| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 221,897 | A→G | 48.3% | R77R (CGT→CGC) | fadE ← | acyl coenzyme A dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 221,897 | 0 | A | G | 48.3% | ‑5.7 / 22.8 | 29 | R77R (CGT→CGC) | fadE | acyl coenzyme A dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (9/6); new base G (7/7); total (16/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01 | |||||||||||
CCAGGTGGTGCCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGC > minE/221837‑221960 | ccaGGTGGTGCCCGCGTCGATCGCTTCTTTCTCGGTGCGCGACATCGGCGGCATCACTTTGCGGaatc > 1:1130539/1‑66 (MQ=255)ccaGGTGGTGCCCGCGTCGATCGCTTCTTTCTCGGTGCGCGACATCGGCGGCATCACTTTGCGGaatc > 1:1608239/1‑66 (MQ=255)ccaGGTGGTGCCCGCGTCGATCGCTTCTTTCTCGGTGCGCGACATCGGCGGCATCACTTTGCGGAATcc > 1:1437948/1‑69 (MQ=255)ccaGGTGGTGCCCGCGTCGATCGCTTCTTTCTCGGTGCGCGACATCGGCGGCATCACTTTGCGGAATcc > 1:784045/1‑69 (MQ=255)ccaGGTGGTGCCCGCGTCGATCGCTTCTTTCTCGGTGCGCGACATCGGCGGCATCACTTTGCGGAATcc > 1:2094578/1‑69 (MQ=255)ccaGGTGGTGCCCGCGTCGATCGCTTCTTTCTCGGTGCGCGACATCGGCGGCATCACTTTGCGGAATcc > 1:610353/1‑69 (MQ=255)ccaGGTGGTGCCCGCGTCGATCGCTTCTTTCTCGGTGCGCGACATCGGCGGCATCACTTTGCGGAATcc > 1:2399215/1‑69 (MQ=255) gtgCCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAAt < 1:3221904/69‑1 (MQ=255) tctttctCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAatcatc > 1:352390/1‑69 (MQ=255) tctttctCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAatcatc > 1:2205258/1‑69 (MQ=255) tctttctCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAatcatc > 1:284070/1‑69 (MQ=255) tctttctCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAatca > 1:3394794/1‑67 (MQ=255) tcAGTGCGCGACATCTGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACt < 1:3293500/67‑1 (MQ=255) tGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCa < 1:2365034/69‑1 (MQ=255) tGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCa < 1:69757/69‑1 (MQ=255) tGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCa < 1:2290239/69‑1 (MQ=255) tGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCa < 1:1523171/69‑1 (MQ=255) tCGGCGGCATCACTTTGCGGAATCCACGAAACACTGGCGCAGAAATCATCGATTTACGCATAg < 1:776357/63‑1 (MQ=255) tCGGCGGCATCACTTTGCGGAATCCACGAAACACTGGCGCAGAAATCATCGATTTACGCATAg < 1:331363/63‑1 (MQ=255) tCGGCGGCATCACTTTGCGGAATCCACGAAACACTGGCGCAGAAATCATCGATTTACGCATAg < 1:884244/63‑1 (MQ=255) tCGGCGGCATCACTTTGCGGAATCCACGAAACACTGGCGCAGAAATCATCGATTTACGCATAg < 1:1347032/63‑1 (MQ=255) tCGGCGGCATCACTTTGCGGAATCCACGAAACACTGGCGCAGAAATCATCGATTTACGCATAg < 1:1781360/63‑1 (MQ=255) tCGGCGGCATCACTTTGCGGAATCCACGAAACACTGGCGCAGAAATCATCGATTTACGCATAg < 1:1491537/63‑1 (MQ=255) tCGGCGGCATCACTTTGCGGAATCCACGAAACACTGGCGCAGAAATCATCGATTTACGCATAg < 1:135989/63‑1 (MQ=255) aTCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGgc > 1:2459592/1‑57 (MQ=255) aTCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAAt > 1:2912123/1‑69 (MQ=255) aTCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAAt > 1:2322196/1‑69 (MQ=255) aCCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGc > 1:3130508/1‑69 (MQ=255) aCCTTACGGAAACCGCGAAATAACGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGc > 1:3157960/1‑69 (MQ=255) | CCAGGTGGTGCCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGC > minE/221837‑221960 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |