| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,575,573 | T→C | 44.3% | Y416Y (TAT→TAC) | glyS → | glycine tRNA synthetase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,575,573 | 0 | T | C | 44.3% | 11.9 / 55.0 | 70 | Y416Y (TAT→TAC) | glyS | glycine tRNA synthetase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base T (7/32); new base C (22/9); total (29/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.28e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
CTGATGACCAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTATGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTGAATGAGCAGTATCAGCCGCGTTTTGC > minE/2575514‑2575641 | cTGATGACCAACATGGTCTTCGAGTTCACCGACACCCATGGCGTTATGGGGATGCACTATGCGCGTCAc < 1:196115/69‑1 (MQ=255)cTGATGACCAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATTCACTATGCGCGTCAc < 1:3267420/69‑1 (MQ=255)cTGATGACCAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGTACTATGCGCGTCAc < 1:1731946/69‑1 (MQ=255)cTGATGACCAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTATGCTCGTCAc < 1:3083/69‑1 (MQ=255)cTGATGACCAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTATGCGCGTCAc < 1:103780/69‑1 (MQ=255)cTGATGACCAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTATGCGCGTCAc < 1:202066/69‑1 (MQ=255)cTGATGACCAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTATGCGCGTCAc < 1:2228262/69‑1 (MQ=255)cTGATGACCAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTATGCGCGTCAc < 1:2403431/69‑1 (MQ=255)cTGATGACCAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTATGCGCGTCAc < 1:2406089/69‑1 (MQ=255)cTGATGACCAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTATGCGCGTCAc < 1:1707525/69‑1 (MQ=255)cTGATGACCAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTATGCGCGTCAc < 1:2711041/69‑1 (MQ=255)cTGATGACCAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTATGCGCGTCAc < 1:2882493/69‑1 (MQ=255)cTGATGACCAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTATGCGCGTCAc < 1:2893535/69‑1 (MQ=255)cTGATGACCAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTATGCGCGTCAc < 1:3342994/69‑1 (MQ=255)cTGATGACCAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTATGCGCGTCAc < 1:1353478/69‑1 (MQ=255)cTGATGACCAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTATGCGCGTCAc < 1:1344410/69‑1 (MQ=255)cTGATGACCAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTATGCGCGTCAc < 1:1336429/69‑1 (MQ=255)cTGATGACCAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTATGCGCGTCAc < 1:464064/69‑1 (MQ=255) aCCAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTATGCGCGTCACGATGGc < 1:1532579/69‑1 (MQ=255) aCCAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTATGCGCGTCACGATGGc < 1:1130288/69‑1 (MQ=255) cAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTATGCGCGTCAc < 1:1089406/61‑1 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGaa > 1:2310499/1‑69 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGaa > 1:3299688/1‑69 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGaa > 1:800138/1‑69 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGaa > 1:2043488/1‑69 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGaa > 1:770356/1‑69 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGaa > 1:672057/1‑69 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGaa > 1:558578/1‑69 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGaa > 1:2536970/1‑69 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGaa > 1:3217864/1‑69 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGaa > 1:1842094/1‑69 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGaa > 1:1045940/1‑69 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGaa > 1:1088346/1‑69 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGaa > 1:1189075/1‑69 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGaa > 1:1428827/1‑69 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGaa > 1:1477424/1‑69 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGaa > 1:1496446/1‑69 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGaa > 1:1524453/1‑69 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGaa > 1:1664028/1‑69 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGa > 1:3374707/1‑68 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGa > 1:1026791/1‑68 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGa > 1:460526/1‑68 (MQ=255) aaCATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGAAGCACTACGCGCGTCACGATGGCGaa > 1:2689726/1‑69 (MQ=255) aTGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGAAGCg < 1:3410225/69‑1 (MQ=255) aTGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGAAGCg < 1:2469232/69‑1 (MQ=255) aTGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGAAGCg < 1:168941/69‑1 (MQ=255) aTGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGAAGCg < 1:3372552/69‑1 (MQ=255) aTGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGAAGCg < 1:39702/69‑1 (MQ=255) aTGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGAAGCg < 1:1633855/69‑1 (MQ=255) aTGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGAAGCg < 1:405282/69‑1 (MQ=255) aTGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGAAGCg < 1:1222322/69‑1 (MQ=255) aTGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGAAGCg < 1:431949/69‑1 (MQ=255) cACCGACACCCAGGGCGTTATGGGGATGCACTATGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGt > 1:1742239/1‑69 (MQ=255) acCCAGGGCGTTATGGGGATGCACTATGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTg < 1:2178010/69‑1 (MQ=255) acCCAGGGCGTTATGGGGATGCACTATGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTg < 1:1137730/69‑1 (MQ=255) acCCAGGGCGTTATGGGGATGCACTATGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTg < 1:792724/69‑1 (MQ=255) acCCAGGGCGTTATGGGGATGCACTATGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTg < 1:197542/69‑1 (MQ=255) acCCAGGGCGTTATGGGGATGCACTATGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTg < 1:1719163/69‑1 (MQ=255) acCCAGGGCGTTATGGGGATGCACTATGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTg < 1:346129/69‑1 (MQ=255) acCCAGGGCGTTATGGGGATGCACTATGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTg < 1:3431798/69‑1 (MQ=255) acCCAGGGCGTTATGGGGATGCACTATGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTg < 1:2215826/69‑1 (MQ=255) acCCAGGGCGTTATGGGGATGCACTATGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTg < 1:3055787/69‑1 (MQ=255) cccAGGGCGTTATGGGGATGCACTATGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTg < 1:2247097/68‑1 (MQ=255) cccAGGGCGTTATGGGGATGCACTATGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTg < 1:570621/68‑1 (MQ=255) cACTATGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTGAATGAGCAGTATCAGCCGCGt > 1:1765270/1‑69 (MQ=255) cACTATGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTGAATGAGCAGTATCAGCCGCGt > 1:2849559/1‑69 (MQ=255) cACTATGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTGAATGAGCAGTATCAGCCGCGt > 1:1817946/1‑69 (MQ=255) cACTATGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTGAATGAGCAGTATCAGCCGCGt > 1:2365051/1‑69 (MQ=255) cACTATGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTGAATGAGCAGTATCAGCCGCGt > 1:899928/1‑69 (MQ=255) cACTATGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTGAATGAGCAGTATCAGCCGCGt > 1:900046/1‑69 (MQ=255) tGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTGAATGAGCAGTATCAGCCGCGTTTTGc > 1:1415508/1‑69 (MQ=255) tGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTGAATGAGCAGTATCAGCCGCGTTTTGc > 1:413410/1‑69 (MQ=255) tGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTGAATGAGCAGTATCAGCCGCGTTTTGc > 1:1587376/1‑69 (MQ=255) tGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTGAATGAGCAGTATCAGCCGCGTTTTGc > 1:2691978/1‑69 (MQ=255) | CTGATGACCAACATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTATGCGCGTCACGATGGCGAAGCGGAAGATGTCGCGGTGGCGCTGAATGAGCAGTATCAGCCGCGTTTTGC > minE/2575514‑2575641 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |