCGATTTTCGCTAAAAATGGCATTTaaa < 1:406137/69‑1 (MQ=255) catcaCAAAACGCTTGTGTAAAAAAGCAACAAggcggc > 1:1443718/1‑38 (MQ=255) catcaCAAAACGCTTGTGTAAAAAAGCAACAAggcggc > 1:1960076/1‑38 (MQ=255) catcaCAAAACGCTTGTGTAAAAAAGCAACAAggcggc > 1:2224698/1‑38 (MQ=255) catcaCAAAACGCTTGTGTAAAAAAGCAACAAggcg > 1:2186992/1‑36 (MQ=255) cacaAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGCATTTAAATGTGagta > 1:1822291/1‑69 (MQ=255) cacaAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGCATTTAAATGTGagta > 1:2002460/1‑69 (MQ=255) cacaAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGCATTTAAATGTGagta > 1:240408/1‑69 (MQ=255) cacaAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGCATTTAAATGTGagta > 1:706844/1‑69 (MQ=255) cacaAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGCATTTAAATGTGagta > 1:2252111/1‑69 (MQ=255) cacaAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGCATTTAAATGTGagt < 1:1965206/68‑1 (MQ=255) cacaAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGCATTTAAATGTGagt < 1:3239816/68‑1 (MQ=255) cacaAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGCATTTAAATGTGagt < 1:2840199/68‑1 (MQ=255) cacaAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGCATTTAAATGTGagt < 1:3153701/68‑1 (MQ=255) cacaAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGCATTTAAATGTGagt < 1:375962/68‑1 (MQ=255) acaAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGCATTTAAATGTGagtag > 1:2447116/1‑69 (MQ=255) | TGTGATATCAATCACATAAATATAGGGGGTAGAGAGGTAATTGACGTGACGTTCATCACAAAACGCTTGTGTAAAAAAGCAACAAGGCGGCGATTTTCGCTAAAAATGGCATTTAAATGTGAGTAG > minE/2569338‑2569463 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |