| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,574,961 | T→C | 58.4% | R212R (CGT→CGC) | glyS → | glycine tRNA synthetase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,574,961 | 0 | T | C | 58.4% | 24.5 / 45.4 | 68 | R212R (CGT→CGC) | glyS | glycine tRNA synthetase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base T (14/14); new base C (9/30); total (24/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.61e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGGCGAGCCGGAATTCACCATCGATAACGCCGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCGATGCCGAAGAAGC > minE/2574900‑2575023 | tGGGCGAGCCGGAATTCACCATCGATAACGCCGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGaaa < 1:1767300/68‑1 (MQ=255)tGGGCGAGCCGGAATTCACCATCGATAACGCCGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGaaa < 1:2138781/68‑1 (MQ=255)tGGGCGAGCCGGAATTCACCATCGATAACGCCGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGaaa < 1:2818792/68‑1 (MQ=255) cACCATCGATAACGCCGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGATAGTCATCGCCGATTa > 1:2276609/1‑66 (MQ=255) cACCATCGATAACGCCGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTa > 1:1996328/1‑66 (MQ=255) cACCATCGATAACGCCGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTa > 1:84266/1‑66 (MQ=255) cACCATCGATAACGCCGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTa > 1:2058439/1‑66 (MQ=255) cACCATCGATAACGCCGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTa > 1:2079316/1‑66 (MQ=255) cACCATCGATAACGCCGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTa > 1:323498/1‑66 (MQ=255) cACCATCGATAACGCCGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTa > 1:2122155/1‑66 (MQ=255) cACCATCGATAACGCCGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTa > 1:3085984/1‑66 (MQ=255) cACCATCGATAACGCCGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTa > 1:1478665/1‑66 (MQ=255) gATAACGCCGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTACGAAGAAc > 1:1012317/1‑67 (MQ=255) gATAACGCCGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTACGAAGAAc > 1:543760/1‑67 (MQ=255) gATAACGCCGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTACGAAGAAc > 1:1153500/1‑67 (MQ=255) gATAACGCCGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTACGAAGAACGt < 1:139361/69‑1 (MQ=255) gATAACGCCGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTACGAAGAACGt < 1:913388/69‑1 (MQ=255) aatgCCGATCAGTATCCGGAAATTCTGCGTGAGCGGGGGAAAGTCATCGCCGATTACGAAGAACGTAAg > 1:1193824/4‑69 (MQ=255) aatgCCGATCAGTATCCGGAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTaa > 1:3070867/4‑68 (MQ=255) aatgCCGATCAGTATCCGGAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTaa > 1:1038953/4‑68 (MQ=255) aatgCCGATCAGTATCCGGAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAg > 1:1570856/4‑69 (MQ=255) aatgCCGATCAGTATCCGGAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAg > 1:2400634/4‑69 (MQ=255) aatgCCGATCAGTATCCGGAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAg > 1:2466092/4‑69 (MQ=255) aatgCCGATCAGTATCCGGAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAg > 1:2593085/4‑69 (MQ=255) aatgCCGATCAGTATCCGGAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAg > 1:2982305/4‑69 (MQ=255) aatgCCGATCAGTATCCGGAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAg > 1:1780939/4‑69 (MQ=255) aatgCCGATCAGTATCCGGAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAg > 1:3393487/4‑69 (MQ=255) cGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCTTCGCCGATTACGAAGAACGTAAGGCGaa < 1:397070/69‑1 (MQ=255) cGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGaa < 1:3211272/69‑1 (MQ=255) cGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGaa < 1:2981195/69‑1 (MQ=255) cGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGaa < 1:1537603/69‑1 (MQ=255) cGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGaa < 1:2133105/69‑1 (MQ=255) cGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGaa < 1:1186369/69‑1 (MQ=255) cGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGaa < 1:2522712/69‑1 (MQ=255) cGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGaa < 1:2438825/69‑1 (MQ=255) cGGAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGcc < 1:1293934/68‑1 (MQ=255) cGGAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGcc < 1:3058523/68‑1 (MQ=255) cGGAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGcc < 1:3227001/68‑1 (MQ=255) cGGAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGcc < 1:1460484/68‑1 (MQ=255) cGGAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGcc < 1:361088/68‑1 (MQ=255) cGGAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGcc < 1:2156694/68‑1 (MQ=255) cGGAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGcc < 1:11599/68‑1 (MQ=255) cGGAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGcc < 1:1558110/68‑1 (MQ=255) cGGAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGcc < 1:689850/68‑1 (MQ=255) cGGAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGcc < 1:1981128/68‑1 (MQ=255) cGGAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGcc < 1:1866031/68‑1 (MQ=255) cGGAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGcc < 1:1825869/68‑1 (MQ=255) ggAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGcc < 1:1102920/67‑1 (MQ=255) ggAAATTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCg < 1:1992296/68‑1 (MQ=255) aaaTTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCg < 1:404378/66‑1 (MQ=255) aaaTTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCg < 1:1640581/66‑1 (MQ=255) aaaTTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCg < 1:447178/66‑1 (MQ=255) aaaTTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCg < 1:344989/66‑1 (MQ=255) aaaTTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCg < 1:741977/66‑1 (MQ=255) aaaTTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCg < 1:1142664/66‑1 (MQ=255) aaaTTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCg < 1:3323889/66‑1 (MQ=255) aaaTTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCg < 1:326199/66‑1 (MQ=255) aaaTTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCg < 1:1367964/66‑1 (MQ=255) aaaTTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCg < 1:1402870/66‑1 (MQ=255) aaaTTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCg < 1:2383071/66‑1 (MQ=255) aaaTTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCg < 1:1464231/66‑1 (MQ=255) aaaTTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCg < 1:1600650/66‑1 (MQ=255) aaaTTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCg < 1:1937108/66‑1 (MQ=255) aaaTTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCg < 1:187158/66‑1 (MQ=255) aaaTTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCg < 1:1733154/66‑1 (MQ=255) aaTTCTGCGTGAGCGCGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCg < 1:2181883/65‑1 (MQ=255) tGAGCGTGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCGATGCCGaagaag > 1:2436848/1‑68 (MQ=255) tGAGCGTGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCGATGCCGAAGAagc > 1:9733/1‑69 (MQ=255) | TGGGCGAGCCGGAATTCACCATCGATAACGCCGATCAGTATCCGGAAATTCTGCGTGAGCGTGGGAAAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCGATGCCGAAGAAGC > minE/2574900‑2575023 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |